Detailed information of component protein


Summary

Component ID T6CP001070
Component protein type TssC
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (509 a.a.); Nucleotide sequence (1527 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04510
Coordinate (Strand) 1019268..1020794 (-)
NCBI ID WP_009896197.1
RefSeq NC_007650
Uniprot ID UPI0000667533
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 8.3e-126 82..381
VipB_2 PF18945.2 7.4e-45 395..505

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-508 MNRDTERTTMEGEHLYSPKNDDAPPAPAPDSPASLLDELIEAARVKRDEEAYPITRHGIEAFVAHLARPKRPIETVSQATIDDMIAEIDRKLCRQVDAILHHPDFQQLESTWRSLKFLVERTDFRENIKIQFLDVGKAALLDDFDDSPDITKSGLYQKVYAAEYGQFGGQPIGAIVANYTFGPGAQDVKLLQYVASTSAMAHTPFIAAAGPAFFGIDSFGKLPNVKDLASLFEGPQFAKWNAFRESEDARYVGLTLPRFLLRLPYGANTTPVKRFNYDERVDGGDADFLWGNAAFAFATRLTASFADYRWCANVIGPKGGGTVADLPLYAYEAMGEIQNKIPTDVLISERREFELAEQGFIALTMRKHSDNAAFFSANSTQKPKFFGISKEGKDAELNYRLGTQLPYIFVVNRLAHYIKVIQRENIGTWKERGDLEQELNQWIRQYVADMDNPTEGVRSRRPLRQAEIFVSDVEGEPGWYRVDMKVRPHFKYMGASFTLSLVGKLEKR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9799 - -



  Protein sequence: 509 a.a.    

>T6CP001070 NC_007650:c1020794-1019268 [Burkholderia thailandensis E264] [TssC]
MNRDTERTTMEGEHLYSPKNDDAPPAPAPDSPASLLDELIEAARVKRDEEAYPITRHGIEAFVAHLARPKRPIETVSQAT
IDDMIAEIDRKLCRQVDAILHHPDFQQLESTWRSLKFLVERTDFRENIKIQFLDVGKAALLDDFDDSPDITKSGLYQKVY
AAEYGQFGGQPIGAIVANYTFGPGAQDVKLLQYVASTSAMAHTPFIAAAGPAFFGIDSFGKLPNVKDLASLFEGPQFAKW
NAFRESEDARYVGLTLPRFLLRLPYGANTTPVKRFNYDERVDGGDADFLWGNAAFAFATRLTASFADYRWCANVIGPKGG
GTVADLPLYAYEAMGEIQNKIPTDVLISERREFELAEQGFIALTMRKHSDNAAFFSANSTQKPKFFGISKEGKDAELNYR
LGTQLPYIFVVNRLAHYIKVIQRENIGTWKERGDLEQELNQWIRQYVADMDNPTEGVRSRRPLRQAEIFVSDVEGEPGWY
RVDMKVRPHFKYMGASFTLSLVGKLEKR*

  Nucleotide sequence: 1527 bp    

>T6CP001070 NC_007650:c1020794-1019268 [Burkholderia thailandensis E264] [TssC]
ATGAACCGCGACACGGAACGGACGACCATGGAAGGCGAACACCTGTACTCCCCGAAAAACGACGACGCGCCGCCCGCGCC
GGCGCCCGATTCACCCGCATCGCTGCTCGACGAGCTGATCGAGGCCGCGCGCGTGAAGCGCGACGAGGAAGCGTATCCGA
TCACGCGCCACGGCATCGAGGCATTCGTCGCGCATCTGGCGCGGCCGAAGCGCCCGATCGAGACCGTGAGCCAGGCGACG
ATCGACGACATGATCGCCGAGATCGACCGCAAGCTGTGCCGGCAGGTCGACGCGATCCTCCACCACCCGGATTTCCAGCA
GCTCGAATCGACGTGGCGCTCGCTGAAGTTCCTCGTCGAGCGCACGGATTTCCGCGAGAACATCAAGATCCAGTTTCTCG
ACGTCGGCAAGGCGGCGCTCCTCGACGATTTCGACGATTCGCCCGACATCACGAAATCCGGCCTGTACCAGAAGGTCTAT
GCGGCCGAGTACGGCCAGTTCGGCGGCCAGCCGATCGGCGCGATCGTCGCGAACTATACGTTCGGGCCCGGCGCGCAGGA
TGTGAAGCTGCTGCAATACGTCGCGAGCACGTCGGCGATGGCGCACACGCCGTTCATCGCGGCGGCGGGCCCCGCGTTCT
TCGGCATCGATTCGTTCGGCAAGCTGCCGAACGTGAAGGATCTGGCTTCGCTGTTCGAGGGGCCGCAGTTCGCGAAGTGG
AACGCGTTTCGCGAAAGCGAGGACGCGCGCTACGTCGGCCTCACGCTGCCGCGCTTCCTGCTGCGGCTGCCGTACGGCGC
GAACACGACGCCCGTCAAGCGCTTCAATTACGACGAGCGCGTCGACGGCGGCGACGCGGATTTTCTCTGGGGCAACGCCG
CGTTCGCGTTCGCGACGCGGCTCACCGCGAGCTTCGCCGACTATCGCTGGTGCGCGAACGTGATCGGCCCGAAGGGCGGC
GGCACGGTCGCCGATCTGCCGCTCTATGCGTACGAGGCGATGGGCGAGATCCAGAACAAGATCCCGACCGACGTGCTGAT
TTCCGAGCGCCGCGAATTCGAGCTCGCCGAGCAGGGGTTCATCGCGCTCACGATGCGCAAGCACAGCGACAACGCCGCGT
TCTTCTCCGCCAATTCGACGCAAAAGCCGAAGTTCTTCGGCATCAGCAAGGAAGGCAAGGATGCCGAACTGAATTACCGG
CTCGGCACCCAGCTGCCGTACATCTTCGTCGTCAACCGGCTCGCGCATTACATCAAGGTGATCCAGCGCGAGAACATCGG
CACGTGGAAGGAGCGCGGCGATCTCGAGCAGGAACTCAACCAGTGGATCCGCCAGTACGTCGCGGACATGGACAACCCGA
CCGAGGGCGTGCGCAGCCGCCGGCCGCTGCGCCAGGCGGAGATCTTCGTGTCGGACGTCGAGGGCGAGCCGGGCTGGTAC
CGCGTCGACATGAAGGTTCGGCCGCATTTCAAGTACATGGGCGCGTCGTTCACGCTGTCGCTCGTCGGCAAGCTCGAGAA
GCGCTGA