Detailed information of component protein


Summary

Component ID T6CP012449
Component protein type VirG
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (245 a.a.); Nucleotide sequence (735 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04525
Coordinate (Strand) 1023365..1024099 (+)
NCBI ID WP_009907461.1
RefSeq NC_007650
Uniprot ID A0A1B4K3E4_BURTH, Q2T6Y0_BURTA
KEGG ID btha:DR62_3411, bte:BTH_II0872
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 5.3e-20 9..117
Trans_reg_C PF00486.30 1.4e-19 161..234

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-244 MNATRSPAVLVVEDDEPLRRVLCNYLTGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRDSQRFPLICVTARGEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSAAPVRRGRHPRAYRFDGWTLNMNTRRLSTSDARDIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKNPKHPELIRTERGVGYYFAAKDVETLWE



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8771 - -



  Protein sequence: 245 a.a.    

>T6CP012449 NC_007650:1023365-1024099 [Burkholderia thailandensis E264] [VirG]
MNATRSPAVLVVEDDEPLRRVLCNYLTGHGMAVDGVGTAAAAAESVGRREYEMVLLDLGLPDGDGIDVLLRWRDSQRFPL
ICVTARGEEADRIMGLEFGADDYVVKPYSLREVLARMRALWRRAEPSAAPVRRGRHPRAYRFDGWTLNMNTRRLSTSDAR
DIPLTVGEFNLLAFFLGSPSRVVTRAQLLECTRAFDDVFDRAVDVQIWRLRKKIEKNPKHPELIRTERGVGYYFAAKDVE
TLWE*

  Nucleotide sequence: 735 bp    

>T6CP012449 NC_007650:1023365-1024099 [Burkholderia thailandensis E264] [VirG]
ATGAATGCGACAAGATCGCCGGCGGTGCTCGTGGTCGAGGACGACGAGCCGTTGCGGCGCGTGCTCTGCAATTACTTGAC
GGGCCACGGCATGGCCGTGGACGGCGTGGGGACGGCGGCCGCGGCTGCCGAATCGGTCGGGCGGCGCGAATACGAAATGG
TGCTGCTCGATCTCGGCCTGCCGGATGGAGACGGCATCGACGTGCTGTTGCGCTGGCGCGATTCGCAGCGTTTTCCGCTT
ATCTGCGTGACGGCCCGAGGCGAGGAGGCCGATCGCATCATGGGCCTCGAATTCGGCGCGGACGATTACGTCGTGAAGCC
GTATTCGCTGCGCGAGGTGCTTGCGCGGATGCGCGCGCTGTGGCGGCGCGCCGAGCCGTCCGCCGCGCCGGTGCGGCGCG
GCAGGCATCCGCGCGCGTACCGTTTCGACGGCTGGACGCTGAACATGAACACGCGTCGGCTGTCGACGAGCGACGCGCGG
GACATTCCGCTCACGGTCGGCGAATTCAATCTGCTGGCGTTCTTTCTCGGCTCGCCGTCGCGCGTCGTCACGCGCGCGCA
GTTGCTCGAATGCACACGCGCGTTCGACGACGTGTTCGACCGCGCGGTCGACGTGCAGATCTGGCGATTGCGCAAGAAGA
TCGAGAAGAATCCGAAGCACCCCGAACTGATTCGAACCGAGCGCGGCGTCGGCTATTACTTCGCCGCGAAGGACGTGGAG
ACGCTATGGGAATGA