Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EUCAG14_RS02030 Genome accession   NZ_CP132137
Coordinates   499070..500119 (+) Length   349 a.a.
NCBI ID   WP_148429888.1    Uniprot ID   -
Organism   Eubacterium callanderi strain DSM 107592     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494070..505119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUCAG14_RS02010 (EUCAG14_04040) - 494142..494564 (-) 423 WP_013382402.1 Hsp20/alpha crystallin family protein -
  EUCAG14_RS02015 (EUCAG14_04050) - 494886..496736 (+) 1851 WP_148429892.1 diguanylate cyclase domain-containing protein -
  EUCAG14_RS02020 (EUCAG14_04060) - 496738..497253 (+) 516 WP_013382400.1 type 1 glutamine amidotransferase domain-containing protein -
  EUCAG14_RS02025 (EUCAG14_04070) - 497373..499049 (+) 1677 WP_148429890.1 GspE/PulE family protein -
  EUCAG14_RS02030 (EUCAG14_04080) pilT 499070..500119 (+) 1050 WP_148429888.1 type IV pilus twitching motility protein PilT Machinery gene
  EUCAG14_RS02035 (EUCAG14_04090) - 500138..503254 (+) 3117 WP_148429886.1 EAL domain-containing protein -
  EUCAG14_RS02040 (EUCAG14_04100) - 503315..504595 (+) 1281 WP_148429885.1 O-antigen ligase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38655.21 Da        Isoelectric Point: 6.1805

>NTDB_id=867211 EUCAG14_RS02030 WP_148429888.1 499070..500119(+) (pilT) [Eubacterium callanderi strain DSM 107592]
MPTILDLIDYGRQTGCSDIHLTADLPPVFRSNGVLVKGPFEMSKREIGEMIIGMLSEMNREKIKRGEDADFTFVTPEGLR
QRVNVYRQQNELCAAVRLLNDTIPTFEELGLPPIIRKLAAEPRGLILITGPTGSGKSTTLAAMIDYINVNFAKHIITIED
PVEYRHYHKLSMIHQREVGVDVSSFADALRSSLREDPDVILVREMRDYETISAAVTAAETGHLVLSTLHTIGAANTVDRI
IDVFPPHSQQQIRTQLASTLKGVVTQQLVPLASGEGRMAALEVMTGTDAVLNLIRENKTHQLSSAMQTGSRDGMNTLNSH
LAKLVKEGKIGFDTGLQWSSDKNEFNQYF

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=867211 EUCAG14_RS02030 WP_148429888.1 499070..500119(+) (pilT) [Eubacterium callanderi strain DSM 107592]
ATGCCAACCATCTTAGACCTAATCGACTACGGCCGCCAGACTGGCTGTTCGGATATTCATTTGACGGCAGATCTGCCGCC
GGTATTCAGGAGCAACGGGGTGCTTGTCAAAGGCCCCTTTGAAATGAGCAAAAGGGAAATCGGAGAGATGATCATCGGGA
TGCTCAGTGAGATGAACCGTGAGAAAATCAAGCGGGGAGAGGACGCGGATTTTACTTTTGTCACGCCAGAGGGGCTCAGA
CAGAGGGTTAACGTGTACCGCCAGCAGAATGAGCTCTGCGCTGCCGTTCGCCTGCTCAACGACACCATTCCTACCTTTGA
GGAGCTGGGCCTGCCGCCCATTATCCGCAAGCTGGCCGCTGAGCCGAGAGGGCTGATCCTGATCACCGGGCCGACCGGGA
GTGGGAAATCCACCACACTGGCAGCTATGATCGACTACATCAACGTCAATTTTGCCAAGCATATCATCACCATTGAGGAC
CCGGTGGAATACCGCCATTACCATAAACTGAGCATGATCCACCAGCGTGAGGTGGGAGTGGATGTATCCTCCTTTGCCGA
CGCGCTACGCTCATCCCTGCGTGAGGACCCCGACGTCATTCTGGTAAGGGAAATGCGTGACTACGAAACCATCTCGGCCG
CTGTAACCGCCGCTGAAACCGGGCATCTCGTGCTGTCTACTCTGCACACCATCGGTGCTGCCAACACAGTAGACCGTATT
ATCGACGTGTTCCCGCCCCACAGCCAGCAGCAGATACGGACACAGCTCGCCAGCACCCTGAAGGGTGTTGTGACACAGCA
GCTTGTGCCTCTGGCTTCCGGCGAAGGCCGGATGGCCGCCCTTGAGGTGATGACAGGCACCGACGCAGTGCTCAACCTTA
TCCGTGAAAACAAAACCCATCAGCTTTCCTCTGCCATGCAGACCGGCAGCCGGGACGGCATGAACACCCTGAACAGCCAC
CTGGCCAAGCTGGTGAAGGAAGGAAAGATCGGCTTTGATACCGGTCTCCAGTGGTCGTCAGACAAAAACGAGTTTAACCA
ATACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.563

98.281

0.487
  pilT Acinetobacter baylyi ADP1

46.064

98.281

0.453
  pilT Neisseria meningitidis 8013

47.72

94.269

0.45
  pilT Legionella pneumophila strain Lp02

45.217

98.854

0.447
  pilT Legionella pneumophila strain ERS1305867

45.217

98.854

0.447
  pilT Neisseria gonorrhoeae MS11

47.416

94.269

0.447
  pilT Acinetobacter nosocomialis M2

44.606

98.281

0.438
  pilT Acinetobacter baumannii D1279779

44.606

98.281

0.438
  pilT Acinetobacter baumannii strain A118

44.606

98.281

0.438
  pilT Pseudomonas aeruginosa PAK

46.061

94.556

0.436
  pilT Pseudomonas stutzeri DSM 10701

44.315

98.281

0.436
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.427

93.983

0.427
  pilT Vibrio cholerae strain A1552

45.427

93.983

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.597

95.989

0.39