Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00113 experimental
T6SS type Type i1
Strain Burkholderia thailandensis E264
Replicon (RefSeq) chromosome II (NC_007650)
Location 996276..1025886
Function anti-eukaryotic; virulence
Comment Virulence in mouse acute pneumonia model of melioidosis; mononuclear giant cell formation and intercellular spread in cultured cells.
References
[1] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806]
[2] Schwarz S et al (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170]
[3] Fisher NA et al (2012) The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 0.58125. [PudMed:22892068]
[4] Schwarz S et al (2014) VgrG-5 is a Burkholderia type VI secretion exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 82(4):1445-52. [PudMed:24452686]
[5] Schwarz S et al (2014) VgrG-5 is a Burkholderia type VI secretion exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 82(4):1445-52. [PudMed:24452686]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssMVirAVirG
Number111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
BTH_RS04415 (sicP) 992960..993415 + 456 WP_009896165.1 chaperone SicP
BTH_RS04420 (bprB) 993650..994321 + 672 WP_011401051.1 two-component system response regulator BprB
BTH_RS04425 994546..994989 + 444 WP_009896167.1 hypothetical protein
BTH_RS04430 995224..996285 + 1062 WP_009896168.1 helix-turn-helix domain-containing protein
BTH_RS04435 996276..997382 - 1107 WP_025370194.1 Ig-like domain-containing protein
T6CP001062 TssM BTH_RS04440 997685..1001575 - 3891 WP_009896173.1 type VI secretion system protein ImpL
T6CP001063 TssL BTH_RS04445 1001572..1002249 - 678 WP_025370195.1 DotU family type IV/VI secretion system protein
T6CP001064 TssK BTH_RS04450 (tssK) 1002249..1003643 - 1395 WP_009896176.1 type VI secretion system baseplate subunit TssK
T6CP001065 TssJ BTH_RS04455 (tssJ) 1003676..1004482 - 807 WP_011401053.1 type VI secretion system lipoprotein TssJ
BTH_RS04460 1004492..1004884 - 393 WP_009896180.1 DUF4150 domain-containing protein
BTH_RS04465 1004913..1005689 - 777 WP_039339719.1 DUF3540 domain-containing protein
BTH_RS04470 1005686..1006750 - 1065 WP_009896184.1 pentapeptide repeat-containing protein
BTH_RS04475 1006768..1009386 - 2619 WP_009896186.1 pentapeptide repeat-containing protein
T6CP001066 TssI BTH_RS04480 (tssI) 1009425..1012463 - 3039 WP_009896187.1 type VI secretion system tip protein VgrG
T6CP001067 TssH BTH_RS04485 (tssH) 1012489..1015365 - 2877 WP_009896189.1 type VI secretion system ATPase TssH
T6CP001068 TssG BTH_RS04490 (tssG) 1015352..1016383 - 1032 WP_009896191.1 type VI secretion system baseplate subunit TssG
T6CP001069 TssF BTH_RS04495 (tssF) 1016371..1018113 - 1743 WP_009896193.1 type VI secretion system baseplate subunit TssF
T6CP008465 TssE BTH_RS04500 (tssE) 1018142..1018618 - 477 WP_009896194.1 type VI secretion system baseplate subunit TssE
T6CP008466 TssD BTH_RS04505 1018597..1019085 - 489 WP_009896195.1 Hcp family type VI secretion system effector
T6CP001070 TssC BTH_RS04510 (tssC) 1019268..1020794 - 1527 WP_009896197.1 type VI secretion system contractile sheath large subunit
T6CP001071 TssB BTH_RS04515 (tssB) 1020791..1021285 - 495 WP_009896198.1 type VI secretion system contractile sheath small subunit
T6CP012448 VirA BTH_RS04520 1021545..1023368 + 1824 WP_009896199.1 MASE1 domain-containing protein
T6CP012449 VirG BTH_RS04525 1023365..1024099 + 735 WP_009907461.1 response regulator transcription factor
T6CP008467 TssA BTH_RS04530 (tssA) 1024090..1025886 + 1797 WP_009896202.1 type VI secretion system protein TssA
BTH_RS04535 1026177..1026533 - 357 WP_025370201.1 hypothetical protein
BTH_RS04540 1026584..1027147 - 564 WP_009896205.1 YadA-like family protein
BTH_RS29795 1027413..1027817 + 405 WP_009896206.1 hypothetical protein
BTH_RS04545 1028324..1029649 - 1326 WP_025370202.1 autotransporter strand-loop-strand O-heptosyltransferase
BTH_RS04550 1029806..1030831 - 1026 WP_009896209.1 N-acetylmuramoyl-L-alanine amidase
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0153 experimental BTH_I1281 (OxyR) protein Global regulator Burkholderia thailandensis E264 chromosome (NC_007651) 1432632..1433591 (+) 497575020 BTH_RS09735 (T6SS-4) Promotor,−536 to −186 relative to the ATG start codon (↑)
REG0154 experimental bth_I0691 (Zur) protein Global regulator Burkholderia thailandensis E264 chromosome (NC_007651) 796074..796538 (-) 497578547 BTH_RS09735 (T6SS-4) Promotor,−541 to −1 relative to the ATG start codon (↑)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01464 experimental BTH_II1883 (TseM) Tie - Burkholderia thailandensis E264 chromosome (NC_007650) 2281342..2281767 (-) 83718045
EFF01825 experimental BTH_II1884 (TseZ) Tie - Burkholderia thailandensis E264 chromosome (NC_007650) 2281790..2282149 (-) 497579572
EFF00138 experimental BTH_RS04480 - - Burkholderia thailandensis E264 chromosome (NC_007650) 1009425..1012463 (-) 83717090
EFF01271 experimental BTH_RS04505 - - Burkholderia thailandensis E264 chromosome (NC_007650) 1018597..1019085 (-) 83716550

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00021 experimental BTH_II2139 (hmuR) chaperone Burkholderia thailandensis E264 chromosome (NC_007650) 2626542..2628827 (+) 644827063 - BTH_II1884 (TseZ)