SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00138
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00113 (Type i1)
Strain	Burkholderia thailandensis E264
Replicon	chromosome
Sequence	Protein sequence (1013 a.a.); Nucleotide sequence (3039 bp)
Description	-
Reference	[1] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BTH_RS04480
Coordinates (Strand)	1009425..1012463 (-)
NCBI ID	83717090
RefSeq	NC_007650
Uniprot ID	UPI000066765E
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	1.2e-70	58..361
Phage_base_V	PF04717.14	1e-06	426..492

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1012	MSSSHRHYADTALADVAALTDAASRADAAPLANARRFTFASTAYDAATFDVVDIDGRDAISQPYRFEITLVSRSVRIDFAKMLSCEATLAILPPFGEAGTTRYAGVLAEFEQKERFRDFTVYRAALVPRLWRLSLYKASDVYLNEQTIPDIVKRVLRAASFGKRNFRMRHRGVYRKRSFVCQYDESHLDFVSRWMEKEGLYYYFEHDGRREKLEIVDDRRDQPGPADDLALRYLPATCLDAGIESDRVQAFACRATPLPREVVLRDFNHRKAELSLEVREHVAHDGVGERVSSDEHFHTKDEGRRYAKLRAEALVCEGRRFAGESTAAGLRAGRFFALSGHYRKDFDGRYLVTAVTHRGSQAHLLFPDLDAPFGATPGEPVYRAEFEAIAANLQYRPPRTTPKPRAAGVVSAIVDGEGSGKRAELDEHGQYKVRFPFAHTAHPTNKASARIRMATPYAGDDRGMHLPLLKRTEVKIAFDGGDPDRPVIVGAVPNSSHRSVVTRSNPDAHRILTEHNQLYMKDGSGAATWLHAPNNHIGIGAVGPGDGLALLTSGNKFDFSLGNAYSFSGGLKCSVSMGGNTDIYVGVRNSLDVSANFLTTLQGNLRWMLPGSRSFEINDSASTLLQTLHKQSATGAIRLSAGQDASALLQKQLDKLKGTVRKFMIVSGLANAGAAATAAGLIKGGGALADLPWAGFGVSAAQFAGATGFSTALMATSRTLLSKIAKLQEALPLVADLSLDKQGIALAAKNLTHATRMSLTVDGVSWSTHAKGPGAAGAAMSVGKGRWGVEAAEHAHVHANDTLLFAVPADPTSKFDLKELIGLRRDLDECVKGIADLEADISENEVLSTDQNTFGVGALVPTPPSPANAVAAVAIKAKEAKLVELNAKRKLVATKIDNLQQKLAKHAKNLSAARMSASDAEVGFKGNRLVATAEGVTLAHAQGKAKLDVREAKIGVEAGKSSLELDESKLAAGCGGASLKLGSDGAIDVRATNVKLNGSASLKLDGQLIQLG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8709	-	-

Protein sequence: 1013 a.a. .

                                    >EFF00138 NC_007650:c1012463-1009425  (Unknown) [Burkholderia thailandensis E264]
MSSSHRHYADTALADVAALTDAASRADAAPLANARRFTFASTAYDAATFDVVDIDGRDAISQPYRFEITLVSRSVRIDFA
KMLSCEATLAILPPFGEAGTTRYAGVLAEFEQKERFRDFTVYRAALVPRLWRLSLYKASDVYLNEQTIPDIVKRVLRAAS
FGKRNFRMRHRGVYRKRSFVCQYDESHLDFVSRWMEKEGLYYYFEHDGRREKLEIVDDRRDQPGPADDLALRYLPATCLD
AGIESDRVQAFACRATPLPREVVLRDFNHRKAELSLEVREHVAHDGVGERVSSDEHFHTKDEGRRYAKLRAEALVCEGRR
FAGESTAAGLRAGRFFALSGHYRKDFDGRYLVTAVTHRGSQAHLLFPDLDAPFGATPGEPVYRAEFEAIAANLQYRPPRT
TPKPRAAGVVSAIVDGEGSGKRAELDEHGQYKVRFPFAHTAHPTNKASARIRMATPYAGDDRGMHLPLLKRTEVKIAFDG
GDPDRPVIVGAVPNSSHRSVVTRSNPDAHRILTEHNQLYMKDGSGAATWLHAPNNHIGIGAVGPGDGLALLTSGNKFDFS
LGNAYSFSGGLKCSVSMGGNTDIYVGVRNSLDVSANFLTTLQGNLRWMLPGSRSFEINDSASTLLQTLHKQSATGAIRLS
AGQDASALLQKQLDKLKGTVRKFMIVSGLANAGAAATAAGLIKGGGALADLPWAGFGVSAAQFAGATGFSTALMATSRTL
LSKIAKLQEALPLVADLSLDKQGIALAAKNLTHATRMSLTVDGVSWSTHAKGPGAAGAAMSVGKGRWGVEAAEHAHVHAN
DTLLFAVPADPTSKFDLKELIGLRRDLDECVKGIADLEADISENEVLSTDQNTFGVGALVPTPPSPANAVAAVAIKAKEA
KLVELNAKRKLVATKIDNLQQKLAKHAKNLSAARMSASDAEVGFKGNRLVATAEGVTLAHAQGKAKLDVREAKIGVEAGK
SSLELDESKLAAGCGGASLKLGSDGAIDVRATNVKLNGSASLKLDGQLIQLG*

                                

Nucleotide sequence: 3039 bp .

                                    >EFF00138 NC_007650:c1012463-1009425  (Unknown) [Burkholderia thailandensis E264]
ATGTCTTCGTCCCATCGACACTACGCCGACACGGCGCTCGCGGATGTCGCGGCGCTCACGGATGCCGCGTCGCGCGCCGA
CGCCGCGCCGCTCGCGAACGCGCGACGCTTCACGTTCGCGAGCACCGCGTACGACGCTGCCACGTTCGACGTCGTCGACA
TCGACGGCCGCGACGCGATCTCGCAGCCGTACCGGTTCGAGATCACGCTCGTGAGCAGGAGTGTGCGGATCGACTTCGCG
AAGATGCTGAGTTGCGAGGCGACGCTCGCGATCCTGCCGCCGTTCGGTGAGGCCGGCACGACCCGCTATGCCGGCGTGCT
CGCCGAATTCGAGCAGAAGGAACGCTTTCGCGACTTCACCGTCTATCGCGCGGCGCTCGTGCCGCGCCTCTGGCGCCTCT
CGCTGTACAAGGCGTCGGACGTCTACCTGAACGAGCAGACGATTCCCGATATCGTCAAGCGCGTGCTGCGCGCCGCGTCG
TTCGGCAAGCGCAATTTCCGCATGCGGCACCGCGGCGTCTACCGCAAGCGCAGCTTCGTCTGCCAGTACGACGAGAGCCA
TCTCGATTTCGTGTCGCGCTGGATGGAGAAGGAAGGCCTCTACTACTACTTCGAGCATGACGGCCGACGCGAAAAGCTCG
AGATCGTTGACGACCGCCGCGACCAGCCCGGCCCCGCCGACGATCTCGCGCTGCGCTACCTACCCGCCACCTGTCTCGAC
GCGGGCATCGAATCGGACCGCGTGCAGGCGTTCGCATGCCGCGCGACGCCGCTGCCGCGCGAGGTCGTGCTGCGCGATTT
CAACCACCGCAAGGCGGAGCTGTCGCTCGAAGTCCGCGAGCACGTGGCGCACGACGGCGTCGGCGAGCGGGTATCGAGCG
ACGAGCATTTCCACACGAAGGACGAAGGGCGGCGCTACGCGAAGCTGCGCGCCGAGGCGCTCGTTTGCGAAGGCCGCCGT
TTCGCCGGCGAATCGACCGCGGCCGGGCTGCGCGCGGGCCGCTTCTTCGCGCTCTCGGGCCACTACCGCAAGGACTTCGA
CGGCCGCTATCTGGTGACGGCGGTCACGCATCGCGGCTCGCAGGCGCACCTGCTGTTTCCCGATCTCGACGCGCCGTTCG
GCGCGACGCCGGGCGAGCCCGTCTACCGCGCCGAGTTCGAGGCGATCGCCGCCAACCTCCAGTACCGGCCGCCGCGCACG
ACGCCGAAGCCGCGCGCGGCGGGCGTCGTCAGCGCGATCGTCGACGGCGAGGGCAGCGGCAAGCGCGCCGAACTCGACGA
ACACGGCCAGTACAAAGTGCGCTTTCCGTTCGCGCACACCGCGCATCCGACGAACAAGGCCTCCGCGCGCATCCGGATGG
CGACGCCCTATGCGGGCGACGACCGCGGCATGCATCTGCCGCTTCTGAAGCGCACCGAAGTGAAGATCGCATTCGACGGC
GGCGATCCGGACCGCCCCGTGATCGTCGGCGCGGTGCCCAACTCGTCGCACCGCAGCGTCGTCACGCGCAGCAACCCCGA
CGCGCACCGGATCCTCACCGAGCACAACCAGCTCTACATGAAGGACGGCAGCGGCGCGGCGACGTGGCTGCACGCGCCGA
ACAACCACATCGGCATCGGCGCGGTCGGGCCGGGCGACGGCCTCGCGCTCCTCACGTCCGGCAACAAGTTCGATTTCTCG
CTCGGCAACGCGTACAGCTTCTCGGGCGGGCTCAAGTGCTCGGTGTCGATGGGCGGCAACACCGACATCTACGTCGGCGT
GCGCAACAGCCTCGACGTCAGCGCGAACTTCCTGACGACGCTGCAGGGCAACCTGCGCTGGATGCTGCCCGGCAGCCGAA
GCTTCGAGATCAACGACAGCGCGTCCACGCTGCTGCAGACGCTGCACAAGCAGTCCGCGACGGGCGCGATCCGGCTGTCC
GCCGGGCAGGACGCGTCCGCGCTGCTGCAAAAGCAGCTCGACAAGCTCAAGGGCACGGTGCGCAAGTTCATGATCGTGTC
GGGCCTCGCGAACGCCGGAGCCGCGGCCACCGCTGCGGGGCTCATCAAGGGCGGCGGCGCGCTCGCCGATCTGCCGTGGG
CGGGCTTCGGCGTGTCCGCCGCGCAGTTCGCCGGCGCGACCGGCTTCAGCACGGCGCTGATGGCGACCTCGCGCACGCTG
CTCTCGAAAATCGCGAAGCTCCAGGAGGCGTTGCCGCTCGTCGCCGATCTATCGCTCGACAAGCAAGGCATCGCGCTCGC
GGCGAAGAACCTCACGCACGCGACGCGGATGTCGCTCACCGTCGACGGCGTCTCGTGGTCGACGCACGCGAAGGGGCCGG
GCGCGGCAGGCGCCGCGATGAGCGTCGGCAAGGGCCGCTGGGGCGTCGAAGCGGCGGAGCACGCGCATGTCCACGCGAAT
GACACGCTGCTGTTCGCCGTGCCGGCCGACCCAACGAGCAAGTTCGACCTCAAGGAGCTGATCGGGCTGCGCCGCGATCT
CGACGAATGCGTGAAGGGTATCGCCGATCTCGAAGCCGACATTTCGGAAAACGAAGTGCTTTCGACCGATCAGAACACGT
TCGGCGTCGGCGCGCTCGTGCCCACGCCGCCGTCGCCCGCCAATGCGGTCGCGGCGGTCGCGATCAAGGCGAAGGAAGCG
AAGCTCGTCGAGCTGAACGCCAAACGCAAGCTCGTCGCGACGAAGATCGACAACCTCCAGCAGAAGCTCGCGAAGCACGC
GAAGAACCTGAGCGCCGCGCGGATGAGCGCGTCGGACGCGGAAGTCGGCTTCAAGGGCAACCGGCTCGTCGCGACGGCCG
AAGGCGTCACGCTCGCGCATGCGCAGGGCAAGGCGAAGCTCGACGTGCGCGAGGCGAAGATCGGCGTCGAGGCAGGCAAA
TCGAGCCTCGAGCTCGACGAGAGCAAGCTCGCGGCCGGCTGCGGCGGCGCCTCGCTGAAGCTGGGCAGCGACGGCGCGAT
CGACGTGCGCGCGACTAACGTCAAGCTGAACGGCAGCGCGTCGCTGAAGCTCGACGGACAGTTGATCCAGCTAGGCTGA

                                