SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002716
Component protein type	TssF
T6SS ID (Type)	T6SS00272 (Type i1)
Strain	Proteus mirabilis HI4320
Replicon	chromosome
Sequence	Protein sequence (579 a.a.); Nucleotide sequence (1737 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PMI_RS03610
Coordinate (Strand)	808353..810089 (-)
NCBI ID	WP_012367718.1
RefSeq	NC_010554
Uniprot ID	B4ET46_PROMH
KEGG ID	pmr:PMI0734
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.3e-42	6..561

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-578	MSEIKYIKEKQYLQKLFSEYADKAPHLASVLDPQDPQTSYLLEGFAFLSARLQDKIDDAFPEITLPLLQRLNSQAIKGLPSTTIIQIDQSEILPYPMEINEKHLVIGDNGAQFSFCHNFTIMPYSILDRKITQHPNRSCISLEILYRGDVELTQTNALNVFLGENKTTSDILLLAFSQYFEKIEVVHNGERYEGDPLNYAFEPKIGNDYKIFPQKNNSFSAPQRLLEGLYLPHVHHFIELDIPQIVTQLDWKQQQRFVVNIYFSQQLPLTQEECNQSFFLNCAPAIDSEAKHTLLIDFKKNKSSYLLPIPTHHYLADLDKIELSLEPHELARGIYCHFYPTTELTAASRLMPQFHKAIFYSLTMEKNITGHTLYYLNFFDNKGDPMVTPPSLHFACVYIGFEQYKRNELGLLNKHSEKMPDAVKTGNITLLSSCYPPIVNNHHFWKLLSHYSANGSMLMSLDTIKHMISDYILYRDTDRQITRKCERLLNGLVELKTHLYDYILKGKPYRCLSLSLFIDETQYENRGEAFVFTTHLYHFFPFCLSENMLLEMSVTLNDQKNTTWYLSPSPLRGYKSMI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9981	-	-

Protein sequence: 579 a.a.

						>T6CP002716 NC_010554:c810089-808353 [Proteus mirabilis HI4320] [TssF]
MSEIKYIKEKQYLQKLFSEYADKAPHLASVLDPQDPQTSYLLEGFAFLSARLQDKIDDAFPEITLPLLQRLNSQAIKGLP
STTIIQIDQSEILPYPMEINEKHLVIGDNGAQFSFCHNFTIMPYSILDRKITQHPNRSCISLEILYRGDVELTQTNALNV
FLGENKTTSDILLLAFSQYFEKIEVVHNGERYEGDPLNYAFEPKIGNDYKIFPQKNNSFSAPQRLLEGLYLPHVHHFIEL
DIPQIVTQLDWKQQQRFVVNIYFSQQLPLTQEECNQSFFLNCAPAIDSEAKHTLLIDFKKNKSSYLLPIPTHHYLADLDK
IELSLEPHELARGIYCHFYPTTELTAASRLMPQFHKAIFYSLTMEKNITGHTLYYLNFFDNKGDPMVTPPSLHFACVYIG
FEQYKRNELGLLNKHSEKMPDAVKTGNITLLSSCYPPIVNNHHFWKLLSHYSANGSMLMSLDTIKHMISDYILYRDTDRQ
ITRKCERLLNGLVELKTHLYDYILKGKPYRCLSLSLFIDETQYENRGEAFVFTTHLYHFFPFCLSENMLLEMSVTLNDQK
NTTWYLSPSPLRGYKSMI*					

Nucleotide sequence: 1737 bp

						>T6CP002716 NC_010554:c810089-808353 [Proteus mirabilis HI4320] [TssF]
ATGAGTGAAATTAAATATATTAAAGAAAAGCAGTACTTACAAAAACTGTTCAGTGAGTATGCAGATAAAGCGCCACATTT
AGCGAGCGTACTTGATCCTCAAGATCCACAAACAAGTTATCTCTTAGAAGGCTTTGCTTTTTTATCTGCTCGTTTACAAG
ATAAAATTGATGATGCTTTTCCTGAGATCACATTACCCCTACTGCAACGACTAAATTCCCAAGCCATTAAAGGCCTACCA
TCAACAACAATTATCCAAATTGACCAAAGTGAAATTCTCCCCTATCCCATGGAGATTAATGAAAAACATTTGGTGATTGG
CGACAACGGGGCACAATTTAGTTTCTGCCATAATTTCACCATCATGCCTTATAGCATTTTGGATAGAAAAATTACTCAGC
ATCCAAACCGCTCTTGTATCTCTCTTGAAATATTATATCGTGGTGATGTGGAATTAACTCAAACAAATGCACTTAATGTT
TTTTTAGGGGAAAATAAAACCACCTCAGACATTTTATTACTCGCATTTAGTCAATATTTTGAAAAAATTGAGGTGGTACA
TAACGGTGAACGTTATGAAGGCGATCCTCTTAATTATGCTTTTGAACCCAAAATTGGCAATGACTATAAAATCTTCCCAC
AGAAAAATAACTCATTTTCTGCACCACAACGTTTATTAGAAGGTCTTTATTTACCCCATGTTCACCATTTTATTGAGTTA
GATATACCACAAATAGTGACTCAATTAGATTGGAAACAACAGCAGCGGTTTGTTGTGAATATCTATTTCAGTCAGCAACT
TCCTTTAACTCAAGAAGAGTGTAATCAGAGCTTTTTTTTAAACTGTGCTCCAGCGATTGATAGTGAAGCAAAACATACAC
TTTTAATTGATTTTAAAAAGAATAAATCAAGTTATTTACTCCCTATTCCAACCCATCACTATTTGGCCGATCTTGATAAA
ATTGAGTTATCACTAGAGCCTCATGAGCTAGCGCGTGGCATATACTGTCATTTTTATCCTACGACAGAATTAACCGCCGC
CTCTCGTTTAATGCCCCAATTTCATAAAGCCATTTTCTATTCATTAACAATGGAGAAAAATATTACTGGGCATACACTTT
ATTATTTGAATTTCTTTGATAATAAAGGTGATCCTATGGTCACACCGCCTAGTTTACATTTTGCATGTGTTTATATTGGC
TTTGAACAATATAAAAGAAATGAGTTAGGTCTATTAAATAAACACAGCGAAAAAATGCCTGATGCAGTAAAAACAGGAAA
TATCACATTATTATCATCTTGTTATCCACCTATTGTAAATAATCACCATTTTTGGAAATTGTTATCTCATTACAGTGCTA
ATGGTTCAATGTTAATGTCATTAGATACTATTAAACATATGATTTCTGATTATATTCTTTACCGAGATACTGATAGGCAG
ATCACTCGAAAATGTGAGCGATTATTAAATGGACTCGTTGAACTAAAAACACATTTATATGACTATATTTTAAAAGGTAA
ACCCTATCGTTGTTTATCTTTATCGCTATTCATTGACGAAACACAATATGAAAATAGAGGTGAAGCTTTTGTCTTTACTA
CACATCTTTACCATTTTTTCCCGTTCTGTTTATCTGAAAATATGTTATTGGAAATGTCAGTTACACTCAACGATCAGAAA
AACACCACTTGGTATTTATCTCCCTCACCACTAAGGGGCTATAAATCAATGATTTAA					