Detailed information    

insolico Bioinformatically predicted

Overview


Name   recA   Type   Machinery gene
Locus tag   CDIF840101_RS07285 Genome accession   NZ_CP132146
Coordinates   1493775..1494821 (+) Length   348 a.a.
NCBI ID   WP_003428218.1    Uniprot ID   A0A031WFI0
Organism   Clostridioides difficile strain SC084-01-01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1465892..1533377 1493775..1494821 within 0


Gene organization within MGE regions


Location: 1465892..1533377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDIF840101_RS07170 (CDIF840101_14270) rimP 1470134..1470595 (+) 462 WP_003438303.1 ribosome maturation factor RimP -
  CDIF840101_RS07175 (CDIF840101_14280) nusA 1470625..1471779 (+) 1155 WP_003428238.1 transcription termination factor NusA -
  CDIF840101_RS07180 (CDIF840101_14290) rnpM 1471786..1472067 (+) 282 WP_003419469.1 RNase P modulator RnpM -
  CDIF840101_RS07185 (CDIF840101_14300) - 1472057..1472368 (+) 312 WP_003419470.1 L7Ae/L30e/S12e/Gadd45 family ribosomal protein -
  CDIF840101_RS07190 (CDIF840101_14310) infB 1472390..1474330 (+) 1941 WP_003438306.1 translation initiation factor IF-2 -
  CDIF840101_RS07195 (CDIF840101_14320) rbfA 1474373..1474753 (+) 381 WP_003428234.1 30S ribosome-binding factor RbfA -
  CDIF840101_RS07200 (CDIF840101_14330) - 1474734..1475411 (+) 678 Protein_1331 DHH family phosphoesterase -
  CDIF840101_RS07205 (CDIF840101_14340) tnpB 1476060..1477178 (+) 1119 WP_032548186.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  CDIF840101_RS07210 (CDIF840101_14350) - 1477296..1477601 (+) 306 Protein_1333 DHH family phosphoesterase -
  CDIF840101_RS07215 (CDIF840101_14360) truB 1477614..1478519 (+) 906 WP_009896221.1 tRNA pseudouridine(55) synthase TruB -
  CDIF840101_RS07220 (CDIF840101_14370) - 1478650..1479579 (+) 930 WP_003438310.1 bifunctional riboflavin kinase/FAD synthetase -
  CDIF840101_RS07225 (CDIF840101_14380) rpsO 1479683..1479940 (+) 258 WP_003419491.1 30S ribosomal protein S15 -
  CDIF840101_RS07230 (CDIF840101_14390) - 1480180..1481025 (+) 846 WP_009889099.1 DegV family protein -
  CDIF840101_RS07235 (CDIF840101_14400) pnp 1481150..1483261 (+) 2112 WP_003438316.1 polyribonucleotide nucleotidyltransferase -
  CDIF840101_RS07240 (CDIF840101_14410) - 1483527..1484270 (+) 744 WP_003438319.1 polysaccharide deacetylase family protein -
  CDIF840101_RS07245 (CDIF840101_14420) - 1484428..1485675 (+) 1248 WP_003438321.1 M16 family metallopeptidase -
  CDIF840101_RS07250 (CDIF840101_14430) - 1485710..1485955 (+) 246 WP_003419503.1 YlmC/YmxH family sporulation protein -
  CDIF840101_RS07255 (CDIF840101_14440) dapG 1485965..1487188 (+) 1224 WP_003438324.1 aspartate kinase -
  CDIF840101_RS07260 (CDIF840101_14450) - 1487301..1488035 (+) 735 WP_003428224.1 ClpP family protease -
  CDIF840101_RS07265 (CDIF840101_14460) - 1488158..1490569 (+) 2412 WP_009896226.1 FtsK/SpoIIIE family DNA translocase -
  CDIF840101_RS07270 (CDIF840101_14470) mnmH 1490572..1491630 (+) 1059 WP_003433322.1 tRNA 2-selenouridine(34) synthase MnmH -
  CDIF840101_RS07275 (CDIF840101_14480) rimO 1491624..1492958 (+) 1335 WP_003433320.1 30S ribosomal protein S12 methylthiotransferase RimO -
  CDIF840101_RS07280 (CDIF840101_14490) pgsA 1492945..1493487 (+) 543 WP_009889106.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  CDIF840101_RS07285 (CDIF840101_14500) recA 1493775..1494821 (+) 1047 WP_003428218.1 recombinase RecA Machinery gene
  CDIF840101_RS07290 (CDIF840101_14510) rny 1494997..1496538 (+) 1542 WP_003428216.1 ribonuclease Y -
  CDIF840101_RS07295 (CDIF840101_14520) - 1497173..1498954 (+) 1782 WP_003438344.1 UvrD-helicase domain-containing protein -
  CDIF840101_RS07300 (CDIF840101_14530) lepB 1499036..1499566 (-) 531 WP_009896231.1 signal peptidase I -
  CDIF840101_RS07305 (CDIF840101_14540) - 1499606..1500430 (-) 825 WP_003438350.1 undecaprenyl-diphosphate phosphatase -
  CDIF840101_RS07310 (CDIF840101_14550) - 1500687..1501595 (+) 909 WP_009889110.1 tyrosine-type recombinase/integrase -
  CDIF840101_RS07315 (CDIF840101_14560) purB 1501739..1503184 (+) 1446 WP_009896234.1 adenylosuccinate lyase -
  CDIF840101_RS07320 (CDIF840101_14570) - 1503496..1503948 (+) 453 WP_003419561.1 MarR family winged helix-turn-helix transcriptional regulator -
  CDIF840101_RS07325 (CDIF840101_14580) - 1504283..1505479 (-) 1197 WP_003438357.1 pyridoxal phosphate-dependent aminotransferase -
  CDIF840101_RS07330 (CDIF840101_14590) galT 1505864..1506814 (+) 951 WP_003438360.1 galactose-1-phosphate uridylyltransferase -
  CDIF840101_RS07335 (CDIF840101_14600) - 1506975..1507727 (+) 753 WP_003438367.1 exodeoxyribonuclease III -
  CDIF840101_RS07340 (CDIF840101_14610) - 1507819..1508595 (-) 777 WP_009896237.1 3'-5' exonuclease -
  CDIF840101_RS07345 (CDIF840101_14620) - 1508910..1510808 (+) 1899 WP_003428202.1 glutamine synthetase -
  CDIF840101_RS07350 (CDIF840101_14630) - 1510936..1512057 (-) 1122 WP_003438374.1 MGDG synthase family glycosyltransferase -
  CDIF840101_RS07355 (CDIF840101_14640) - 1512256..1512588 (+) 333 WP_009889116.1 PadR family transcriptional regulator -
  CDIF840101_RS07360 (CDIF840101_14650) - 1512575..1513657 (+) 1083 WP_011861182.1 DUF1700 domain-containing protein -
  CDIF840101_RS07365 (CDIF840101_14660) - 1513650..1514720 (+) 1071 WP_009896243.1 DUF4097 family beta strand repeat-containing protein -
  CDIF840101_RS07370 (CDIF840101_14670) - 1514822..1515358 (+) 537 WP_003428196.1 lipoprotein -
  CDIF840101_RS07375 (CDIF840101_14680) - 1515474..1516181 (+) 708 WP_009896245.1 lantibiotic protection ABC transporter ATP-binding protein -
  CDIF840101_RS07380 (CDIF840101_14690) - 1516183..1516899 (+) 717 WP_009896246.1 lantibiotic immunity ABC transporter MutE/EpiE family permease subunit -
  CDIF840101_RS07385 (CDIF840101_14700) - 1516901..1517659 (+) 759 WP_009896249.1 lantibiotic immunity ABC transporter MutG family permease subunit -
  CDIF840101_RS07390 (CDIF840101_14710) - 1517794..1519182 (+) 1389 WP_009896251.1 two-component system sensor histidine kinase -
  CDIF840101_RS07395 (CDIF840101_14720) - 1519258..1520079 (-) 822 WP_009896253.1 pyridoxamine kinase -
  CDIF840101_RS07400 (CDIF840101_14730) - 1520330..1521484 (+) 1155 WP_009902426.1 amidase domain-containing protein -
  CDIF840101_RS07405 (CDIF840101_14740) - 1521667..1521867 (+) 201 WP_003419624.1 cold-shock protein -
  CDIF840101_RS07410 (CDIF840101_14750) - 1522288..1522716 (+) 429 WP_009889129.1 CBS domain-containing protein -
  CDIF840101_RS07415 (CDIF840101_14760) - 1522859..1523614 (-) 756 WP_009889131.1 peptidylprolyl isomerase -
  CDIF840101_RS07420 (CDIF840101_14780) - 1524315..1524821 (-) 507 WP_003428186.1 ImmA/IrrE family metallo-endopeptidase -
  CDIF840101_RS07425 (CDIF840101_14790) - 1524903..1525223 (-) 321 WP_003428185.1 XRE family transcriptional regulator -
  CDIF840101_RS07430 (CDIF840101_14800) - 1525392..1525589 (+) 198 WP_003419641.1 helix-turn-helix transcriptional regulator -
  CDIF840101_RS07435 (CDIF840101_14810) - 1525694..1526134 (+) 441 WP_003428183.1 hypothetical protein -
  CDIF840101_RS07440 (CDIF840101_14820) - 1526383..1526826 (+) 444 WP_009896258.1 phage tail terminator family protein -
  CDIF840101_RS07445 (CDIF840101_14830) - 1526831..1527895 (+) 1065 WP_009896260.1 phage tail sheath subtilisin-like domain-containing protein -
  CDIF840101_RS07450 (CDIF840101_14840) - 1527909..1528337 (+) 429 WP_009896262.1 phage tail tube protein -
  CDIF840101_RS07455 (CDIF840101_14850) - 1528451..1528897 (+) 447 WP_009889139.1 phage tail assembly chaperone -
  CDIF840101_RS07460 - 1528924..1529091 (+) 168 WP_009896264.1 hypothetical protein -
  CDIF840101_RS07465 (CDIF840101_14860) - 1529091..1531421 (+) 2331 WP_009896266.1 hypothetical protein -
  CDIF840101_RS07470 (CDIF840101_14870) - 1531421..1531843 (+) 423 WP_009896267.1 hypothetical protein -

Sequence


Protein


Download         Length: 348 a.a.        Molecular weight: 37377.68 Da        Isoelectric Point: 4.8236

>NTDB_id=867304 CDIF840101_RS07285 WP_003428218.1 1493775..1494821(+) (recA) [Clostridioides difficile strain SC084-01-01]
MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTGAIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSC
VASAQKDGGIAAFIDAEHALDPVYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKFYSSVRLDVRKIDTIKQGDKVIG
SRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLLDIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEI
DEKVRAFYNLNEEHEESGTSVSKEIVEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=867304 CDIF840101_RS07285 WP_003428218.1 1493775..1494821(+) (recA) [Clostridioides difficile strain SC084-01-01]
ATGAGTGTAGATCAAGAAAAATTAAAAGCGTTGAATGAAGCTTTAGGTAAAATTGAAAAAGATTTTGGTAAAGGTTCAGT
AATGAAATTGGGAGAAGCAACATCTATGTCTATAGATGTTATATCAACAGGAGCGATTGGTTTAGACATAGCTATTGGTA
TAGGAGGTCTACCTAGAGGGAGAATAGTTGAAGTATATGGTCCAGAATCTTCTGGTAAGACTACTGTTGCGCTTAGTTGT
GTAGCATCAGCTCAAAAAGATGGAGGAATAGCTGCATTTATAGATGCAGAACATGCACTTGACCCAGTATATGCAAAAGC
TTTGGGTGTGGATGTTGATAACCTAATAATATCTCAACCAGATACAGGTGAACAGGCTTTAGAGATAGCAGAGGCATTGA
TAAGAAGTGGAGCGATAGATATAATAGTAATAGACTCAGTAGCAGCATTAGTTCCAAAGGCTGAAATAGATGGAGATATG
GGTGATTCTCATGTAGGTTTACAAGCTAGACTTATGTCACAAGCACTTAGAAAGTTAACTGGTTCAATTAAAAAATCAAA
TTGTGTTGCTATATTTATAAACCAGTTAAGAGAGAAAGTAGGAATAATGTTTGGAAACCCAGAAACTACTACTGGAGGAC
GTGCACTAAAATTCTATTCATCAGTTAGATTGGATGTTAGAAAAATAGATACAATAAAACAAGGTGATAAAGTTATAGGT
AGTAGAACTAGAGTTAAAGTTGTTAAAAACAAAGTAGCACCACCATTTAAGCAAGCTGAATTTGATATAATGTATGGAGA
AGGGATTTCAAAAATTGGAGACCTTTTAGATATAGCTGCTGATGTAGATATAGTTAAAAAATCAGGTTCATGGTATAGTT
ACAATGATACTAAACTTGGACAAGGAAGAGAAAATGTTAAAAAATTCTTGGAAGATAATTTAGATTTAACTACTGAAATA
GATGAGAAAGTTAGAGCATTTTACAATTTAAATGAAGAACATGAAGAATCAGGTACTTCAGTATCAAAAGAAATTGTAGA
AGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A031WFI0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Streptococcus mutans UA159

66.762

100

0.67

  recA Streptococcus mitis SK321

67.456

97.126

0.655

  recA Streptococcus pneumoniae Rx1

65.507

99.138

0.649

  recA Streptococcus pneumoniae R6

65.507

99.138

0.649

  recA Streptococcus pneumoniae D39

65.507

99.138

0.649

  recA Streptococcus pneumoniae TIGR4

65.507

99.138

0.649

  recA Streptococcus mitis NCTC 12261

68.085

94.54

0.644

  recA Bacillus subtilis subsp. subtilis str. 168

69.565

92.529

0.644

  recA Lactococcus lactis subsp. cremoris KW2

65.569

95.977

0.629

  recA Streptococcus pyogenes NZ131

66.972

93.966

0.629

  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

65.758

94.828

0.624

  recA Latilactobacillus sakei subsp. sakei 23K

64.955

95.115

0.618

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae MS11

66.049

93.103

0.615

  recA Neisseria gonorrhoeae strain FA1090

66.049

93.103

0.615

  recA Acinetobacter baylyi ADP1

64.396

92.816

0.598

  recA Pseudomonas stutzeri DSM 10701

63.889

93.103

0.595

  recA Helicobacter pylori 26695

61.976

95.977

0.595

  recA Helicobacter pylori strain NCTC11637

61.976

95.977

0.595

  recA Acinetobacter baumannii D1279779

63.777

92.816

0.592

  recA Vibrio cholerae O1 biovar El Tor strain E7946

62.048

95.402

0.592

  recA Vibrio cholerae strain A1552

62.048

95.402

0.592

  recA Glaesserella parasuis strain SC1401

64.174

92.241

0.592

  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

63.272

93.103

0.589

  recA Ralstonia pseudosolanacearum GMI1000

66.558

88.506

0.589

  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

60.681

92.816

0.563