Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00272  |
| T6SS type | Type i1 |
| Strain | Proteus mirabilis HI4320 |
| Replicon (RefSeq) | chromosome (NC_010554) |
| Location | 807789..834462 |
| Function | anti-bacterial |
| Comment | In contrast to the current model of T6SS being an offensive or defensive weapon our findings support a preemptive mechanism by which an entire population indiscriminately uses the T6SS for contact-dependent delivery of effectors during its cooperative mode of growth. |
| References | [1] Wenren LM et al (2013) Two Independent Pathways for Self-Recognition in Proteus mirabilis Are Linked by Type VI-Dependent Export. MBio. 4(4). [PudMed:23882014] [2] Alteri CJ et al (2013) Multicellular Bacteria Deploy the Type VI Secretion System to Preemptively Strike Neighboring Cells. PLoS Pathog. 9(9):e1003608. [PudMed:24039579] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | FHA | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssJ | TssK | TssL | TssM |
| Number | 1 | 2 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| PMI_RS03585 (aphA) | 803332..804045 | + | 714 | WP_004247637.1 | acid phosphatase AphA | ||
| PMI_RS03590 (ldtD) | 804395..806110 | + | 1716 | WP_012367716.1 | L,D-transpeptidase | ||
| PMI_RS03595 | 806448..806996 | + | 549 | WP_004244605.1 | YcbK family protein | ||
| PMI_RS03600 | 807046..807696 | + | 651 | WP_012367717.1 | MBL fold metallo-hydrolase | ||
| T6CP008690 | TssD | PMI_RS03605 | 807789..808262 | - | 474 | WP_004244607.1 | Hcp family type VI secretion system effector |
| T6CP002716 | TssF | PMI_RS03610 | 808353..810089 | - | 1737 | WP_012367718.1 | type VI secretion system baseplate subunit TssF |
| T6CP008691 | TssA | PMI_RS03615 | 810082..811437 | - | 1356 | WP_012367719.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
| T6CP002717 | TssM | PMI_RS03620 (tssM) | 811475..815023 | - | 3549 | WP_004244610.1 | type VI secretion system membrane subunit TssM |
| T6CP008692 | TssA | PMI_RS03625 (tssA) | 815026..816489 | - | 1464 | WP_012367720.1 | type VI secretion system protein TssA |
| PMI_RS03630 (tagO) | 816495..817145 | - | 651 | WP_004244612.1 | type VI secretion system-associated protein TagO | ||
| PMI_RS03635 | 817147..817935 | - | 789 | WP_004244614.1 | hypothetical protein | ||
| T6CP002718 | TssH | PMI_RS03640 (tssH) | 817939..820650 | - | 2712 | WP_012367721.1 | type VI secretion system ATPase TssH |
| T6CP002719 | TssL | PMI_RS03645 (icmH) | 820659..821414 | - | 756 | WP_004244617.1 | type IVB secretion system protein IcmH/DotU |
| T6CP002720 | TssK | PMI_RS03650 (tssK) | 821407..822765 | - | 1359 | WP_004247647.1 | type VI secretion system baseplate subunit TssK |
| T6CP002721 | TssJ | PMI_RS03655 (tssJ) | 822767..823318 | - | 552 | WP_004244619.1 | type VI secretion system lipoprotein TssJ |
| T6CP011670 | FHA | PMI_RS03660 (tagH) | 823320..824588 | - | 1269 | WP_012367722.1 | type VI secretion system-associated FHA domain protein TagH |
| T6CP002722 | TssG | PMI_RS03665 (tssG) | 824593..825630 | - | 1038 | WP_004244621.1 | type VI secretion system baseplate subunit TssG |
| T6CP002723 | TssF | PMI_RS03670 (tssF) | 825594..827369 | - | 1776 | WP_012367723.1 | type VI secretion system baseplate subunit TssF |
| T6CP008693 | TssE | PMI_RS03675 (tssE) | 827377..827808 | - | 432 | WP_004244624.1 | type VI secretion system baseplate subunit TssE |
| T6CP002724 | TssC | PMI_RS03680 (tssC) | 827814..829292 | - | 1479 | WP_004247652.1 | type VI secretion system contractile sheath large subunit |
| T6CP002725 | TssB | PMI_RS03685 (tssB) | 829313..829813 | - | 501 | WP_004247653.1 | type VI secretion system contractile sheath small subunit |
| T6CP008694 | TssD | PMI_RS03690 | 831710..832228 | + | 519 | WP_012367724.1 | Hcp family type VI secretion system effector |
| PMI_RS03695 (tssI) | 832312..834360 | + | 2049 | pseudogene | type VI secretion system tip protein VgrG | ||
| PMI_RS03700 | 834465..834995 | + | 531 | WP_004244630.1 | hypothetical protein | ||
| PMI_RS03705 | 835010..836065 | + | 1056 | WP_004244631.1 | DUF2169 domain-containing protein | ||
| PMI_RS03710 | 836068..837093 | + | 1026 | WP_012367726.1 | 3-oxoacyl-ACP synthase | ||
| PMI_RS03715 | 837094..838668 | + | 1575 | WP_012367727.1 | DUF4150 domain-containing protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0201 |
PMI0271 (MrpJ) | protein | Global regulator | Proteus mirabilis HI4320 | chromosome (NC_010554) | 318458..318781 (+) | 490365253 | PMI0749 (tssA); PMI2990 (idsA/hcp) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01807 |
PMI0755 (pefD) | Tde | IMU00325 ; IMU00326 |
Proteus mirabilis HI4320 | chromosome (NC_010554) | 837094..838668 (+) | 501336092 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00325 |
PMI0756 (PefE) | EFF01807 |
Proteus mirabilis HI4320 | chromosome (NC_010554) | 838672..839634 (+) | 501336093 |
| IMU00326 |
PMI0762 (PefE2) | EFF01807 |
Proteus mirabilis HI4320 | chromosome (NC_010554) | 845795..846757 (+) | 194682143 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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