Detailed information of regulator
Summary
| Regulator ID | REG0201  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00272 (Type i1) |
| Strain | Proteus mirabilis HI4320 |
| Replicon | chromosome |
| Sequence | Protein sequence (108 a.a.); Nucleotide sequence (324 bp) |
| Description | MrpJ directly binds and regulates parts of T6SS genes promotor.; |
| Reference | [1] Debnath I, Stringer AM, Smith SN, et al. MrpJ Directly Regulates Proteus mirabilis Virulence Factors, Including Fimbriae and Type VI Secretion, during Urinary Tract Infection. Infect Immun. 2018 Sep 21;86(10). pii: e00388-18. doi: 10.1128/IAI.00388-18. Print 2018 Oct. PMID: 30082479 [2] Bode NJ, Debnath I, Kuan L, et al. Transcriptional analysis of the MrpJ network: modulation of diverse virulence-associated genes and direct regulation of mrp fimbrial and flhDC flagellar operons in Proteus mirabilis. Infect Immun. 2015 Jun;83(6):2542-56. doi: 10.1128/IAI.02978-14. Epub 2015 Apr 6. PMID: 25847961 |
External database links
| Locus tag (Gene) | PMI0271 (MrpJ) |
| Coordinates (Strand) | 318458..318781 (+) |
| NCBI ID | 490365253 |
| RefSeq | NC_010554 |
| Uniprot ID | Q9R6Q7_PROMI |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| PMI0749 (tssA) | ACAAATATAATGAGTA/ACTCTAATAATGAGTA The binding motif of ChIP-enriched MrpJ gene targets |
|
| PMI2990 (idsA/hcp) | ACACTCAATATGCGTA/ACACTTTTTATGACTG The binding motif of ChIP-enriched MrpJ gene targets |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| HTH_3 | PF01381.24 | 1.4e-12 | 20..74 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-107 | MMMNFDFANIDVNASVGKKIQKKRKELGYTGMQLAKKIGVSQQQFSRYERGMNKIDLRHLVLLALYLNTPIYWFFEDCYVKKPSLNNKGIDKRNYVIAQATPDAFHY |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9854 | - | - |
Protein sequence of regulator: 108 a.a. .
>REG0201 NC_010554:318458-318781 [Proteus mirabilis HI4320]
MMMNFDFANIDVNASVGKKIQKKRKELGYTGMQLAKKIGVSQQQFSRYERGMNKIDLRHLVLLALYLNTPIYWFFEDCYV
KKPSLNNKGIDKRNYVIAQATPDAFHY*
MMMNFDFANIDVNASVGKKIQKKRKELGYTGMQLAKKIGVSQQQFSRYERGMNKIDLRHLVLLALYLNTPIYWFFEDCYV
KKPSLNNKGIDKRNYVIAQATPDAFHY*
Nucleotide sequence of regulator: 324 bp .
>REG0201 NC_010554:318458-318781 [Proteus mirabilis HI4320]
ATGATGATGAATTTTGATTTTGCGAATATAGATGTAAATGCTTCTGTAGGTAAAAAGATCCAAAAAAAACGTAAAGAGCT
GGGTTATACCGGTATGCAGCTGGCTAAAAAAATTGGTGTCAGCCAGCAACAGTTTTCTCGCTATGAACGAGGTATGAACA
AAATAGATCTCAGACATTTAGTGTTGTTAGCTCTCTATTTAAATACACCCATTTATTGGTTTTTTGAGGATTGCTACGTA
AAAAAGCCTTCATTAAATAATAAAGGAATAGATAAGCGCAATTATGTTATTGCTCAAGCAACACCTGATGCTTTTCATTA
TTGA
ATGATGATGAATTTTGATTTTGCGAATATAGATGTAAATGCTTCTGTAGGTAAAAAGATCCAAAAAAAACGTAAAGAGCT
GGGTTATACCGGTATGCAGCTGGCTAAAAAAATTGGTGTCAGCCAGCAACAGTTTTCTCGCTATGAACGAGGTATGAACA
AAATAGATCTCAGACATTTAGTGTTGTTAGCTCTCTATTTAAATACACCCATTTATTGGTTTTTTGAGGATTGCTACGTA
AAAAAGCCTTCATTAAATAATAAAGGAATAGATAAGCGCAATTATGTTATTGCTCAAGCAACACCTGATGCTTTTCATTA
TTGA