Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NHM04_RS14450 Genome accession   NZ_CP100350
Coordinates   3310653..3312359 (-) Length   568 a.a.
NCBI ID   WP_254264466.1    Uniprot ID   A0A9J7A6I4
Organism   Gilvimarinus sp. DA14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3305653..3317359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHM04_RS14425 (NHM04_14425) - 3305887..3306849 (+) 963 WP_254264461.1 Nudix family hydrolase -
  NHM04_RS14430 (NHM04_14430) yacG 3306893..3307081 (-) 189 WP_254264462.1 DNA gyrase inhibitor YacG -
  NHM04_RS14435 (NHM04_14435) coaE 3307111..3307719 (-) 609 WP_254264463.1 dephospho-CoA kinase -
  NHM04_RS14440 (NHM04_14440) pilD 3308524..3309381 (-) 858 WP_254264464.1 A24 family peptidase Machinery gene
  NHM04_RS14445 (NHM04_14445) pilC 3309390..3310649 (-) 1260 WP_254264465.1 type II secretion system F family protein Machinery gene
  NHM04_RS14450 (NHM04_14450) pilB 3310653..3312359 (-) 1707 WP_254264466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NHM04_RS14460 (NHM04_14460) pilA/pilA1 3312686..3313108 (+) 423 WP_305881949.1 pilin Machinery gene
  NHM04_RS14470 (NHM04_14470) pilA/pilA1 3313174..3313587 (+) 414 WP_305881950.1 pilin Machinery gene
  NHM04_RS14475 (NHM04_14475) - 3313662..3314027 (+) 366 WP_254264467.1 transposase -
  NHM04_RS14480 (NHM04_14480) - 3313979..3314503 (+) 525 WP_254264468.1 hypothetical protein -
  NHM04_RS14485 (NHM04_14485) nadC 3314572..3315435 (-) 864 WP_254264469.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62191.31 Da        Isoelectric Point: 4.7879

>NTDB_id=704708 NHM04_RS14450 WP_254264466.1 3310653..3312359(-) (pilB) [Gilvimarinus sp. DA14]
MAALSGLAKRLVMDGVLDETLAADGLAQANKQKLPFALYLVQNDILDARTVAQIAADEFGTPVFDIRAISPSALPQKLVD
PKLIEKHHALPLMRRGNRLFVGMVDPTNLHALDEIKFNTGINTEAVLVEADKLAPALEAYINANEESLSDALGGLDDEDL
DLQTDGGEVKEEDTTSEADEAPIVRFINKILLDAIKIGASDIHFEPYEKAYRVRFRTDGILREMSKPPINLATRLAARLK
VMSQMDISERRVPQDGRIKMKISKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDDQKQMYLEALEQPQGM
ILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVQMNTKVGLSFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCTKCRKPADDIPDEILSEEGFDD
IGIPREEFELYHPVGCDACLKGYKGRVGVYEVVRITPEISSLIMEGGNSLQIAKAAKAAGFNNLRVSALRKAAQGLTSLE
EANRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=704708 NHM04_RS14450 WP_254264466.1 3310653..3312359(-) (pilB) [Gilvimarinus sp. DA14]
ATGGCAGCACTCAGCGGATTGGCAAAACGATTGGTAATGGACGGGGTATTGGACGAGACCCTGGCCGCAGACGGCCTGGC
CCAGGCCAATAAACAAAAGCTGCCCTTTGCACTGTATTTAGTGCAAAACGATATTCTGGATGCCCGCACCGTAGCGCAAA
TAGCGGCTGATGAGTTTGGCACCCCGGTTTTTGATATTCGTGCCATCAGCCCCAGCGCCCTGCCACAAAAACTGGTAGAT
CCCAAGCTGATTGAAAAACACCACGCCCTGCCATTAATGCGCCGGGGTAACCGACTGTTTGTGGGCATGGTGGACCCTAC
CAACCTGCACGCGCTGGATGAAATTAAATTTAATACCGGCATTAATACCGAGGCGGTGCTGGTAGAGGCGGACAAGCTGG
CCCCCGCCCTTGAAGCTTATATAAATGCCAATGAAGAAAGCTTAAGCGACGCCCTGGGCGGCCTGGACGATGAAGACCTG
GACCTGCAAACCGACGGCGGCGAGGTTAAAGAGGAAGACACCACCAGCGAGGCCGACGAAGCGCCCATTGTGCGCTTTAT
TAATAAAATTCTGCTGGATGCAATAAAGATTGGCGCCTCGGATATTCACTTTGAGCCCTATGAAAAAGCCTACCGGGTAA
GGTTCCGTACCGATGGTATTTTGCGGGAAATGTCCAAACCGCCCATTAACCTGGCCACCCGCCTGGCGGCGCGTTTAAAG
GTTATGTCACAAATGGATATTTCCGAACGCCGGGTGCCGCAGGATGGCCGGATTAAGATGAAGATATCCAAAACCCGAGC
CATTGATTTTCGGGTAAATACCCTACCTACCCTGTTTGGCGAGAAAATTGTGCTGCGAATTTTGGACCCCAGCTCTGCCA
AGCTGGGTATTGACGCCCTGGGCTATGAGGACGACCAAAAACAGATGTACCTGGAGGCCCTGGAGCAGCCCCAGGGCATG
ATTCTAGTGACCGGGCCCACCGGTAGCGGCAAAACGGTCTCCCTTTATACCGGGCTTAATATTCTGAATACCGAAGAGCG
CAATATATCCACAGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATTAACCAGGTGCAAATGAACACCAAGGTAGGGC
TGAGCTTTGCCGAGGCGCTGCGCTCATTCCTGCGGCAAGACCCGGATATTGTGATGGTGGGGGAGATTCGCGACCTGGAA
ACCGCCGAAATTGCCATTAAGGCGGCGCAAACCGGGCACTTGGTACTCTCTACCCTGCACACCAATAGCGCACCAGAAAC
ACTGACCCGCCTTTTGAATATGGGCGTACCCGCCTTTAACGTGGCCACCAGTGTTAGCTTGATTATTGCACAGCGATTGG
CAAGGCGGCTGTGCACCAAATGCCGTAAACCGGCAGACGACATACCGGATGAGATTTTAAGCGAAGAAGGTTTTGACGAT
ATTGGCATACCGCGGGAAGAGTTTGAACTCTATCACCCGGTAGGCTGCGATGCCTGCTTAAAAGGTTATAAGGGACGGGT
TGGGGTATATGAAGTGGTTCGCATCACCCCTGAGATATCCTCCCTTATAATGGAAGGGGGGAATTCTCTGCAAATTGCCA
AAGCCGCTAAAGCCGCCGGGTTTAATAATTTGCGGGTTTCAGCATTACGAAAAGCTGCACAGGGCTTAACCAGCCTGGAA
GAAGCCAACCGAGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.092

100

0.611
  pilB Acinetobacter baylyi ADP1

59.019

100

0.593
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.563
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.296

0.507
  pilB Vibrio cholerae strain A1552

53.061

94.894

0.504
  pilB Vibrio campbellii strain DS40M4

50.444

99.12

0.5
  pilF Neisseria gonorrhoeae MS11

49.635

96.479

0.479
  pilB/pilB1 Synechocystis sp. PCC 6803

38.25

100

0.415
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

93.31

0.366