Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00113  |
| T6SS type | Type i1 |
| Strain | Burkholderia thailandensis E264 |
| Replicon (RefSeq) | chromosome II (NC_007650) |
| Location | 996276..1025886 |
| Function | anti-eukaryotic; virulence |
| Comment | Disruption of the T6SS-5 in B. thailandensis causes a drastic attenuation of virulence in wildtype but not in mice lacking the central innate immune adapter protein MyD88. This result suggests that the T6SS-5 is deployed by the bacteria to overcome innate immune responses. |
| References | [1] Lennings J, Mayer C, Makhlouf M, Brötz-Oesterhelt H, Schwarz S. et al (2019) Polar localization of the ATPase ClpV-5 occurs independent of type VI secretion system apparatus proteins in Burkholderia thailandensis.. BMC Res Notes. 12(1):109. [PudMed:30819219] [2] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] [3] Fisher NA et al (2012) The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 0.58125. [PudMed:22892068] [4] Schwarz S et al (2014) VgrG-5 is a Burkholderia type VI secretion exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 82(4):1445-52. [PudMed:24452686] [5] Lennings J, West TE, Schwarz S. et al (2019) The Burkholderia Type VI Secretion System 5: Composition, Regulation and Role in Virulence.. Front Microbiol. 2.69375. [PudMed:30687298] [6] Schwarz S et al (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170] [7] Lennings J, Makhlouf M, Olejnik P, Mayer C, Brötz-Oesterhelt H, Schwarz S. et al (2019) Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis.. Int J Med Microbiol. 309(6):151335. [PudMed:31378704] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | VirA | VirG |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BTH_RS04415 (sicP) | 992960..993415 | + | 456 | WP_009896165.1 | chaperone SicP | ||
| BTH_RS04420 (bprB) | 993650..994321 | + | 672 | WP_011401051.1 | two-component system response regulator BprB | ||
| BTH_RS04425 | 994546..994989 | + | 444 | WP_009896167.1 | hypothetical protein | ||
| BTH_RS04430 | 995224..996285 | + | 1062 | WP_009896168.1 | helix-turn-helix domain-containing protein | ||
| BTH_RS04435 | 996276..997382 | - | 1107 | WP_025370194.1 | Ig-like domain-containing protein | ||
| T6CP001062 | TssM | BTH_RS04440 | 997685..1001575 | - | 3891 | WP_009896173.1 | type VI secretion system protein ImpL |
| T6CP001063 | TssL | BTH_RS04445 | 1001572..1002249 | - | 678 | WP_025370195.1 | DotU family type IV/VI secretion system protein |
| T6CP001064 | TssK | BTH_RS04450 (tssK) | 1002249..1003643 | - | 1395 | WP_009896176.1 | type VI secretion system baseplate subunit TssK |
| T6CP001065 | TssJ | BTH_RS04455 (tssJ) | 1003676..1004482 | - | 807 | WP_011401053.1 | type VI secretion system lipoprotein TssJ |
| BTH_RS04460 | 1004492..1004884 | - | 393 | WP_009896180.1 | DUF4150 domain-containing protein | ||
| BTH_RS04465 | 1004913..1005689 | - | 777 | WP_039339719.1 | DUF3540 domain-containing protein | ||
| BTH_RS04470 | 1005686..1006750 | - | 1065 | WP_009896184.1 | pentapeptide repeat-containing protein | ||
| BTH_RS04475 | 1006768..1009386 | - | 2619 | WP_009896186.1 | pentapeptide repeat-containing protein | ||
| T6CP001066 | TssI | BTH_RS04480 (tssI) | 1009425..1012463 | - | 3039 | WP_009896187.1 | type VI secretion system tip protein VgrG |
| T6CP001067 | TssH | BTH_RS04485 (tssH) | 1012489..1015365 | - | 2877 | WP_009896189.1 | type VI secretion system ATPase TssH |
| T6CP001068 | TssG | BTH_RS04490 (tssG) | 1015352..1016383 | - | 1032 | WP_009896191.1 | type VI secretion system baseplate subunit TssG |
| T6CP001069 | TssF | BTH_RS04495 (tssF) | 1016371..1018113 | - | 1743 | WP_009896193.1 | type VI secretion system baseplate subunit TssF |
| T6CP008465 | TssE | BTH_RS04500 (tssE) | 1018142..1018618 | - | 477 | WP_009896194.1 | type VI secretion system baseplate subunit TssE |
| T6CP008466 | TssD | BTH_RS04505 | 1018597..1019085 | - | 489 | WP_009896195.1 | Hcp family type VI secretion system effector |
| T6CP001070 | TssC | BTH_RS04510 (tssC) | 1019268..1020794 | - | 1527 | WP_009896197.1 | type VI secretion system contractile sheath large subunit |
| T6CP001071 | TssB | BTH_RS04515 (tssB) | 1020791..1021285 | - | 495 | WP_009896198.1 | type VI secretion system contractile sheath small subunit |
| T6CP012448 | VirA | BTH_RS04520 | 1021545..1023368 | + | 1824 | WP_009896199.1 | MASE1 domain-containing protein |
| T6CP012449 | VirG | BTH_RS04525 | 1023365..1024099 | + | 735 | WP_009907461.1 | response regulator transcription factor |
| T6CP008467 | TssA | BTH_RS04530 (tssA) | 1024090..1025886 | + | 1797 | WP_009896202.1 | type VI secretion system protein TssA |
| BTH_RS04535 | 1026177..1026533 | - | 357 | WP_025370201.1 | hypothetical protein | ||
| BTH_RS04540 | 1026584..1027147 | - | 564 | WP_009896205.1 | YadA-like family protein | ||
| BTH_RS29795 | 1027413..1027817 | + | 405 | WP_009896206.1 | hypothetical protein | ||
| BTH_RS04545 | 1028324..1029649 | - | 1326 | WP_025370202.1 | autotransporter strand-loop-strand O-heptosyltransferase | ||
| BTH_RS04550 | 1029806..1030831 | - | 1026 | WP_009896209.1 | N-acetylmuramoyl-L-alanine amidase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF00138 |
BTH_RS04480 | - | - | Burkholderia thailandensis E264 | chromosome (NC_007650) | 1009425..1012463 (-) | 83717090 |
| EFF01271 |
BTH_RS04505 | - | - | Burkholderia thailandensis E264 | chromosome (NC_007650) | 1018597..1019085 (-) | 83716550 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|