SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002723
Component protein type	TssF
T6SS ID (Type)	T6SS00272 (Type i1)
Strain	Proteus mirabilis HI4320
Replicon	chromosome
Sequence	Protein sequence (592 a.a.); Nucleotide sequence (1776 bp)
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014 [2] Alteri CJ, Himpsl SD, Pickens SR, et al. Multicellular bacteria deploy the type VI secretion system to preemptively strike neighboring cells. PLoS Pathog. 2013;9(9):e1003608. doi: 10.1371/journal.ppat.1003608. Epub 2013 Sep 5. PMID: 24039579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PMI_RS03670
Coordinate (Strand)	825594..827369 (-)
NCBI ID	WP_012367723.1
RefSeq	NC_010554
Uniprot ID	B4ET58_PROMH
KEGG ID	pmr:PMI0746
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	8.8e-119	5..586

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-591	MSSKSYFTNESEYLNELTEQVAKEKPQLVNLLSHNVKDPDTSRIMDGLSYLSGNLRQQIDRQFPELTNSLANMLWPNYARPVPSMTIVEYHPNYAQCQHATKISAGQPFSAAPLYVQSEETNHETLFQCRFSQSRDLWLMPTKISNILQQTTAIEITFELATKQPLANIGLDKLCFYLNGAPFTTNQLYFLLSQHVQSASLVTDVHTLPLTHFSVTPVGFQKQDALLPYPKNSYDGYRILQEFFCFPEAFLFLDIHGLDDIPLAIEAEQFKLVINFDLEIPDGVILYDDSIKLNCTPIVNLFPIDSEAINLTGKSEEYVLSPNYQLTECFDIFSINEVRGLRYPNDAKPYPITYTAFESFYHQNHQTQNHGYYRLKISQNEQLYGYSHRLSFVRQQEPQLQQCEETVSVALNCTNRYIASQLSTRSIDLDSASMPYGALSAKNLFAPTLPLYPLLDKTLYWSELTNLSLNYQSLLNLDSLKKILQLYDLPSVYNTQVARQSQKRLDALVDLHSEHIEHIYKGLPVRGLKTTLVVHQNAFLSEGSLFLFCTVIAQFFTLYTSINMFHELEVINLDNKEVYRWPAQINQHILK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9931	-	-

Protein sequence: 592 a.a.

						>T6CP002723 NC_010554:c827369-825594 [Proteus mirabilis HI4320] [TssF]
MSSKSYFTNESEYLNELTEQVAKEKPQLVNLLSHNVKDPDTSRIMDGLSYLSGNLRQQIDRQFPELTNSLANMLWPNYAR
PVPSMTIVEYHPNYAQCQHATKISAGQPFSAAPLYVQSEETNHETLFQCRFSQSRDLWLMPTKISNILQQTTAIEITFEL
ATKQPLANIGLDKLCFYLNGAPFTTNQLYFLLSQHVQSASLVTDVHTLPLTHFSVTPVGFQKQDALLPYPKNSYDGYRIL
QEFFCFPEAFLFLDIHGLDDIPLAIEAEQFKLVINFDLEIPDGVILYDDSIKLNCTPIVNLFPIDSEAINLTGKSEEYVL
SPNYQLTECFDIFSINEVRGLRYPNDAKPYPITYTAFESFYHQNHQTQNHGYYRLKISQNEQLYGYSHRLSFVRQQEPQL
QQCEETVSVALNCTNRYIASQLSTRSIDLDSASMPYGALSAKNLFAPTLPLYPLLDKTLYWSELTNLSLNYQSLLNLDSL
KKILQLYDLPSVYNTQVARQSQKRLDALVDLHSEHIEHIYKGLPVRGLKTTLVVHQNAFLSEGSLFLFCTVIAQFFTLYT
SINMFHELEVINLDNKEVYRWPAQINQHILK*					

Nucleotide sequence: 1776 bp

						>T6CP002723 NC_010554:c827369-825594 [Proteus mirabilis HI4320] [TssF]
ATGTCATCAAAATCCTACTTTACCAATGAATCAGAATACTTAAATGAGCTGACTGAACAAGTTGCCAAAGAAAAACCGCA
ACTTGTTAATTTGCTTTCGCACAATGTTAAAGATCCTGATACTTCGCGGATTATGGATGGGCTATCTTATTTATCGGGTA
ATTTACGCCAGCAAATAGATAGACAATTTCCTGAATTAACTAATAGTTTAGCCAATATGCTTTGGCCAAACTACGCACGC
CCTGTGCCAAGCATGACGATTGTTGAATATCATCCTAATTATGCTCAGTGCCAACACGCAACAAAAATTTCAGCAGGACA
ACCCTTTAGTGCCGCTCCACTTTATGTGCAAAGTGAGGAAACAAACCACGAGACGTTATTTCAATGTCGATTTAGTCAAA
GTCGCGACCTCTGGTTAATGCCGACAAAAATCAGTAACATTTTGCAGCAAACAACCGCCATTGAAATAACATTTGAGTTA
GCAACAAAACAGCCACTTGCCAATATTGGCTTAGATAAATTGTGTTTTTACTTAAATGGTGCGCCATTTACTACAAATCA
GCTCTATTTTTTATTAAGCCAGCATGTGCAGAGTGCCTCTTTAGTCACAGATGTACATACGCTACCTCTTACTCACTTTT
CAGTTACGCCTGTTGGTTTTCAAAAGCAGGATGCCTTACTTCCATACCCGAAAAATAGCTATGATGGCTATCGTATACTG
CAAGAGTTTTTCTGTTTTCCTGAAGCCTTTCTCTTTCTAGATATTCATGGTTTGGATGACATTCCTTTGGCTATCGAAGC
AGAGCAATTCAAACTGGTGATCAATTTTGATCTAGAAATTCCTGATGGCGTTATTCTTTACGACGACAGTATTAAACTTA
ATTGCACCCCTATTGTGAATTTATTTCCTATTGATAGTGAAGCAATAAATTTGACGGGGAAAAGTGAGGAGTATGTTTTA
AGTCCTAATTACCAACTTACTGAATGTTTTGATATTTTTTCGATTAATGAGGTTAGAGGGTTACGTTATCCTAATGATGC
TAAACCTTATCCGATCACCTATACAGCATTTGAAAGTTTTTATCATCAAAACCATCAGACTCAAAATCATGGCTATTACC
GTTTAAAGATAAGTCAAAATGAACAGTTATACGGCTATTCCCATCGACTTTCTTTTGTTCGTCAGCAAGAGCCTCAGTTA
CAACAGTGTGAAGAAACCGTTTCTGTTGCACTCAACTGTACTAACCGCTATATCGCTTCCCAGTTATCGACGCGTTCTAT
CGATTTAGATAGTGCATCAATGCCTTATGGGGCTTTATCTGCTAAGAATTTATTCGCACCAACACTGCCTTTATATCCAT
TATTAGATAAAACATTATATTGGTCTGAATTAACTAATTTATCTTTAAATTATCAGTCTTTGCTCAATCTTGATTCATTA
AAAAAAATATTACAACTCTATGATTTACCGTCTGTTTATAACACACAGGTTGCAAGGCAATCACAAAAACGCCTAGATGC
ATTAGTTGATTTACATTCAGAGCATATTGAGCATATCTACAAAGGCCTTCCTGTTCGCGGTCTGAAGACAACGTTAGTTG
TTCATCAAAATGCGTTTCTTTCAGAAGGAAGTTTATTCCTATTTTGTACCGTTATCGCTCAATTTTTCACCTTATATACC
AGCATTAATATGTTTCATGAGTTGGAAGTTATTAACCTTGATAATAAGGAAGTTTATCGATGGCCAGCACAAATCAACCA
GCACATACTGAAGTAA					