Detailed information of component protein


Summary

Component ID T6CP008691
Component protein type TssA
T6SS ID (Type) T6SS00272 experimental (Type i1)
Strain Proteus mirabilis HI4320
Replicon chromosome
Sequence Protein sequence (452 a.a.); Nucleotide sequence (1356 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PMI_RS03615
Coordinate (Strand) 810082..811437 (-)
NCBI ID WP_012367719.1
RefSeq NC_010554
Uniprot ID B4ET47_PROMH
KEGG ID pmr:PMI0735
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 2.5e-35 287..430
ImpA_N PF06812.14 2.5e-19 15..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-234 MLSAMKSESLVIKIGGSPLDTPEFIALKTEFNKLSHPARPEISWTLIESLCLSLFKNHGIDLQSGAYYTIARLQQHGLSGFTEGCELLANVIVTYWDSLWPEKPNQRTEVLNWFNTKASALLRQQSYQAKDLRLIYRSERALQLIIDKLAKTTWEKLPKIENLLWFFQNLAKQIEKQEKQTQQDSAEPVSVPPLVCIQQPSKTTDKAAPTFVIETPQPASSFTVVAKKMSATRG
Transmembrane helix 235-257 FIWGCVLSCILFLAIGFGVHLYL
Outside 258-451 KQEMNAITSIPEGAAAKWLFKPQLNSYEYHLALLEKRSPLSNLRLIDTMQEKAKNAWPTDAEQIYFTRNWQNLYQTRLENMPINDSWSETATLLQQLSNKIVQQERNRGSFTLSYLKTAIYDIQKQHNKVEPIEEKLRQLAVQIETGQPISPATLNNIDNKINGLLARYYDLQKQAEQQGLKPGSYSSFGLDHE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9916 - -


  Protein sequence: 452 a.a.    

>T6CP008691 NC_010554:c811437-810082 [Proteus mirabilis HI4320] [TssA]
MLSAMKSESLVIKIGGSPLDTPEFIALKTEFNKLSHPARPEISWTLIESLCLSLFKNHGIDLQSGAYYTIARLQQHGLSG
FTEGCELLANVIVTYWDSLWPEKPNQRTEVLNWFNTKASALLRQQSYQAKDLRLIYRSERALQLIIDKLAKTTWEKLPKI
ENLLWFFQNLAKQIEKQEKQTQQDSAEPVSVPPLVCIQQPSKTTDKAAPTFVIETPQPASSFTVVAKKMSATRGFIWGCV
LSCILFLAIGFGVHLYLKQEMNAITSIPEGAAAKWLFKPQLNSYEYHLALLEKRSPLSNLRLIDTMQEKAKNAWPTDAEQ
IYFTRNWQNLYQTRLENMPINDSWSETATLLQQLSNKIVQQERNRGSFTLSYLKTAIYDIQKQHNKVEPIEEKLRQLAVQ
IETGQPISPATLNNIDNKINGLLARYYDLQKQAEQQGLKPGSYSSFGLDHE*

  Nucleotide sequence: 1356 bp    

>T6CP008691 NC_010554:c811437-810082 [Proteus mirabilis HI4320] [TssA]
ATGTTGAGTGCGATGAAATCAGAAAGTTTAGTGATTAAAATTGGGGGATCTCCACTTGATACCCCTGAATTTATTGCCCT
GAAAACCGAATTTAATAAATTGAGCCACCCTGCTCGACCAGAAATAAGTTGGACACTTATTGAGTCACTTTGCTTATCCC
TATTTAAAAATCATGGTATCGACCTACAAAGCGGTGCTTACTATACCATTGCAAGATTACAACAGCATGGTTTAAGCGGT
TTTACTGAAGGATGTGAGTTATTAGCAAATGTTATTGTGACTTATTGGGATAGCCTGTGGCCGGAAAAGCCAAATCAACG
TACAGAGGTATTAAACTGGTTTAATACCAAAGCCAGCGCTTTATTAAGACAACAATCCTATCAAGCAAAAGATCTACGAT
TAATCTATCGTTCAGAGCGCGCGTTACAGTTAATAATCGATAAACTGGCTAAAACAACTTGGGAAAAACTGCCTAAAATT
GAAAACTTACTGTGGTTTTTCCAAAACTTAGCCAAACAAATTGAAAAGCAAGAAAAACAGACGCAACAAGATAGTGCCGA
ACCCGTTAGCGTACCGCCATTAGTCTGTATTCAACAACCGTCGAAAACAACGGACAAAGCAGCCCCCACTTTTGTGATTG
AAACACCTCAACCCGCCTCTTCTTTCACTGTAGTAGCAAAAAAAATGAGTGCGACCCGAGGTTTTATATGGGGCTGTGTG
CTCAGTTGCATACTGTTTTTAGCTATCGGATTTGGTGTACATCTCTATTTAAAACAAGAGATGAATGCGATTACTTCAAT
ACCTGAAGGTGCGGCTGCCAAATGGTTGTTTAAACCACAGTTAAATAGCTACGAGTATCATTTAGCATTATTAGAAAAAC
GTTCTCCTCTTTCTAATTTACGGCTAATTGATACGATGCAAGAAAAAGCCAAAAACGCTTGGCCTACAGATGCAGAGCAA
ATTTATTTTACACGTAATTGGCAAAATCTTTATCAAACGCGCTTAGAGAATATGCCAATTAATGATAGCTGGTCTGAAAC
CGCGACATTATTACAACAACTTTCTAATAAAATAGTTCAGCAAGAACGAAATAGAGGCAGTTTTACACTCTCTTATCTAA
AAACGGCAATTTATGATATTCAAAAACAGCACAATAAAGTTGAGCCAATCGAAGAAAAACTGCGACAACTCGCTGTACAA
ATTGAAACAGGGCAACCTATTTCTCCTGCAACTCTTAATAATATTGATAATAAAATTAATGGGTTATTAGCTCGTTATTA
TGATTTACAAAAACAAGCTGAACAACAAGGTTTAAAACCCGGCTCATATTCATCTTTTGGATTAGATCATGAGTGA