SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002719
Component protein type	TssL
T6SS ID (Type)	T6SS00272 (Type i1)
Strain	Proteus mirabilis HI4320
Replicon	chromosome
Sequence	Protein sequence (252 a.a.); Nucleotide sequence (756 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PMI_RS03645
Coordinate (Strand)	820659..821414 (-)
NCBI ID	WP_004244617.1
RefSeq	NC_010554
Uniprot ID	B4ET53_PROMH, A0A1Z1SWQ8_PROMI
KEGG ID	pmr:PMI0741, pvl:AOB99_06095
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	7.2e-56	31..232

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-210	MTDDILLNHDDEERITKKNYQLPLRGNNINPMIDAATPLLGMVLRMKAMSETPLSEKLYQQVVMDITSIEQQLQIQGYEPGAIVSFRYVLCTFIDETALGLGWDQDNGWVKQSLLVHFHNESWGGEKVFVLIERLLGEPKRYLDLIEFIYLCLCLGYRGRYKVSAGQNDEFNHLLRRLQKQIQQLRGNAKPIVLFEKGDNQQSRYRLGKR
Transmembrane helix	211-233	LGARYILIGTAVFALIIYSIYSS
Outside	234-251	RLNYQTLSIVEQLNTLLG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9827	-	-

Protein sequence: 252 a.a.

						>T6CP002719 NC_010554:c821414-820659 [Proteus mirabilis HI4320] [TssL]
MTDDILLNHDDEERITKKNYQLPLRGNNINPMIDAATPLLGMVLRMKAMSETPLSEKLYQQVVMDITSIEQQLQIQGYEP
GAIVSFRYVLCTFIDETALGLGWDQDNGWVKQSLLVHFHNESWGGEKVFVLIERLLGEPKRYLDLIEFIYLCLCLGYRGR
YKVSAGQNDEFNHLLRRLQKQIQQLRGNAKPIVLFEKGDNQQSRYRLGKRLGARYILIGTAVFALIIYSIYSSRLNYQTL
SIVEQLNTLLG*					

Nucleotide sequence: 756 bp

						>T6CP002719 NC_010554:c821414-820659 [Proteus mirabilis HI4320] [TssL]
ATGACTGATGATATTTTACTGAATCATGATGATGAAGAGCGCATTACAAAGAAAAATTACCAACTGCCGCTAAGAGGAAA
CAACATCAATCCAATGATAGATGCTGCTACACCTTTATTAGGCATGGTATTGCGCATGAAAGCGATGTCTGAAACCCCAC
TTTCAGAAAAGCTGTACCAACAAGTTGTCATGGATATTACCTCTATTGAGCAACAATTACAGATCCAAGGTTACGAGCCA
GGTGCCATTGTTTCATTTCGTTATGTGCTATGTACTTTTATTGATGAAACCGCCCTTGGATTAGGCTGGGATCAAGATAA
TGGTTGGGTAAAACAATCATTACTGGTTCATTTTCATAATGAATCATGGGGTGGTGAAAAAGTCTTTGTCTTAATTGAAA
GATTATTAGGTGAACCTAAGCGTTACCTCGATCTGATTGAATTTATCTACCTTTGTTTATGCCTAGGATACCGCGGGCGC
TACAAAGTCTCTGCGGGACAAAATGATGAATTTAATCATTTATTAAGACGCTTACAAAAACAGATCCAACAGCTAAGAGG
TAACGCTAAGCCAATAGTTCTATTTGAAAAAGGTGATAACCAACAAAGCCGTTATCGTTTAGGTAAGCGTTTAGGGGCTC
GCTATATTCTCATAGGTACAGCGGTATTCGCGTTGATTATTTATAGCATTTATTCATCCAGACTGAATTATCAAACACTC
AGTATTGTGGAACAACTTAATACTTTACTTGGCTAG					