Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00272 experimental
T6SS type Type i1
Strain Proteus mirabilis HI4320
Replicon (RefSeq) chromosome (NC_010554)
Location 807789..834462
Function anti-bacterial
Comment In contrast to the current model of T6SS being an offensive or defensive weapon our findings support a preemptive mechanism by which an entire population indiscriminately uses the T6SS for contact-dependent delivery of effectors during its cooperative mode of growth.
References
[1] Wenren LM et al (2013) Two Independent Pathways for Self-Recognition in Proteus mirabilis Are Linked by Type VI-Dependent Export. MBio. 4(4). [PudMed:23882014]
[2] Alteri CJ et al (2013) Multicellular Bacteria Deploy the Type VI Secretion System to Preemptively Strike Neighboring Cells. PLoS Pathog. 9(9):e1003608. [PudMed:24039579]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHATssATssBTssCTssDTssETssFTssGTssHTssJTssKTssLTssM
Number1211212111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PMI_RS03585 (aphA) 803332..804045 + 714 WP_004247637.1 acid phosphatase AphA
PMI_RS03590 (ldtD) 804395..806110 + 1716 WP_012367716.1 L,D-transpeptidase
PMI_RS03595 806448..806996 + 549 WP_004244605.1 YcbK family protein
PMI_RS03600 807046..807696 + 651 WP_012367717.1 MBL fold metallo-hydrolase
T6CP008690 TssD PMI_RS03605 807789..808262 - 474 WP_004244607.1 Hcp family type VI secretion system effector
T6CP002716 TssF PMI_RS03610 808353..810089 - 1737 WP_012367718.1 type VI secretion system baseplate subunit TssF
T6CP008691 TssA PMI_RS03615 810082..811437 - 1356 WP_012367719.1 type VI secretion system ImpA family N-terminal domain-containing protein
T6CP002717 TssM PMI_RS03620 (tssM) 811475..815023 - 3549 WP_004244610.1 type VI secretion system membrane subunit TssM
T6CP008692 TssA PMI_RS03625 (tssA) 815026..816489 - 1464 WP_012367720.1 type VI secretion system protein TssA
PMI_RS03630 (tagO) 816495..817145 - 651 WP_004244612.1 type VI secretion system-associated protein TagO
PMI_RS03635 817147..817935 - 789 WP_004244614.1 hypothetical protein
T6CP002718 TssH PMI_RS03640 (tssH) 817939..820650 - 2712 WP_012367721.1 type VI secretion system ATPase TssH
T6CP002719 TssL PMI_RS03645 (icmH) 820659..821414 - 756 WP_004244617.1 type IVB secretion system protein IcmH/DotU
T6CP002720 TssK PMI_RS03650 (tssK) 821407..822765 - 1359 WP_004247647.1 type VI secretion system baseplate subunit TssK
T6CP002721 TssJ PMI_RS03655 (tssJ) 822767..823318 - 552 WP_004244619.1 type VI secretion system lipoprotein TssJ
T6CP011670 FHA PMI_RS03660 (tagH) 823320..824588 - 1269 WP_012367722.1 type VI secretion system-associated FHA domain protein TagH
T6CP002722 TssG PMI_RS03665 (tssG) 824593..825630 - 1038 WP_004244621.1 type VI secretion system baseplate subunit TssG
T6CP002723 TssF PMI_RS03670 (tssF) 825594..827369 - 1776 WP_012367723.1 type VI secretion system baseplate subunit TssF
T6CP008693 TssE PMI_RS03675 (tssE) 827377..827808 - 432 WP_004244624.1 type VI secretion system baseplate subunit TssE
T6CP002724 TssC PMI_RS03680 (tssC) 827814..829292 - 1479 WP_004247652.1 type VI secretion system contractile sheath large subunit
T6CP002725 TssB PMI_RS03685 (tssB) 829313..829813 - 501 WP_004247653.1 type VI secretion system contractile sheath small subunit
T6CP008694 TssD PMI_RS03690 831710..832228 + 519 WP_012367724.1 Hcp family type VI secretion system effector
PMI_RS03695 (tssI) 832312..834360 + 2049 pseudogene type VI secretion system tip protein VgrG
PMI_RS03700 834465..834995 + 531 WP_004244630.1 hypothetical protein
PMI_RS03705 835010..836065 + 1056 WP_004244631.1 DUF2169 domain-containing protein
PMI_RS03710 836068..837093 + 1026 WP_012367726.1 3-oxoacyl-ACP synthase
PMI_RS03715 837094..838668 + 1575 WP_012367727.1 DUF4150 domain-containing protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0201 experimental PMI0271 (MrpJ) protein Global regulator Proteus mirabilis HI4320 chromosome (NC_010554) 318458..318781 (+) 490365253 PMI0749 (tssA); PMI2990 (idsA/hcp)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01807 experimental PMI0755 (pefD) Tde IMU00325 experimental; IMU00326 experimental Proteus mirabilis HI4320 chromosome (NC_010554) 837094..838668 (+) 501336092

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00325 experimental PMI0756 (PefE) EFF01807 experimental Proteus mirabilis HI4320 chromosome (NC_010554) 838672..839634 (+) 501336093
IMU00326 experimental PMI0762 (PefE2) EFF01807 experimental Proteus mirabilis HI4320 chromosome (NC_010554) 845795..846757 (+) 194682143

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector