Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pezAT/zeta-couple_hipB
Location 1020962..1022199 Replicon chromosome
Accession NZ_CP035238
Organism Streptococcus pneumoniae strain TVO_D39

Toxin (Protein)


Gene name pezT Uniprot ID R4H7C0
Locus tag EQH42_RS05040 Protein ID WP_000405357.1
Coordinates 1020962..1021723 (-) Length 254 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID B6ZCC4
Locus tag EQH42_RS05045 Protein ID WP_000579612.1
Coordinates 1021723..1022199 (-) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH42_RS05015 1016340..1018225 - 1886 Protein_1003 SAG1250 family conjugative relaxase -
EQH42_RS05020 (EQH42_05315) 1018248..1018577 - 330 WP_000263037.1 plasmid mobilization relaxosome protein MobC -
EQH42_RS05025 (EQH42_05320) 1018587..1018946 - 360 WP_000529543.1 SAG1252 family conjugative relaxosome accessory protein -
EQH42_RS05030 1019778..1020155 - 378 WP_000530001.1 hypothetical protein -
EQH42_RS05035 (EQH42_05330) 1020158..1020934 - 777 WP_001821017.1 hypothetical protein -
EQH42_RS05040 (EQH42_05335) 1020962..1021723 - 762 WP_000405357.1 type II toxin-antitoxin system toxin PezT Toxin
EQH42_RS05045 (EQH42_05340) 1021723..1022199 - 477 WP_000579612.1 type II toxin-antitoxin system antitoxin PezA Antitoxin
EQH42_RS05050 1022644..1023099 - 456 Protein_1010 hypothetical protein -
EQH42_RS05055 (EQH42_05355) 1023374..1025113 - 1740 WP_000423451.1 glycoside hydrolase family 13 protein -
EQH42_RS05060 (EQH42_05360) 1025276..1026160 - 885 WP_000710119.1 diacylglycerol kinase family lipid kinase -
EQH42_RS05065 (EQH42_05365) 1026272..1027126 - 855 WP_000900020.1 ADP-ribosylglycohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1002490..1037454 34964


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(25-215)

Antitoxin

Cro/C1-type helix-turn-helix domain

(3-60)


Sequences

Toxin        


Download         Length: 254 a.a.        Molecular weight: 29044.10 Da        Isoelectric Point: 7.1307

>T117437 WP_000405357.1 NZ_CP035238:c1021723-1020962 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Download         Length: 762 bp

>T117437 NZ_CP035238:c1021723-1020962 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACAATTGATGTTCCAAAGAAAACGGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
CACACGAAAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
CAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 18245.68 Da        Isoelectric Point: 4.4188

>AT117437 WP_000579612.1 NZ_CP035238:c1022199-1021723 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Download         Length: 477 bp

>AT117437 NZ_CP035238:c1022199-1021723 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACAGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4H7C0

Antitoxin

Source ID Structure
AlphaFold DB B6ZCC4

References