Detailed information of component protein


Summary

Component ID T6CP001065
Component protein type TssJ
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (269 a.a.); Nucleotide sequence (807 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[6] Lennings J, Makhlouf M, Olejnik P, et al. Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis. Int J Med Microbiol. 2019 Sep;309(6):151335. doi: 10.1016/j.ijmm.2019.151335. Epub 2019 Jul 18. PMID: 31378704
[7] Lennings J, Mayer C, Makhlouf M, et al. Polar localization of the ATPase ClpV-5 occurs independent of type VI secretion system apparatus proteins in Burkholderia thailandensis. BMC Res Notes. 2019 Feb 28;12(1):109. doi: 10.1186/s13104-019-4141-3. PMID: 30819219
[8] Lennings J, West TE, Schwarz S. The Burkholderia Type VI Secretion System 5: Composition, Regulation and Role in Virulence. Front Microbiol. 2019 Jan 10;9:3339. doi: 10.3389/fmicb.2018.03339. eCollection 2018. PMID: 30687298
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS04455
Coordinate (Strand) 1003676..1004482 (-)
NCBI ID WP_011401053.1
RefSeq NC_007650
Uniprot ID Q2T6Z4_BURTA, A0A7X3DTG9_BURTH
KEGG ID bte:BTH_II0858
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 9.3e-17 75..183
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-268 MSDRRIFMPAAAQARRPTQPAQACRSSRQLRPRRACAARACAATLAALAALAGCAAGVTEEQARADVRWDYAPDALRIDIDASPRLNEYLNAPHTLLLAVFQSADARTFRQLADDPDRLRMTLAAGGPATDFIQTTRYVVEPGARVALSIDRAQQARYVGIVAGYYDADSPRAARLFDVPLRIDKRGWFSSTYRAAPRTLGLKLRLGAQSITDAREAPLNLPPPGARAWTTLDGGAKTLTLPAGDANGSENGGGGDENDASAHAPRKR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
TAT(Tat/SPI) 0.4103 pos: 65-66. ARA-DV. MSDRRIFMPAAAQARRPTQPAQACRSSRQLRPRRACAARACAATLAALAALAGCAAGVTEEQARA


  Protein sequence: 269 a.a.    

>T6CP001065 NC_007650:c1004482-1003676 [Burkholderia thailandensis E264] [TssJ]
MSDRRIFMPAAAQARRPTQPAQACRSSRQLRPRRACAARACAATLAALAALAGCAAGVTEEQARADVRWDYAPDALRIDI
DASPRLNEYLNAPHTLLLAVFQSADARTFRQLADDPDRLRMTLAAGGPATDFIQTTRYVVEPGARVALSIDRAQQARYVG
IVAGYYDADSPRAARLFDVPLRIDKRGWFSSTYRAAPRTLGLKLRLGAQSITDAREAPLNLPPPGARAWTTLDGGAKTLT
LPAGDANGSENGGGGDENDASAHAPRKR*

  Nucleotide sequence: 807 bp    

>T6CP001065 NC_007650:c1004482-1003676 [Burkholderia thailandensis E264] [TssJ]
ATGAGCGATCGACGGATTTTCATGCCGGCGGCAGCGCAAGCGCGCCGGCCGACGCAACCGGCGCAAGCTTGCCGATCATC
ACGGCAGTTGCGGCCACGCCGCGCATGCGCGGCGCGCGCGTGCGCGGCCACGCTCGCCGCGCTGGCCGCGCTGGCCGGCT
GCGCGGCCGGCGTCACCGAGGAGCAGGCGCGCGCCGACGTGCGATGGGACTACGCGCCCGACGCGCTGCGCATCGACATC
GACGCGTCGCCACGGCTCAACGAATACCTGAACGCGCCGCACACGTTGCTGCTCGCGGTGTTCCAAAGCGCGGACGCGCG
GACGTTCCGCCAGCTCGCCGACGATCCGGACCGGCTGCGCATGACGCTCGCGGCGGGCGGCCCCGCGACGGACTTCATCC
AGACGACGCGCTACGTGGTCGAGCCGGGCGCGCGCGTCGCGCTGTCGATCGATCGTGCGCAGCAGGCGCGCTACGTCGGC
ATCGTCGCCGGCTACTACGACGCCGACAGCCCGCGCGCCGCGCGCCTCTTCGACGTGCCGCTGCGAATCGACAAGCGCGG
CTGGTTCTCGTCCACCTACCGCGCCGCGCCGCGAACGCTCGGGCTGAAGCTGCGGCTCGGCGCGCAGAGCATCACCGACG
CGCGCGAAGCCCCGCTGAACCTGCCGCCGCCGGGCGCGCGCGCGTGGACGACGCTCGACGGCGGCGCGAAAACGCTGACG
CTGCCGGCCGGCGACGCGAACGGAAGCGAAAACGGCGGCGGCGGCGACGAAAACGACGCCTCCGCCCACGCGCCGCGCAA
GCGATGA