Detailed information of TA system TA089965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 988051..989288
• Replicon: chromosome
• Accession: NZ_CP025256
• Organism: Streptococcus pneumoniae strain Xen35

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: Q97QZ1
• Locus tag: CXP32_RS05255
• Protein ID: WP_000405360.1
• Coordinates: 988527..989288 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: CXP32_RS05250
• Protein ID: WP_000579608.1
• Coordinates: 988051..988527 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP32_RS05220	983123..983977	+	855	WP_000900015.1	ADP-ribosylglycohydrolase family protein	-
CXP32_RS05225	984089..984973	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
CXP32_RS05230	985137..985499	+	363	WP_000423476.1	alpha amylase N-terminal ig-like domain-containing protein	-
CXP32_RS05235	985480..986876	+	1397	Protein_970	glycoside hydrolase family 13 protein	-
CXP32_RS05240	986962..987279	+	318	WP_000995815.1	hypothetical protein	-
CXP32_RS05245	987325..987606	+	282	WP_000266234.1	hypothetical protein	-
CXP32_RS05250	988051..988527	+	477	WP_000579608.1	helix-turn-helix domain-containing protein	Antitoxin
CXP32_RS05255	988527..989288	+	762	WP_000405360.1	toxin PezT	Toxin
CXP32_RS05260	989316..990472	+	1157	Protein_975	hypothetical protein	-
CXP32_RS05270	991304..991660	+	357	WP_000817623.1	hypothetical protein	-
CXP32_RS05275	991665..992030	+	366	WP_000401239.1	MobC family plasmid mobilization relaxosome protein	-
CXP32_RS05280	992017..993840	+	1824	WP_000237143.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_003028 Streptococcus pneumoniae TIGR4 Integrative and Conjugative Element TA010019, TA020634 968351..1022298 0	inside	Integrative and Conjugative Element	-	-	968351..1022298	53947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29129.20 Da
• Isoelectric Point: 7.5087

>T89965 WP_000405360.1 NZ_CP025256:988527-989288 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T89965 NZ_CP025256:988527-989288 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18247.70 Da
• Isoelectric Point: 4.4188

>AT89965 WP_000579608.1 NZ_CP025256:988051-988527 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT89965 NZ_CP025256:988051-988527 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTGCGTAAAACACATGACTTAACACAACTCGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
GTCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGATTTGATTCATACGAATATCTATCTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTGGCCTAA

Structures

Toxin

• PDB: 2P5T