Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RAM32_RS11620 Genome accession   NZ_CP132361
Coordinates   2360949..2361923 (-) Length   324 a.a.
NCBI ID   WP_000697219.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SA56     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2355949..2366923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM32_RS11580 (RAM32_11570) gcvT 2356450..2357541 (-) 1092 WP_000093345.1 glycine cleavage system aminomethyltransferase GcvT -
  RAM32_RS11585 (RAM32_11575) - 2357700..2358224 (-) 525 WP_001015121.1 shikimate kinase -
  RAM32_RS11590 (RAM32_11580) - 2358214..2358360 (-) 147 WP_001789879.1 hypothetical protein -
  RAM32_RS11595 (RAM32_11585) comGF 2358457..2358954 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM32_RS11600 (RAM32_11590) comGE 2358872..2359171 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  RAM32_RS11605 (RAM32_11595) comGD 2359158..2359604 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM32_RS11610 (RAM32_11600) comGC 2359582..2359893 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM32_RS11615 (RAM32_11605) comGB 2359907..2360977 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM32_RS11620 (RAM32_11610) comGA 2360949..2361923 (-) 975 WP_000697219.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM32_RS11625 (RAM32_11615) - 2361975..2362598 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RAM32_RS11630 (RAM32_11620) - 2362595..2362924 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM32_RS11635 (RAM32_11625) - 2362924..2363910 (-) 987 WP_000161315.1 ROK family glucokinase -
  RAM32_RS11640 (RAM32_11630) - 2363907..2364110 (-) 204 WP_000087561.1 YqgQ family protein -
  RAM32_RS11645 (RAM32_11635) - 2364091..2365554 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  RAM32_RS11650 (RAM32_11640) - 2365566..2366105 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  RAM32_RS11655 (RAM32_11645) rpmG 2366314..2366463 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36906.97 Da        Isoelectric Point: 8.8524

>NTDB_id=868458 RAM32_RS11620 WP_000697219.1 2360949..2361923(-) (comGA) [Staphylococcus aureus strain UNC_SA56]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIHASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=868458 RAM32_RS11620 WP_000697219.1 2360949..2361923(-) (comGA) [Staphylococcus aureus strain UNC_SA56]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACATGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997