SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00325
Cognate effector	EFF01807
Related T6SS (Type)	T6SS00272 (Type i1)
Strain	Proteus mirabilis HI4320
Replicon	chromosome
Sequence	Protein sequence (321 a.a.); Nucleotide sequence (963 bp)
Description	PefE protects from the toxicity of PefD
Reference	[1] Alteri CJ, Himpsl SD, Zhu K, et al. Subtle variation within conserved effector operon gene products contributes to T6SS-mediated killing and immunity. PLoS Pathog. 2017 Nov 20;13(11):e1006729. doi: 10.1371/journal.ppat.1006729. eCollection 2017 Nov. PMID: 29155899

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PMI0756 (PefE)
Coordinates (Strand)	838672..839634 (+)
NCBI ID	501336093
RefSeq	NC_010554
Uniprot ID	B4EVA9_PROMH
KEGG ID	pmr:PMI0756
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-320	MTYIKEKTWKEVLKGWIITGCAIRNKNIVYFCLRKDIPYEKARNLWNSEIPSMLLAIYLDTPVTPTGYASITGFDNPKVGVCLKPKEQGMLASRGDDGVVVCVGSGANLPPEKVMDGDYANPKNIKCVGGYAYYVGSARKIYKRVDIGKWQRIQHGIPGGETNTDMGFTDLDGFSEQDMYAVGGEGDVWHYNGEIWIPCGFPSNEQLSAVLCAPDGYVYIGGEGGSLWCGREYQWEKLYHGSSTILLNQLRWFEDKVWACDDYRLQCWNGKEMVRPKDGDKNVIASGHMDVRDGILVVADDYNVDLYDGTAWHKIVRPYK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8056	-	-

Protein sequence: 321 a.a. .

                                    >IMU00325 NC_010554:838672-839634 [Proteus mirabilis HI4320]
MTYIKEKTWKEVLKGWIITGCAIRNKNIVYFCLRKDIPYEKARNLWNSEIPSMLLAIYLDTPVTPTGYASITGFDNPKVG
VCLKPKEQGMLASRGDDGVVVCVGSGANLPPEKVMDGDYANPKNIKCVGGYAYYVGSARKIYKRVDIGKWQRIQHGIPGG
ETNTDMGFTDLDGFSEQDMYAVGGEGDVWHYNGEIWIPCGFPSNEQLSAVLCAPDGYVYIGGEGGSLWCGREYQWEKLYH
GSSTILLNQLRWFEDKVWACDDYRLQCWNGKEMVRPKDGDKNVIASGHMDVRDGILVVADDYNVDLYDGTAWHKIVRPYK
*

                                

Nucleotide sequence: 963 bp .

                                    >IMU00325 NC_010554:838672-839634 [Proteus mirabilis HI4320]
ATGACATATATAAAAGAAAAAACTTGGAAAGAGGTTCTGAAAGGTTGGATAATTACTGGTTGTGCAATCCGAAATAAAAA
TATTGTGTACTTTTGTTTGCGCAAAGATATTCCATACGAAAAGGCTCGAAACTTATGGAACAGTGAAATACCTAGTATGT
TATTAGCTATCTATTTAGATACACCAGTCACACCTACAGGGTATGCGAGTATTACTGGATTTGATAATCCTAAAGTAGGT
GTTTGCTTAAAACCGAAAGAACAAGGGATGCTTGCATCTAGAGGTGATGATGGTGTTGTGGTGTGTGTAGGCAGTGGTGC
TAATTTGCCACCAGAAAAAGTTATGGATGGTGACTATGCAAACCCTAAAAATATTAAATGTGTTGGTGGATATGCTTATT
ATGTTGGCTCTGCACGCAAAATTTATAAACGTGTGGATATTGGGAAATGGCAGAGAATTCAACATGGGATACCAGGAGGA
GAAACCAATACAGATATGGGGTTCACTGATCTCGATGGTTTTTCTGAGCAGGATATGTATGCCGTTGGCGGTGAAGGCGA
TGTTTGGCATTATAATGGCGAAATCTGGATCCCCTGTGGTTTTCCCTCTAATGAGCAATTAAGTGCAGTTTTATGTGCCC
CTGATGGTTATGTGTATATTGGCGGTGAAGGGGGCAGTTTATGGTGTGGGCGTGAATATCAATGGGAAAAACTTTATCAC
GGCAGTTCGACTATTTTACTGAATCAGTTACGCTGGTTTGAAGATAAAGTGTGGGCATGTGATGACTACCGTTTACAGTG
TTGGAATGGTAAAGAAATGGTCCGTCCCAAGGATGGTGATAAAAATGTTATCGCCTCAGGTCATATGGATGTCCGCGATG
GGATTTTAGTTGTAGCTGATGATTATAATGTTGATCTGTATGATGGAACCGCTTGGCATAAGATAGTGAGACCTTATAAA
TAA

                                