Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PAK23_RS03900 Genome accession   NZ_CP115817
Coordinates   846445..846621 (+) Length   58 a.a.
NCBI ID   WP_276488429.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain GD22SC3150TT     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 841445..851621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAK23_RS03875 pilD 841692..842558 (-) 867 WP_276488424.1 A24 family peptidase Machinery gene
  PAK23_RS03880 pilC 842562..843782 (-) 1221 WP_276488425.1 type II secretion system F family protein Machinery gene
  PAK23_RS03885 pilB 843786..845483 (-) 1698 WP_276488426.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PAK23_RS03890 - 845715..845900 (+) 186 WP_276488427.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PAK23_RS03895 - 845916..846383 (+) 468 WP_276488428.1 hypothetical protein -
  PAK23_RS03900 pilA 846445..846621 (+) 177 WP_276488429.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PAK23_RS03905 - 846627..847442 (+) 816 WP_276488430.1 hypothetical protein -
  PAK23_RS03910 - 847465..848283 (+) 819 WP_276488431.1 hypothetical protein -
  PAK23_RS03915 - 848280..849191 (+) 912 WP_276488432.1 ABC transporter ATP-binding protein -
  PAK23_RS03920 nadC 849241..850089 (-) 849 WP_276488433.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 58 a.a.        Molecular weight: 6215.30 Da        Isoelectric Point: 10.0920

>NTDB_id=773804 PAK23_RS03900 WP_276488429.1 846445..846621(+) (pilA) [Pseudomonas mendocina strain GD22SC3150TT]
MRSKGFTLIELMVVVAIVGVLVAIAMPQFNEYRARSNDTVAAADAKTAISVFASDKAR

Nucleotide


Download         Length: 177 bp        

>NTDB_id=773804 PAK23_RS03900 WP_276488429.1 846445..846621(+) (pilA) [Pseudomonas mendocina strain GD22SC3150TT]
ATGAGATCCAAAGGCTTTACTCTGATTGAGCTGATGGTCGTCGTGGCGATTGTCGGAGTTTTGGTGGCAATCGCAATGCC
TCAATTTAATGAGTACCGTGCTCGATCAAATGATACGGTGGCAGCCGCGGATGCTAAAACCGCAATCTCCGTGTTTGCCT
CGGACAAGGCTCGTTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

58.491

91.379

0.534
  pilA2 Legionella pneumophila str. Paris

58.491

91.379

0.534
  pilA Ralstonia pseudosolanacearum GMI1000

54.545

94.828

0.517
  comP Acinetobacter baylyi ADP1

56

86.207

0.483
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

50

96.552

0.483
  pilA Pseudomonas aeruginosa PAK

65

68.966

0.448
  pilA/pilA1 Eikenella corrodens VA1

45.614

98.276

0.448
  pilA Vibrio parahaemolyticus RIMD 2210633

51.02

84.483

0.431
  pilA/pilAII Pseudomonas stutzeri DSM 10701

48.98

84.483

0.414
  pilA/pilA4 Thermus thermophilus HB27

52.273

75.862

0.397
  pilA Acinetobacter baumannii strain A118

69.697

56.897

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

67.647

58.621

0.397
  pilA Acinetobacter nosocomialis M2

66.667

56.897

0.379
  pilA Vibrio cholerae strain A1552

62.857

60.345

0.379
  pilA Vibrio cholerae C6706

62.857

60.345

0.379
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

62.857

60.345

0.379
  comE Acinetobacter baylyi ADP1

61.765

58.621

0.362
  comF Acinetobacter baylyi ADP1

61.765

58.621

0.362
  pilE Neisseria gonorrhoeae strain FA1090

61.765

58.621

0.362
  pilE Neisseria gonorrhoeae MS11

63.636

56.897

0.362