Detailed information of component protein


Summary

Component ID T6CP008467
Component protein type TssA
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (599 a.a.); Nucleotide sequence (1797 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04530
Coordinate (Strand) 1024090..1025886 (+)
NCBI ID WP_009896202.1
RefSeq NC_007650
Uniprot ID Q2T6X9_BURTA
KEGG ID bte:BTH_II0873
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 1.9e-71 286..559
ImpA_N PF06812.14 1.9e-25 31..143

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-598 MGMNERRQPGGAASGALLPEDFDALGALGRADIDPAAPAGADVRADARFDALHAELAKLASPGASGHVDWRAAMSLAAGLLRDRGKDLLVGCYLAGALLQIGGAAGLRCGLEVVGDLVERHWAAMSPPVSRMRARRGALQWLLDRVDATRDAGAAACGAACSVELVEQLRAAARRIDALLAERDDEAPTMRAVTAFAARLPVESGESGEPGEPGEPGEPGEPGEPGESNSTHAHGSVGAPAERAALSFAEHASIEPAGRAAPRANADAARHPASLDDAAGRERALADALAQLHRIATAFAQADWADTRGFRLRRIACWSSVHAMPDTEADSGRTRIAAPNAQVVDVAKGIDAQGDPAAAVRFAEEHAQAFPLWLDLQRIAARALARAGGDCTGAQREVETAVRALLMRLPGLDALKFADGTPFADAATRAWLAELCTPIGAANAALTSPPPPSPPAPSLPMTGESDRARGDARDANADDAHARARALAASGRLDLALGAIQQAIDRAPSAERRLRARIRLCEFARDHWEHEIPDAFARGVIEPIRRHDLLAWEPELALDGLSAAYALLIRRDGDSAHARAVLNEIAGVDAARAMRLST



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.7187 - -



  Protein sequence: 599 a.a.    

>T6CP008467 NC_007650:1024090-1025886 [Burkholderia thailandensis E264] [TssA]
MGMNERRQPGGAASGALLPEDFDALGALGRADIDPAAPAGADVRADARFDALHAELAKLASPGASGHVDWRAAMSLAAGL
LRDRGKDLLVGCYLAGALLQIGGAAGLRCGLEVVGDLVERHWAAMSPPVSRMRARRGALQWLLDRVDATRDAGAAACGAA
CSVELVEQLRAAARRIDALLAERDDEAPTMRAVTAFAARLPVESGESGEPGEPGEPGEPGEPGEPGESNSTHAHGSVGAP
AERAALSFAEHASIEPAGRAAPRANADAARHPASLDDAAGRERALADALAQLHRIATAFAQADWADTRGFRLRRIACWSS
VHAMPDTEADSGRTRIAAPNAQVVDVAKGIDAQGDPAAAVRFAEEHAQAFPLWLDLQRIAARALARAGGDCTGAQREVET
AVRALLMRLPGLDALKFADGTPFADAATRAWLAELCTPIGAANAALTSPPPPSPPAPSLPMTGESDRARGDARDANADDA
HARARALAASGRLDLALGAIQQAIDRAPSAERRLRARIRLCEFARDHWEHEIPDAFARGVIEPIRRHDLLAWEPELALDG
LSAAYALLIRRDGDSAHARAVLNEIAGVDAARAMRLST*

  Nucleotide sequence: 1797 bp    

>T6CP008467 NC_007650:1024090-1025886 [Burkholderia thailandensis E264] [TssA]
ATGGGAATGAACGAACGGCGTCAGCCCGGCGGCGCGGCCTCGGGTGCGCTTCTTCCCGAGGATTTCGACGCGCTCGGGGC
GCTGGGGCGCGCGGACATCGATCCGGCCGCGCCCGCGGGCGCCGACGTGCGCGCCGATGCGCGGTTCGATGCGCTGCACG
CGGAACTCGCGAAGCTCGCGTCGCCGGGCGCGAGCGGGCACGTCGACTGGCGCGCGGCGATGAGCCTCGCCGCCGGGTTG
CTCCGCGATCGCGGCAAGGATCTGCTCGTCGGCTGCTATCTGGCGGGCGCGCTGCTGCAGATCGGCGGCGCGGCGGGATT
GCGCTGCGGGCTCGAAGTCGTCGGCGATCTCGTCGAACGTCATTGGGCGGCGATGTCGCCGCCGGTGTCGCGGATGCGGG
CGCGGCGCGGCGCGCTGCAATGGCTGCTCGATCGCGTCGACGCGACGCGCGACGCCGGCGCCGCCGCGTGCGGTGCCGCC
TGTTCGGTCGAACTGGTCGAGCAATTGCGCGCGGCCGCGCGGCGCATCGACGCGCTGCTGGCGGAGCGCGACGACGAGGC
GCCGACGATGCGCGCGGTGACCGCGTTCGCGGCCCGGCTGCCGGTCGAGTCGGGCGAGTCGGGCGAGCCGGGCGAGCCGG
GCGAGCCGGGTGAGCCGGGTGAGCCGGGTGAGCCGGGTGAGTCGAATTCGACGCACGCCCACGGCTCGGTGGGCGCGCCT
GCCGAGCGCGCCGCGCTTTCCTTCGCCGAGCATGCATCGATCGAGCCGGCCGGCCGCGCGGCGCCGCGCGCGAACGCCGA
CGCCGCCCGGCACCCGGCGTCGCTCGACGACGCGGCCGGCCGCGAGCGCGCGCTCGCCGATGCGCTCGCGCAACTGCATC
GCATCGCGACGGCGTTCGCGCAAGCGGACTGGGCCGACACGCGCGGCTTCCGGCTGCGCCGCATCGCGTGCTGGTCGAGC
GTGCACGCGATGCCGGATACCGAAGCGGACAGCGGACGGACGCGGATCGCCGCGCCGAATGCGCAGGTTGTAGACGTCGC
AAAGGGTATCGATGCGCAGGGCGACCCCGCGGCTGCCGTCCGCTTCGCGGAGGAGCACGCGCAGGCGTTCCCGCTCTGGC
TGGATTTGCAGCGGATCGCCGCGCGCGCGCTCGCACGCGCGGGCGGCGATTGCACCGGGGCGCAACGCGAAGTGGAGACG
GCGGTGCGTGCGCTGCTCATGCGCTTGCCGGGCCTCGACGCACTGAAATTCGCGGACGGCACGCCGTTCGCCGACGCCGC
GACGCGCGCGTGGCTCGCGGAGCTATGCACGCCGATCGGTGCGGCAAATGCCGCGTTGACATCGCCGCCGCCGCCTTCGC
CGCCGGCGCCGTCCTTGCCGATGACGGGCGAGTCGGATCGTGCGCGGGGCGATGCGCGCGACGCGAATGCGGACGACGCG
CACGCCCGTGCGCGCGCGCTTGCCGCAAGCGGTCGGCTCGATCTCGCGCTGGGTGCGATTCAGCAGGCGATCGATCGCGC
GCCGAGCGCCGAACGCCGGTTGAGGGCGCGCATCCGGCTGTGCGAGTTCGCTCGCGATCACTGGGAACACGAGATTCCCG
ACGCGTTCGCGCGCGGCGTGATCGAGCCGATCCGGCGGCACGACTTGCTCGCATGGGAGCCCGAACTGGCGCTTGACGGC
TTGTCGGCCGCCTATGCGCTGCTGATTCGGCGTGATGGTGATTCGGCGCACGCACGGGCTGTGCTTAACGAGATCGCGGG
CGTCGACGCGGCGCGGGCGATGCGTTTGTCGACGTGA