Detailed information of component protein


Summary

Component ID T6CP001066
Component protein type TssI
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (1013 a.a.); Nucleotide sequence (3039 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[6] Lennings J, Makhlouf M, Olejnik P, et al. Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis. Int J Med Microbiol. 2019 Sep;309(6):151335. doi: 10.1016/j.ijmm.2019.151335. Epub 2019 Jul 18. PMID: 31378704
[7] Lennings J, Mayer C, Makhlouf M, et al. Polar localization of the ATPase ClpV-5 occurs independent of type VI secretion system apparatus proteins in Burkholderia thailandensis. BMC Res Notes. 2019 Feb 28;12(1):109. doi: 10.1186/s13104-019-4141-3. PMID: 30819219
[8] Lennings J, West TE, Schwarz S. The Burkholderia Type VI Secretion System 5: Composition, Regulation and Role in Virulence. Front Microbiol. 2019 Jan 10;9:3339. doi: 10.3389/fmicb.2018.03339. eCollection 2018. PMID: 30687298
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS04480
Coordinate (Strand) 1009425..1012463 (-)
NCBI ID WP_009896187.1
RefSeq NC_007650
Uniprot ID Q2T6Y9_BURTA, A0A7X3J4M1_BURTH
KEGG ID bte:BTH_II0863
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.2e-70 58..361
Phage_base_V PF04717.14 1e-06 426..492
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1012 MSSSHRHYADTALADVAALTDAASRADAAPLANARRFTFASTAYDAATFDVVDIDGRDAISQPYRFEITLVSRSVRIDFAKMLSCEATLAILPPFGEAGTTRYAGVLAEFEQKERFRDFTVYRAALVPRLWRLSLYKASDVYLNEQTIPDIVKRVLRAASFGKRNFRMRHRGVYRKRSFVCQYDESHLDFVSRWMEKEGLYYYFEHDGRREKLEIVDDRRDQPGPADDLALRYLPATCLDAGIESDRVQAFACRATPLPREVVLRDFNHRKAELSLEVREHVAHDGVGERVSSDEHFHTKDEGRRYAKLRAEALVCEGRRFAGESTAAGLRAGRFFALSGHYRKDFDGRYLVTAVTHRGSQAHLLFPDLDAPFGATPGEPVYRAEFEAIAANLQYRPPRTTPKPRAAGVVSAIVDGEGSGKRAELDEHGQYKVRFPFAHTAHPTNKASARIRMATPYAGDDRGMHLPLLKRTEVKIAFDGGDPDRPVIVGAVPNSSHRSVVTRSNPDAHRILTEHNQLYMKDGSGAATWLHAPNNHIGIGAVGPGDGLALLTSGNKFDFSLGNAYSFSGGLKCSVSMGGNTDIYVGVRNSLDVSANFLTTLQGNLRWMLPGSRSFEINDSASTLLQTLHKQSATGAIRLSAGQDASALLQKQLDKLKGTVRKFMIVSGLANAGAAATAAGLIKGGGALADLPWAGFGVSAAQFAGATGFSTALMATSRTLLSKIAKLQEALPLVADLSLDKQGIALAAKNLTHATRMSLTVDGVSWSTHAKGPGAAGAAMSVGKGRWGVEAAEHAHVHANDTLLFAVPADPTSKFDLKELIGLRRDLDECVKGIADLEADISENEVLSTDQNTFGVGALVPTPPSPANAVAAVAIKAKEAKLVELNAKRKLVATKIDNLQQKLAKHAKNLSAARMSASDAEVGFKGNRLVATAEGVTLAHAQGKAKLDVREAKIGVEAGKSSLELDESKLAAGCGGASLKLGSDGAIDVRATNVKLNGSASLKLDGQLIQLG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8709 - -


  Protein sequence: 1013 a.a.    

>T6CP001066 NC_007650:c1012463-1009425 [Burkholderia thailandensis E264] [TssI]
MSSSHRHYADTALADVAALTDAASRADAAPLANARRFTFASTAYDAATFDVVDIDGRDAISQPYRFEITLVSRSVRIDFA
KMLSCEATLAILPPFGEAGTTRYAGVLAEFEQKERFRDFTVYRAALVPRLWRLSLYKASDVYLNEQTIPDIVKRVLRAAS
FGKRNFRMRHRGVYRKRSFVCQYDESHLDFVSRWMEKEGLYYYFEHDGRREKLEIVDDRRDQPGPADDLALRYLPATCLD
AGIESDRVQAFACRATPLPREVVLRDFNHRKAELSLEVREHVAHDGVGERVSSDEHFHTKDEGRRYAKLRAEALVCEGRR
FAGESTAAGLRAGRFFALSGHYRKDFDGRYLVTAVTHRGSQAHLLFPDLDAPFGATPGEPVYRAEFEAIAANLQYRPPRT
TPKPRAAGVVSAIVDGEGSGKRAELDEHGQYKVRFPFAHTAHPTNKASARIRMATPYAGDDRGMHLPLLKRTEVKIAFDG
GDPDRPVIVGAVPNSSHRSVVTRSNPDAHRILTEHNQLYMKDGSGAATWLHAPNNHIGIGAVGPGDGLALLTSGNKFDFS
LGNAYSFSGGLKCSVSMGGNTDIYVGVRNSLDVSANFLTTLQGNLRWMLPGSRSFEINDSASTLLQTLHKQSATGAIRLS
AGQDASALLQKQLDKLKGTVRKFMIVSGLANAGAAATAAGLIKGGGALADLPWAGFGVSAAQFAGATGFSTALMATSRTL
LSKIAKLQEALPLVADLSLDKQGIALAAKNLTHATRMSLTVDGVSWSTHAKGPGAAGAAMSVGKGRWGVEAAEHAHVHAN
DTLLFAVPADPTSKFDLKELIGLRRDLDECVKGIADLEADISENEVLSTDQNTFGVGALVPTPPSPANAVAAVAIKAKEA
KLVELNAKRKLVATKIDNLQQKLAKHAKNLSAARMSASDAEVGFKGNRLVATAEGVTLAHAQGKAKLDVREAKIGVEAGK
SSLELDESKLAAGCGGASLKLGSDGAIDVRATNVKLNGSASLKLDGQLIQLG*

  Nucleotide sequence: 3039 bp    

>T6CP001066 NC_007650:c1012463-1009425 [Burkholderia thailandensis E264] [TssI]
ATGTCTTCGTCCCATCGACACTACGCCGACACGGCGCTCGCGGATGTCGCGGCGCTCACGGATGCCGCGTCGCGCGCCGA
CGCCGCGCCGCTCGCGAACGCGCGACGCTTCACGTTCGCGAGCACCGCGTACGACGCTGCCACGTTCGACGTCGTCGACA
TCGACGGCCGCGACGCGATCTCGCAGCCGTACCGGTTCGAGATCACGCTCGTGAGCAGGAGTGTGCGGATCGACTTCGCG
AAGATGCTGAGTTGCGAGGCGACGCTCGCGATCCTGCCGCCGTTCGGTGAGGCCGGCACGACCCGCTATGCCGGCGTGCT
CGCCGAATTCGAGCAGAAGGAACGCTTTCGCGACTTCACCGTCTATCGCGCGGCGCTCGTGCCGCGCCTCTGGCGCCTCT
CGCTGTACAAGGCGTCGGACGTCTACCTGAACGAGCAGACGATTCCCGATATCGTCAAGCGCGTGCTGCGCGCCGCGTCG
TTCGGCAAGCGCAATTTCCGCATGCGGCACCGCGGCGTCTACCGCAAGCGCAGCTTCGTCTGCCAGTACGACGAGAGCCA
TCTCGATTTCGTGTCGCGCTGGATGGAGAAGGAAGGCCTCTACTACTACTTCGAGCATGACGGCCGACGCGAAAAGCTCG
AGATCGTTGACGACCGCCGCGACCAGCCCGGCCCCGCCGACGATCTCGCGCTGCGCTACCTACCCGCCACCTGTCTCGAC
GCGGGCATCGAATCGGACCGCGTGCAGGCGTTCGCATGCCGCGCGACGCCGCTGCCGCGCGAGGTCGTGCTGCGCGATTT
CAACCACCGCAAGGCGGAGCTGTCGCTCGAAGTCCGCGAGCACGTGGCGCACGACGGCGTCGGCGAGCGGGTATCGAGCG
ACGAGCATTTCCACACGAAGGACGAAGGGCGGCGCTACGCGAAGCTGCGCGCCGAGGCGCTCGTTTGCGAAGGCCGCCGT
TTCGCCGGCGAATCGACCGCGGCCGGGCTGCGCGCGGGCCGCTTCTTCGCGCTCTCGGGCCACTACCGCAAGGACTTCGA
CGGCCGCTATCTGGTGACGGCGGTCACGCATCGCGGCTCGCAGGCGCACCTGCTGTTTCCCGATCTCGACGCGCCGTTCG
GCGCGACGCCGGGCGAGCCCGTCTACCGCGCCGAGTTCGAGGCGATCGCCGCCAACCTCCAGTACCGGCCGCCGCGCACG
ACGCCGAAGCCGCGCGCGGCGGGCGTCGTCAGCGCGATCGTCGACGGCGAGGGCAGCGGCAAGCGCGCCGAACTCGACGA
ACACGGCCAGTACAAAGTGCGCTTTCCGTTCGCGCACACCGCGCATCCGACGAACAAGGCCTCCGCGCGCATCCGGATGG
CGACGCCCTATGCGGGCGACGACCGCGGCATGCATCTGCCGCTTCTGAAGCGCACCGAAGTGAAGATCGCATTCGACGGC
GGCGATCCGGACCGCCCCGTGATCGTCGGCGCGGTGCCCAACTCGTCGCACCGCAGCGTCGTCACGCGCAGCAACCCCGA
CGCGCACCGGATCCTCACCGAGCACAACCAGCTCTACATGAAGGACGGCAGCGGCGCGGCGACGTGGCTGCACGCGCCGA
ACAACCACATCGGCATCGGCGCGGTCGGGCCGGGCGACGGCCTCGCGCTCCTCACGTCCGGCAACAAGTTCGATTTCTCG
CTCGGCAACGCGTACAGCTTCTCGGGCGGGCTCAAGTGCTCGGTGTCGATGGGCGGCAACACCGACATCTACGTCGGCGT
GCGCAACAGCCTCGACGTCAGCGCGAACTTCCTGACGACGCTGCAGGGCAACCTGCGCTGGATGCTGCCCGGCAGCCGAA
GCTTCGAGATCAACGACAGCGCGTCCACGCTGCTGCAGACGCTGCACAAGCAGTCCGCGACGGGCGCGATCCGGCTGTCC
GCCGGGCAGGACGCGTCCGCGCTGCTGCAAAAGCAGCTCGACAAGCTCAAGGGCACGGTGCGCAAGTTCATGATCGTGTC
GGGCCTCGCGAACGCCGGAGCCGCGGCCACCGCTGCGGGGCTCATCAAGGGCGGCGGCGCGCTCGCCGATCTGCCGTGGG
CGGGCTTCGGCGTGTCCGCCGCGCAGTTCGCCGGCGCGACCGGCTTCAGCACGGCGCTGATGGCGACCTCGCGCACGCTG
CTCTCGAAAATCGCGAAGCTCCAGGAGGCGTTGCCGCTCGTCGCCGATCTATCGCTCGACAAGCAAGGCATCGCGCTCGC
GGCGAAGAACCTCACGCACGCGACGCGGATGTCGCTCACCGTCGACGGCGTCTCGTGGTCGACGCACGCGAAGGGGCCGG
GCGCGGCAGGCGCCGCGATGAGCGTCGGCAAGGGCCGCTGGGGCGTCGAAGCGGCGGAGCACGCGCATGTCCACGCGAAT
GACACGCTGCTGTTCGCCGTGCCGGCCGACCCAACGAGCAAGTTCGACCTCAAGGAGCTGATCGGGCTGCGCCGCGATCT
CGACGAATGCGTGAAGGGTATCGCCGATCTCGAAGCCGACATTTCGGAAAACGAAGTGCTTTCGACCGATCAGAACACGT
TCGGCGTCGGCGCGCTCGTGCCCACGCCGCCGTCGCCCGCCAATGCGGTCGCGGCGGTCGCGATCAAGGCGAAGGAAGCG
AAGCTCGTCGAGCTGAACGCCAAACGCAAGCTCGTCGCGACGAAGATCGACAACCTCCAGCAGAAGCTCGCGAAGCACGC
GAAGAACCTGAGCGCCGCGCGGATGAGCGCGTCGGACGCGGAAGTCGGCTTCAAGGGCAACCGGCTCGTCGCGACGGCCG
AAGGCGTCACGCTCGCGCATGCGCAGGGCAAGGCGAAGCTCGACGTGCGCGAGGCGAAGATCGGCGTCGAGGCAGGCAAA
TCGAGCCTCGAGCTCGACGAGAGCAAGCTCGCGGCCGGCTGCGGCGGCGCCTCGCTGAAGCTGGGCAGCGACGGCGCGAT
CGACGTGCGCGCGACTAACGTCAAGCTGAACGGCAGCGCGTCGCTGAAGCTCGACGGACAGTTGATCCAGCTAGGCTGA