SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00326
Cognate effector	EFF01807
Related T6SS (Type)	T6SS00272 (Type i1)
Strain	Proteus mirabilis HI4320
Replicon	chromosome
Sequence	Protein sequence (321 a.a.); Nucleotide sequence (963 bp)
Description	PefE2 protects the toxicity of PefD from P. mirabilis strain BA6163
Reference	[1] Alteri CJ, Himpsl SD, Zhu K, et al. Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity. PLoS Pathog. 2017 Nov 20;13(11):e1006729. doi: 10.1371/journal.ppat.1006729. eCollection 2017 Nov. PMID: 29155899

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PMI0762 (PefE2)
Coordinates (Strand)	845795..846757 (+)
NCBI ID	194682143
RefSeq	NC_010554
Uniprot ID	B4EVB5_PROMH
KEGG ID	pmr:PMI0762
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-320	MEYITNKHWAKYIKGYIFTGCAIRDKNIVYFCLRKDIPYEKARNLWNSEIPSQLLCIYLDTPDDPIDYIRIEGFDNPKVGICLKPKEQGMLASRGDGGFVKCMGSGSKFPLEKVMDGDYANPKNIKCVGGYAYYVGTSREIYKRVDIGKWQRIQHGIPGGETNTDMGFTDLDGFSEQDMYAVGGAGDVWHYNGEIWIPCGFPSNEQLSAVLCAPDGYVYIGGEGGSLWRGREYQWEKLYHGSSAILLNQLRWFEDKVWACDDYRLQCWNGKEMVRPKDGDRNVIASGHMDVRDGILVVADDYNVDLYDGTAWHKIVRPYK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8424	-	-

Protein sequence: 321 a.a. .

                                    >IMU00326 NC_010554:845795-846757 [Proteus mirabilis HI4320]
MEYITNKHWAKYIKGYIFTGCAIRDKNIVYFCLRKDIPYEKARNLWNSEIPSQLLCIYLDTPDDPIDYIRIEGFDNPKVG
ICLKPKEQGMLASRGDGGFVKCMGSGSKFPLEKVMDGDYANPKNIKCVGGYAYYVGTSREIYKRVDIGKWQRIQHGIPGG
ETNTDMGFTDLDGFSEQDMYAVGGAGDVWHYNGEIWIPCGFPSNEQLSAVLCAPDGYVYIGGEGGSLWRGREYQWEKLYH
GSSAILLNQLRWFEDKVWACDDYRLQCWNGKEMVRPKDGDRNVIASGHMDVRDGILVVADDYNVDLYDGTAWHKIVRPYK
*

                                

Nucleotide sequence: 963 bp .

                                    >IMU00326 NC_010554:845795-846757 [Proteus mirabilis HI4320]
ATGGAGTATATAACTAATAAACATTGGGCCAAATATATCAAGGGATATATTTTTACTGGTTGTGCAATCCGAGATAAAAA
TATTGTGTACTTTTGTTTGCGCAAAGATATTCCATACGAAAAGGCTCGAAATTTATGGAACAGCGAAATCCCCTCACAGT
TATTATGTATTTATTTAGACACACCAGATGACCCGATTGATTATATTAGAATTGAAGGGTTCGATAATCCTAAAGTAGGT
ATTTGTTTAAAACCGAAAGAACAAGGGATGCTTGCATCTAGAGGCGATGGTGGGTTTGTCAAGTGTATGGGCAGTGGATC
TAAATTTCCGCTAGAAAAAGTTATGGATGGTGACTATGCAAATCCTAAAAATATTAAATGTGTTGGTGGATATGCTTATT
ATGTTGGTACTTCGCGCGAAATTTATAAACGAGTGGATATTGGAAAATGGCAGAGAATTCAACATGGGATACCAGGAGGA
GAAACCAATACAGATATGGGATTCACTGATCTCGATGGTTTCTCTGAGCAGGATATGTATGCCGTTGGCGGTGCAGGTGA
TGTTTGGCATTATAATGGCGAAATCTGGATCCCTTGTGGTTTTCCCTCTAATGAGCAATTAAGTGCAGTATTGTGTGCCC
CTGATGGTTATGTGTATATCGGCGGTGAAGGGGGCAGTTTATGGCGAGGGCGTGAATACCAATGGGAAAAACTTTATCAC
GGCAGTTCGGCTATTTTACTGAATCAGTTACGCTGGTTTGAAGATAAAGTGTGGGCATGTGATGACTATCGCTTACAGTG
TTGGAATGGTAAAGAAATGGTCCGTCCCAAGGATGGTGATAGAAATGTTATCGCCTCAGGTCATATGGATGTCCGCGATG
GGATTTTAGTTGTAGCTGATGATTATAATGTTGATCTGTATGATGGAACCGCTTGGCATAAGATAGTGAGACCTTATAAA
TAA

                                