Detailed information of component protein


Summary

Component ID T6CP001067
Component protein type TssH
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (959 a.a.); Nucleotide sequence (2877 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04485
Coordinate (Strand) 1012489..1015365 (-)
NCBI ID WP_009896189.1
RefSeq NC_007650
Uniprot ID Q2T6Y8_BURTA
KEGG ID bte:BTH_II0864
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 7.3e-40 640..807
AAA_lid_9 PF17871.3 2.7e-33 367..458
ClpB_D2-small PF10431.11 2.8e-11 812..881
AAA PF00004.31 2.1e-07 229..338

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-958 MLLVDLKPLIERLNPYCRTALENAVGACVARRHDDVAVEHLLARLCDEPSADVALLLRASGADAARLRRQADAALDARPAGGGGRPAFAPSLLALLQDAWLIASLELGETQIRSAAVLAAAVARAARQPSRGGDDMLQSLRRDALVARFAHACAQSIESRGPGARDAAPGDANDTPGAHDASSAIARYCEDFTAKARAGRIDPVFGRDAQIRQIVDILARRRKNNPVCVGEPGVGKTAVVEGLALRIAEGDVPAALRHATLLGLDLGLLQAGASVKGEFEQRLKRVIAEIHASQAPVVLFVDEAHTLIGAGGPAGASDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQPVKLDSPDVATSVMILRGLKERYQDAHGVTIRDDALVAAAELSERYITGRQLPDKAIDLLDTACARVKVRQQTKPAALEDAERAIQALERERRALRRDLAERCAPDTPRVADIDRELAALSARADALSEAWAAQRDAAQALVEARRACRAIADAAEAAETASASGVAGVAGVAAAADVAPGAEASADARERAARALADATHRFEHARRDAPLVRIDVDPEAIADVVADWTGIAAGKLRRDRANVMLRLADTLKGRIRGQDHAIEQISEAVKAGAAGVHDPRRPLGVFLLAGPSGTGKTETALAVADALFGDERSIVVVNMSEFQERHDVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKAHPDVLNLFYQVFDKGSLCDGEGKEVDFANTVIFLTSNLGADVIADLAARGGRPDPDAMRAAIRPALSRHFKPALLARMTEIPYATLAPDTLADIARRKLERIAARVAAQHATRVVYDEAVVAHVAARCTEVESGARSVDFILQRHVLPALAQHVLVCAGDATPMAAIRIAIDANGRFVVSTDEPACSGGRNAADALDGPDASDTPNAPNAPNAPNAPNAPNAPNAPNAPNAR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9901 - -



  Protein sequence: 959 a.a.    

>T6CP001067 NC_007650:c1015365-1012489 [Burkholderia thailandensis E264] [TssH]
MLLVDLKPLIERLNPYCRTALENAVGACVARRHDDVAVEHLLARLCDEPSADVALLLRASGADAARLRRQADAALDARPA
GGGGRPAFAPSLLALLQDAWLIASLELGETQIRSAAVLAAAVARAARQPSRGGDDMLQSLRRDALVARFAHACAQSIESR
GPGARDAAPGDANDTPGAHDASSAIARYCEDFTAKARAGRIDPVFGRDAQIRQIVDILARRRKNNPVCVGEPGVGKTAVV
EGLALRIAEGDVPAALRHATLLGLDLGLLQAGASVKGEFEQRLKRVIAEIHASQAPVVLFVDEAHTLIGAGGPAGASDAA
NLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQPVKLDSPDVATSVMILRGLKERYQDAHGVTIRDDALVAAAEL
SERYITGRQLPDKAIDLLDTACARVKVRQQTKPAALEDAERAIQALERERRALRRDLAERCAPDTPRVADIDRELAALSA
RADALSEAWAAQRDAAQALVEARRACRAIADAAEAAETASASGVAGVAGVAAAADVAPGAEASADARERAARALADATHR
FEHARRDAPLVRIDVDPEAIADVVADWTGIAAGKLRRDRANVMLRLADTLKGRIRGQDHAIEQISEAVKAGAAGVHDPRR
PLGVFLLAGPSGTGKTETALAVADALFGDERSIVVVNMSEFQERHDVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLL
DEVEKAHPDVLNLFYQVFDKGSLCDGEGKEVDFANTVIFLTSNLGADVIADLAARGGRPDPDAMRAAIRPALSRHFKPAL
LARMTEIPYATLAPDTLADIARRKLERIAARVAAQHATRVVYDEAVVAHVAARCTEVESGARSVDFILQRHVLPALAQHV
LVCAGDATPMAAIRIAIDANGRFVVSTDEPACSGGRNAADALDGPDASDTPNAPNAPNAPNAPNAPNAPNAPNAPNAR*

  Nucleotide sequence: 2877 bp    

>T6CP001067 NC_007650:c1015365-1012489 [Burkholderia thailandensis E264] [TssH]
ATGCTGCTGGTTGACCTGAAGCCTTTGATCGAACGGCTCAACCCCTATTGCCGGACGGCGCTCGAAAACGCCGTCGGCGC
CTGCGTCGCGCGCCGGCACGACGACGTCGCCGTCGAGCATCTGCTCGCGCGGCTGTGCGACGAGCCGTCGGCCGATGTCG
CGCTGCTGCTGCGCGCGAGCGGCGCCGATGCCGCGCGCCTGCGCCGGCAGGCCGACGCGGCGCTCGACGCGCGGCCGGCG
GGCGGCGGCGGCCGTCCCGCGTTCGCGCCGTCGCTGCTCGCGCTGCTGCAGGACGCGTGGCTGATCGCGTCGCTCGAGCT
CGGCGAGACGCAGATCCGCTCGGCGGCCGTGCTGGCCGCCGCGGTCGCGCGCGCCGCGCGGCAGCCGTCGCGCGGCGGCG
ACGACATGCTGCAGTCGCTGCGCAGGGACGCACTCGTCGCGCGCTTTGCGCACGCCTGCGCGCAATCAATCGAATCGCGC
GGGCCGGGCGCGCGCGACGCCGCGCCGGGCGACGCGAACGATACGCCCGGCGCACACGACGCCTCGAGCGCGATCGCCCG
CTATTGCGAGGACTTCACCGCGAAAGCGCGCGCGGGCCGGATCGATCCGGTGTTCGGCCGCGATGCGCAGATCCGCCAGA
TCGTCGACATCCTCGCGCGGCGGCGCAAGAACAATCCGGTGTGCGTGGGCGAGCCGGGCGTCGGCAAGACGGCCGTCGTC
GAGGGGCTCGCGCTGCGGATCGCCGAAGGCGACGTGCCGGCTGCGCTGCGCCACGCGACGCTGCTCGGCCTCGATCTCGG
GCTGCTGCAGGCGGGCGCGAGCGTCAAGGGCGAATTCGAGCAGCGGCTCAAGCGCGTGATCGCCGAAATCCATGCGTCGC
AAGCGCCCGTCGTGCTGTTCGTCGACGAGGCGCACACGCTGATCGGCGCGGGCGGCCCGGCGGGCGCGTCCGACGCGGCG
AACCTGCTGAAGCCCGCGCTCGCGCGCGGCGAGCTGCGCACGATCGCGGCCACCACGTGGAGCGAATACAAGAAGTACTT
CGAGAAGGACGCGGCGCTCGCGCGGCGCTTTCAGCCGGTGAAGCTCGACAGCCCGGACGTCGCGACGTCGGTAATGATCC
TGCGCGGCCTGAAGGAGCGCTATCAGGACGCGCACGGCGTGACGATCCGCGACGACGCGCTCGTCGCCGCGGCCGAGCTG
TCGGAGCGCTACATCACCGGCCGCCAGTTGCCGGACAAGGCGATCGACCTGCTCGACACGGCCTGCGCGCGCGTGAAGGT
GCGCCAGCAGACGAAGCCCGCCGCGCTGGAGGACGCCGAGCGCGCGATCCAGGCGCTCGAGCGCGAGCGGCGCGCGCTGC
GGCGGGATCTGGCCGAGCGATGCGCGCCGGACACGCCGCGCGTCGCCGACATCGATCGCGAGCTCGCCGCGCTGTCCGCG
CGCGCCGACGCGCTGAGCGAAGCGTGGGCGGCGCAGCGCGACGCGGCGCAAGCGCTCGTCGAAGCGCGCCGCGCGTGCCG
GGCGATCGCGGATGCCGCCGAGGCGGCCGAGACGGCCAGCGCGAGCGGTGTTGCCGGTGTGGCCGGTGTGGCCGCCGCAG
CGGATGTCGCCCCTGGTGCCGAGGCCAGCGCCGACGCACGCGAGCGCGCCGCGCGCGCGCTCGCCGACGCGACCCATCGT
TTCGAGCATGCGCGGCGCGACGCGCCGCTCGTGCGAATCGATGTCGATCCGGAAGCGATCGCCGACGTCGTGGCCGACTG
GACCGGCATCGCGGCCGGCAAGCTGCGCCGCGATCGCGCGAACGTGATGCTGCGGCTCGCCGATACGCTCAAAGGCCGCA
TCCGCGGCCAGGATCACGCGATCGAGCAGATCTCGGAAGCCGTGAAGGCGGGCGCGGCCGGCGTCCACGATCCGCGCCGC
CCGCTCGGCGTGTTCCTGCTCGCGGGGCCGTCGGGCACCGGCAAGACCGAGACCGCGCTCGCCGTCGCCGACGCGCTGTT
CGGCGACGAGCGCTCGATCGTCGTCGTCAACATGAGCGAATTCCAGGAGCGGCACGACGTGAGCCGGCTGATCGGCTCGC
CGCCCGGCTACGTCGGCTACGGCGAAGGCGGGATGCTGACCGAGGCGGTGCGCCAGCGGCCCTATTCGGTCGTGCTGCTC
GACGAAGTCGAGAAAGCCCACCCGGACGTGCTGAACCTGTTCTATCAGGTGTTCGACAAGGGCTCGCTGTGCGACGGCGA
AGGCAAGGAAGTCGACTTCGCGAACACGGTGATCTTCCTCACGTCGAATCTCGGCGCGGACGTCATCGCCGATCTCGCGG
CGCGCGGCGGCCGCCCCGATCCGGACGCGATGCGCGCGGCGATCCGGCCGGCGCTGTCGCGCCATTTCAAGCCGGCGCTG
CTCGCGCGAATGACCGAGATTCCGTATGCGACGCTCGCGCCCGACACGCTCGCCGACATCGCGCGGCGCAAGCTCGAGCG
CATCGCGGCGCGCGTCGCCGCGCAGCACGCGACGCGCGTCGTCTACGACGAAGCCGTCGTCGCGCATGTCGCCGCGCGCT
GCACCGAGGTCGAATCGGGCGCGCGCAGCGTCGACTTCATCCTGCAACGGCACGTGCTGCCCGCGCTCGCGCAGCACGTG
CTCGTGTGCGCGGGCGATGCGACGCCGATGGCCGCGATCCGGATCGCAATCGACGCCAACGGCCGCTTCGTCGTCTCGAC
CGACGAGCCGGCCTGCTCCGGCGGGCGGAACGCGGCCGATGCGCTCGACGGGCCCGACGCATCCGATACGCCCAACGCGC
CCAACGCGCCCAACGCGCCCAACGCGCCCAACGCGCCCAACGCGCCCAACGCGCCCAACGCGCCCAACGCGCGCTGA