Detailed information of component protein


Summary

Component ID T6CP001071
Component protein type TssB
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (165 a.a.); Nucleotide sequence (495 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04515
Coordinate (Strand) 1020791..1021285 (-)
NCBI ID WP_009896198.1
RefSeq NC_007650
Uniprot ID A0A1B4K3G5_BURTH, Q2T6Y2_BURTA
KEGG ID btha:DR62_3409, bte:BTH_II0870
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VipA PF05591.14 2.5e-52 6..159

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-164 MTTNGSVAPKERVNIVYRPATGDAKAEVELPLKVLVLGEFSTADDKPPIEELAPVNVDKDDFNDVMKAQRLTLALSVPNLLDDQAGEDDRLSVALGFESIADFSPDAIVETVPELKQLVALRDALKALKGPLGNIPGFRKRIQEIVADKGARKQLLDELGLDEQ



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9777 - -



  Protein sequence: 165 a.a.    

>T6CP001071 NC_007650:c1021285-1020791 [Burkholderia thailandensis E264] [TssB]
MTTNGSVAPKERVNIVYRPATGDAKAEVELPLKVLVLGEFSTADDKPPIEELAPVNVDKDDFNDVMKAQRLTLALSVPNL
LDDQAGEDDRLSVALGFESIADFSPDAIVETVPELKQLVALRDALKALKGPLGNIPGFRKRIQEIVADKGARKQLLDELG
LDEQ*

  Nucleotide sequence: 495 bp    

>T6CP001071 NC_007650:c1021285-1020791 [Burkholderia thailandensis E264] [TssB]
ATGACCACGAACGGATCCGTCGCGCCGAAAGAGCGGGTCAACATCGTCTATCGCCCGGCCACGGGCGACGCGAAAGCGGA
AGTCGAGCTGCCGCTGAAGGTGCTCGTGCTCGGCGAATTCTCGACGGCCGACGACAAGCCGCCGATCGAGGAGCTCGCCC
CCGTGAACGTCGACAAGGACGATTTCAACGACGTGATGAAGGCGCAGCGCCTGACGCTCGCGCTCAGCGTGCCGAACCTG
CTCGACGATCAGGCGGGCGAGGACGATCGCCTGAGCGTCGCGCTCGGATTCGAATCGATCGCCGACTTCTCGCCCGACGC
GATCGTCGAGACGGTGCCCGAGCTGAAGCAGCTCGTCGCGCTGCGCGACGCGCTGAAGGCGCTCAAGGGCCCGCTCGGCA
ACATCCCGGGCTTTCGCAAGCGCATCCAGGAAATCGTCGCGGACAAGGGCGCGCGCAAGCAACTGCTCGACGAACTGGGC
CTCGACGAACAATGA