Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   NHM04_RS14470 Genome accession   NZ_CP100350
Coordinates   3313174..3313587 (+) Length   137 a.a.
NCBI ID   WP_305881950.1    Uniprot ID   -
Organism   Gilvimarinus sp. DA14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3308174..3318587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHM04_RS14440 (NHM04_14440) pilD 3308524..3309381 (-) 858 WP_254264464.1 A24 family peptidase Machinery gene
  NHM04_RS14445 (NHM04_14445) pilC 3309390..3310649 (-) 1260 WP_254264465.1 type II secretion system F family protein Machinery gene
  NHM04_RS14450 (NHM04_14450) pilB 3310653..3312359 (-) 1707 WP_254264466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NHM04_RS14460 (NHM04_14460) pilA/pilA1 3312686..3313108 (+) 423 WP_305881949.1 pilin Machinery gene
  NHM04_RS14470 (NHM04_14470) pilA/pilA1 3313174..3313587 (+) 414 WP_305881950.1 pilin Machinery gene
  NHM04_RS14475 (NHM04_14475) - 3313662..3314027 (+) 366 WP_254264467.1 transposase -
  NHM04_RS14480 (NHM04_14480) - 3313979..3314503 (+) 525 WP_254264468.1 hypothetical protein -
  NHM04_RS14485 (NHM04_14485) nadC 3314572..3315435 (-) 864 WP_254264469.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NHM04_RS14490 (NHM04_14490) - 3315838..3318120 (+) 2283 WP_254264470.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14150.17 Da        Isoelectric Point: 4.6243

>NTDB_id=704710 NHM04_RS14470 WP_305881950.1 3313174..3313587(+) (pilA/pilA1) [Gilvimarinus sp. DA14]
MKHNGFTLLELMIVVAIIGIIASLAFPAMQTYMARSQAMEGIKGTAGLRASVAQYFAENASFPNGADLQNIEAGIEGKYY
SAGGVALASGVITVNFSAGLLSGEALTLEPTQNAAGNQISGWRCAGLDVSYLPGSCQ

Nucleotide


Download         Length: 414 bp        

>NTDB_id=704710 NHM04_RS14470 WP_305881950.1 3313174..3313587(+) (pilA/pilA1) [Gilvimarinus sp. DA14]
ATGAAACACAACGGCTTCACTCTATTAGAGTTAATGATTGTTGTGGCGATCATTGGCATTATCGCATCACTGGCTTTTCC
TGCTATGCAGACCTATATGGCTAGATCGCAGGCGATGGAAGGTATCAAAGGAACGGCGGGATTGCGGGCATCGGTAGCTC
AATATTTTGCCGAAAATGCGTCTTTTCCTAATGGCGCGGATCTCCAAAATATTGAGGCTGGTATTGAGGGTAAATATTAT
TCCGCTGGAGGTGTTGCTCTCGCATCTGGCGTAATTACGGTCAATTTTTCCGCTGGACTTTTGTCTGGTGAAGCTTTAAC
TTTAGAGCCTACTCAGAATGCCGCTGGAAACCAGATCTCAGGTTGGCGTTGCGCTGGGCTTGATGTCAGCTATTTACCTG
GTAGCTGTCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

45.946

100

0.496
  pilA2 Legionella pneumophila strain ERS1305867

33.784

100

0.365
  pilA2 Legionella pneumophila str. Paris

33.784

100

0.365
  pilA Acinetobacter nosocomialis M2

36.496

100

0.365