Detailed information of component protein


Summary

Component ID T6CP001063
Component protein type TssL
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (226 a.a.); Nucleotide sequence (678 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04445
Coordinate (Strand) 1001572..1002249 (-)
NCBI ID WP_025370195.1
RefSeq NC_007650
Uniprot ID UPI00030BC63F
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.1e-41 3..210

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-190 MKLSDSMMPLVAYARQLAQSPRGDAAEVALRLDILIERARDQAACAGAPEADVDDALFAVCAWIDEALLNSGWNDAERWTLRLLQKRYFDTSHAGVVFFERLDALDGARADVLDVYLLCLQLGFRGRYGYDGGSGPLDAIRQRALDALSAHAPPCAAHALPFPAAYPHAETQPGAHAIAAKARARRRIAL
Transmembrane helix 191-213 AGRNFGLPLAILLSLYLIYHAVI
Outside 214-225 VRMVDSVFPRLQ



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9530 - -



  Protein sequence: 226 a.a.    

>T6CP001063 NC_007650:c1002249-1001572 [Burkholderia thailandensis E264] [TssL]
MKLSDSMMPLVAYARQLAQSPRGDAAEVALRLDILIERARDQAACAGAPEADVDDALFAVCAWIDEALLNSGWNDAERWT
LRLLQKRYFDTSHAGVVFFERLDALDGARADVLDVYLLCLQLGFRGRYGYDGGSGPLDAIRQRALDALSAHAPPCAAHAL
PFPAAYPHAETQPGAHAIAAKARARRRIALAGRNFGLPLAILLSLYLIYHAVIVRMVDSVFPRLQ*

  Nucleotide sequence: 678 bp    

>T6CP001063 NC_007650:c1002249-1001572 [Burkholderia thailandensis E264] [TssL]
ATGAAGCTGTCCGATTCGATGATGCCGCTCGTCGCCTATGCGCGGCAGCTCGCGCAATCGCCGCGCGGCGACGCCGCCGA
AGTCGCGCTGCGGCTCGACATCCTGATCGAGCGCGCGCGCGACCAGGCGGCGTGCGCCGGCGCGCCCGAAGCCGACGTCG
ACGACGCGCTGTTCGCGGTGTGCGCGTGGATCGACGAGGCGCTGCTGAACAGCGGCTGGAACGACGCCGAGCGCTGGACG
CTGAGGCTGCTGCAAAAGCGTTACTTCGATACATCGCACGCGGGCGTCGTGTTCTTCGAGCGCCTCGACGCGCTCGACGG
CGCGCGCGCGGACGTGCTCGACGTCTATCTGCTGTGCCTGCAGCTCGGCTTTCGCGGCCGCTACGGCTACGACGGCGGCA
GCGGGCCGCTCGACGCGATCCGGCAGCGCGCGCTCGACGCGCTGTCGGCGCACGCGCCGCCGTGCGCCGCGCACGCGCTG
CCGTTTCCCGCCGCGTATCCGCACGCCGAGACCCAGCCCGGCGCGCACGCCATCGCGGCGAAGGCGCGCGCGCGCCGCCG
CATCGCGCTCGCCGGACGGAACTTCGGGCTGCCGCTCGCGATCCTGCTGTCGCTTTATCTGATCTATCACGCCGTCATCG
TGCGGATGGTCGACTCGGTATTCCCGAGGCTGCAATGA