NTDB

Overview

Name	pilA/pilA1	Type	Machinery gene
Locus tag	NHM04_RS14460	Genome accession	NZ_CP100350
Coordinates	3312686..3313108 (+)	Length	140 a.a.
NCBI ID	WP_305881949.1	Uniprot ID	-
Organism	Gilvimarinus sp. DA14
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3307686..3318108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NHM04_RS14440 (NHM04_14440)	pilD	3308524..3309381 (-)	858	WP_254264464.1	A24 family peptidase	Machinery gene
NHM04_RS14445 (NHM04_14445)	pilC	3309390..3310649 (-)	1260	WP_254264465.1	type II secretion system F family protein	Machinery gene
NHM04_RS14450 (NHM04_14450)	pilB	3310653..3312359 (-)	1707	WP_254264466.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NHM04_RS14460 (NHM04_14460)	pilA/pilA1	3312686..3313108 (+)	423	WP_305881949.1	pilin	Machinery gene
NHM04_RS14470 (NHM04_14470)	pilA/pilA1	3313174..3313587 (+)	414	WP_305881950.1	pilin	Machinery gene
NHM04_RS14475 (NHM04_14475)	-	3313662..3314027 (+)	366	WP_254264467.1	transposase	-
NHM04_RS14480 (NHM04_14480)	-	3313979..3314503 (+)	525	WP_254264468.1	hypothetical protein	-
NHM04_RS14485 (NHM04_14485)	nadC	3314572..3315435 (-)	864	WP_254264469.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

Download Length: 140 a.a. Molecular weight: 14205.12 Da Isoelectric Point: 4.2507

                                >NTDB_id=704709 NHM04_RS14460 WP_305881949.1 3312686..3313108(+) (pilA/pilA1) [Gilvimarinus sp. DA14]

MKKQQGFTLIELMIVVAIIGILAAIALPAYQDYTARSQATEALKISAGIQSDVGVFLANEGSLGISSAAATAAGDLEGKY

VSGVSVAEGTGIITVNFGSGALSSQSMTLTPYENTAGGQIAYWECTGLTNASHLPSTCAP

Nucleotide

Download Length: 423 bp

                                >NTDB_id=704709 NHM04_RS14460 WP_305881949.1 3312686..3313108(+) (pilA/pilA1) [Gilvimarinus sp. DA14]

ATGAAGAAGCAACAAGGTTTTACTTTGATTGAATTGATGATCGTAGTAGCGATTATCGGTATTCTGGCGGCGATTGCTTT

GCCTGCTTACCAAGATTACACTGCTCGTTCGCAGGCAACTGAAGCTTTAAAAATTAGTGCGGGAATTCAGTCTGACGTAG

GTGTATTTCTAGCCAACGAGGGCTCCCTGGGCATTAGTTCAGCCGCTGCAACTGCTGCTGGAGACCTTGAGGGTAAATAT

GTAAGTGGTGTTTCTGTGGCTGAGGGTACAGGTATTATCACTGTTAACTTCGGTAGCGGCGCCCTCTCTAGTCAAAGTAT

GACTTTGACTCCCTATGAAAATACTGCCGGTGGCCAAATTGCATACTGGGAATGTACTGGTTTGACTAATGCTTCGCATC

TGCCAAGCACTTGTGCTCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-138)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilA1	Eikenella corrodens VA1	51.656	100	0.557
pilA2	Legionella pneumophila strain ERS1305867	42	100	0.45
pilE	Neisseria gonorrhoeae strain FA1090	39.103	100	0.436
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40	100	0.429
pilA	Pseudomonas aeruginosa PAK	37.821	100	0.421
pilE	Neisseria gonorrhoeae MS11	37.821	100	0.421
pilA	Haemophilus influenzae Rd KW20	41.844	100	0.421
pilA	Haemophilus influenzae 86-028NP	41.135	100	0.414
pilA	Ralstonia pseudosolanacearum GMI1000	38.776	100	0.407
pilA	Acinetobacter baumannii strain A118	38.095	100	0.4
comP	Acinetobacter baylyi ADP1	37.162	100	0.393
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.568	99.286	0.393
pilA2	Legionella pneumophila str. Paris	39.568	99.286	0.393
pilA	Vibrio cholerae C6706	36.486	100	0.386
pilA	Vibrio cholerae strain A1552	36.486	100	0.386
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.486	100	0.386

Detailed information NTG0704709