Detailed information of TA system TA023658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 964173..965410
• Replicon: chromosome
• Accession: NC_012467
• Organism: Streptococcus pneumoniae P1031

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: SPP_RS05220
• Protein ID: WP_000405353.1
• Coordinates: 964649..965410 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: SPP_RS05215
• Protein ID: WP_000579613.1
• Coordinates: 964173..964649 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPP_RS05190	959250..960104	+	855	WP_000900020.1	ADP-ribosylglycohydrolase family protein	-
SPP_RS05195	960216..961100	+	885	WP_000710109.1	diacylglycerol kinase family lipid kinase	-
SPP_RS05200	961263..963000	+	1738	Protein_983	alpha-glycosidase	-
SPP_RS05210	963275..963730	+	456	WP_000500471.1	hypothetical protein	-
SPP_RS05215	964173..964649	+	477	WP_000579613.1	helix-turn-helix domain-containing protein	Antitoxin
SPP_RS05220	964649..965410	+	762	WP_000405353.1	zeta toxin family protein	Toxin
SPP_RS05225	965438..966595	+	1158	WP_001213953.1	hypothetical protein	-
SPP_RS05230	967414..967773	+	360	WP_000003746.1	hypothetical protein	-
SPP_RS05235	967849..968147	+	299	Protein_989	MobC family plasmid mobilization relaxosome protein	-
SPP_RS13120	968134..969998	+	1865	Protein_990	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_018630 Streptococcus pneumoniae gamPNI0373 Genomic island TA006080 1035089..1069396 0.005	inside	Genomic island	-	-	948920..980083	31163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29043.02 Da
• Isoelectric Point: 7.2344

>T23658 WP_000405353.1 NC_012467:964649-965410 [Streptococcus pneumoniae P1031]
VEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTELSHLGYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLHELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T23658 NC_012467:964649-965410 [Streptococcus pneumoniae P1031]
GTGGAAATCCAAGATTATACTGACAGTGAATTCAAACATGCTCTAGCACGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGTGTAGAATACACCAAAGATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAACAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACTCTTATTCGATACGAAGAA
CTGTACGCTATCAATCCAAATCAAGCACGCGCAACACCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATTTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCATGAGTTATTGTTTGGAGAGTGGAGTCAGGTAGAAAAGGAGATGTTG
AGGGTAGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18249.67 Da
• Isoelectric Point: 4.3462

>AT23658 WP_000579613.1 NC_012467:964173-964649 [Streptococcus pneumoniae P1031]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT23658 NC_012467:964173-964649 [Streptococcus pneumoniae P1031]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCTGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCAGACAAATATCTATTTAGTAGAAAATTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAG