Detailed information of component protein


Summary

Component ID T6CP001069
Component protein type TssF
T6SS ID (Type) T6SS00113 experimental (Type i1)
Strain Burkholderia thailandensis E264
Replicon chromosome II
Sequence Protein sequence (581 a.a.); Nucleotide sequence (1743 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[4] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
[5] Schwarz S, Singh P, Robertson JD, et al. VgrG-5 is a Burkholderia type VI secretion system-exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 2014 Apr;82(4):1445-52. doi: 10.1128/IAI.01368-13. Epub 2014 Jan 22. PMID: 24452686
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BTH_RS04495
Coordinate (Strand) 1016371..1018113 (-)
NCBI ID WP_009896193.1
RefSeq NC_007650
Uniprot ID Q2T6Y6_BURTA
KEGG ID bte:BTH_II0866
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 3.4e-148 4..577

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-580 MATTTLNRYYEDELVRLRELAAEFGRAHPLLAPMLGAPSGDPDVERLLEGVAFLTGLARQKLDDGLPELVQALANLLFPHSLCPVPAATLIAFEPRGALRERAVVAAGTEIESMPVDGTACRFRTCGDLDVEPIALAGCRFVPPADGGPALRLDFEMLGIDASEWDATRIRLFVGGERLHASRLFAFLMQHVVSVDIASGPPELPGPRCTLGGRALRAAGFDDALLPCPERAFPGFRLLHEYFAYAEKFLFVELSGIERWRTARSGSQFSVRLALDCAPDWLPGIERDSFRLNVVAALNLFAHEAVPIQHEHRATDYRLQPEGDAHGHCRIYSVDRVVGYRPGHPVDRHYVAFGAAGDGALTASYRLIRRAALDGRGHDVHLALAYPPGEALAAETLSIGLSCTNGTLPARLRIGDVCRPTDSSPERFAFANIAPVSPPLDPPLGEPLLWRTIGHLALNFLSLGNVDNLKQMLALHAFGERGDAARAQADRRRIDGIEAVDVRAETRIVGERMLSGQHVALRCRAHAFGGAGELYLFGCVLERFLAEYAALNTYTRVEIDASPDDGRFAWPPRMGAQCLL



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9832 - -



  Protein sequence: 581 a.a.    

>T6CP001069 NC_007650:c1018113-1016371 [Burkholderia thailandensis E264] [TssF]
MATTTLNRYYEDELVRLRELAAEFGRAHPLLAPMLGAPSGDPDVERLLEGVAFLTGLARQKLDDGLPELVQALANLLFPH
SLCPVPAATLIAFEPRGALRERAVVAAGTEIESMPVDGTACRFRTCGDLDVEPIALAGCRFVPPADGGPALRLDFEMLGI
DASEWDATRIRLFVGGERLHASRLFAFLMQHVVSVDIASGPPELPGPRCTLGGRALRAAGFDDALLPCPERAFPGFRLLH
EYFAYAEKFLFVELSGIERWRTARSGSQFSVRLALDCAPDWLPGIERDSFRLNVVAALNLFAHEAVPIQHEHRATDYRLQ
PEGDAHGHCRIYSVDRVVGYRPGHPVDRHYVAFGAAGDGALTASYRLIRRAALDGRGHDVHLALAYPPGEALAAETLSIG
LSCTNGTLPARLRIGDVCRPTDSSPERFAFANIAPVSPPLDPPLGEPLLWRTIGHLALNFLSLGNVDNLKQMLALHAFGE
RGDAARAQADRRRIDGIEAVDVRAETRIVGERMLSGQHVALRCRAHAFGGAGELYLFGCVLERFLAEYAALNTYTRVEID
ASPDDGRFAWPPRMGAQCLL*

  Nucleotide sequence: 1743 bp    

>T6CP001069 NC_007650:c1018113-1016371 [Burkholderia thailandensis E264] [TssF]
ATGGCCACCACCACGCTGAATCGCTATTACGAGGACGAACTCGTCCGCCTGCGCGAGCTCGCCGCCGAGTTCGGCCGCGC
GCACCCGCTGCTCGCGCCCATGCTCGGCGCGCCGTCGGGCGACCCGGACGTCGAGCGGCTGCTCGAAGGCGTCGCGTTCC
TGACGGGGCTCGCGCGGCAGAAGCTCGACGACGGCCTGCCGGAACTCGTGCAGGCCCTCGCGAACCTACTGTTTCCGCAC
TCGCTGTGCCCGGTTCCGGCGGCGACGCTGATCGCGTTCGAGCCGCGCGGCGCGTTGCGCGAGCGCGCGGTGGTGGCGGC
CGGCACCGAGATCGAATCGATGCCTGTCGACGGCACGGCCTGCCGGTTCCGCACGTGCGGCGACCTCGACGTCGAGCCGA
TCGCGCTCGCCGGCTGCCGTTTCGTGCCGCCGGCGGACGGCGGGCCCGCGCTGCGGCTCGACTTCGAGATGCTCGGCATC
GACGCGAGCGAATGGGATGCGACGCGCATCCGCCTGTTCGTCGGCGGCGAGCGGCTGCACGCGAGCCGGCTGTTCGCGTT
CCTGATGCAGCACGTCGTATCGGTCGACATCGCGAGCGGCCCGCCGGAGCTGCCCGGCCCCCGCTGCACGCTCGGCGGCC
GCGCGCTGCGCGCGGCGGGCTTCGACGACGCGCTGCTGCCCTGCCCCGAGCGCGCGTTCCCCGGCTTCAGGCTGCTGCAC
GAATATTTCGCATACGCCGAGAAATTCCTGTTCGTCGAGCTGAGCGGCATCGAGCGCTGGCGCACCGCGCGCTCGGGCTC
GCAGTTCAGCGTGCGGCTCGCGCTCGATTGCGCGCCCGACTGGCTGCCCGGCATCGAGCGCGACAGCTTCCGGCTGAACG
TCGTCGCCGCGCTGAACCTGTTCGCGCACGAGGCGGTGCCGATCCAGCACGAGCACCGCGCGACCGACTACCGGCTGCAA
CCCGAAGGCGACGCGCACGGCCACTGCCGGATCTACTCGGTCGATCGCGTGGTCGGCTATCGCCCCGGCCATCCGGTCGA
TCGGCATTACGTCGCGTTCGGCGCGGCGGGCGACGGCGCGCTCACCGCGAGCTACCGGCTGATTCGCCGCGCGGCGCTCG
ACGGACGCGGGCACGACGTCCATCTGGCGCTCGCGTATCCGCCCGGCGAGGCGCTCGCGGCGGAAACGCTGTCGATCGGC
CTGTCGTGCACGAACGGCACGTTGCCCGCGCGCCTGAGGATCGGCGACGTGTGCCGCCCGACCGACAGCTCGCCCGAGCG
CTTCGCGTTCGCGAACATCGCGCCCGTGAGCCCGCCGCTCGACCCGCCGCTCGGCGAGCCGCTGCTGTGGCGAACCATCG
GCCATCTCGCGCTGAATTTCCTGTCGCTCGGCAACGTCGACAACCTGAAGCAGATGCTCGCGCTGCATGCGTTCGGCGAG
CGCGGCGACGCTGCGCGCGCGCAAGCCGACCGCCGCCGCATCGACGGCATCGAAGCGGTCGACGTGCGCGCCGAGACGCG
GATCGTCGGCGAGCGGATGCTGAGCGGCCAGCACGTCGCGCTGCGCTGCCGCGCTCACGCGTTCGGCGGCGCGGGCGAGC
TGTATCTGTTCGGCTGCGTGCTCGAGCGCTTCCTGGCCGAATACGCGGCGCTCAACACGTACACGCGAGTCGAGATCGAC
GCGTCGCCTGATGACGGGCGCTTCGCATGGCCGCCGCGGATGGGGGCGCAATGCCTGCTCTAG