Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N015_RS25000 Genome accession   NZ_CP047265
Coordinates   5578313..5579377 (-) Length   354 a.a.
NCBI ID   WP_024689062.1    Uniprot ID   -
Organism   Pseudomonas asturiensis strain CC1524     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5573313..5584377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N015_RS24975 (N015_24975) aroK 5573927..5574445 (-) 519 WP_004882714.1 shikimate kinase AroK -
  N015_RS24980 (N015_24980) pilQ 5574450..5576549 (-) 2100 WP_024689066.1 type IV pilus secretin PilQ Machinery gene
  N015_RS24985 (N015_24985) - 5576597..5577124 (-) 528 WP_024689065.1 pilus assembly protein PilP -
  N015_RS24990 (N015_24990) pilO 5577121..5577744 (-) 624 WP_024689064.1 type 4a pilus biogenesis protein PilO -
  N015_RS24995 (N015_24995) - 5577741..5578313 (-) 573 WP_024689063.1 PilN domain-containing protein -
  N015_RS25000 (N015_25000) pilM 5578313..5579377 (-) 1065 WP_024689062.1 pilus assembly protein PilM Machinery gene
  N015_RS25005 (N015_25005) - 5579587..5582028 (+) 2442 WP_419198400.1 penicillin-binding protein 1A -
  N015_RS25010 (N015_25010) - 5583012..5583467 (+) 456 WP_024685741.1 YkgJ family cysteine cluster protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38160.57 Da        Isoelectric Point: 4.7049

>NTDB_id=411935 N015_RS25000 WP_024689062.1 5578313..5579377(-) (pilM) [Pseudomonas asturiensis strain CC1524]
MFELFSKKANTLLGIDISSTSVKLLELSRSGNRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVIVKAKTSARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=411935 N015_RS25000 WP_024689062.1 5578313..5579377(-) (pilM) [Pseudomonas asturiensis strain CC1524]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTTGAATT
GAGTCGCTCCGGAAACCGCTACAAAGTCGAGTCTTACGCCGTCGAGCCGCTGCCTGCCAATGCCGTGGTCGAGAAGAACA
TTGCCGAGCTCGAAGGGGTCGGCCAGGCGCTGTCACGGGTTATCGTCAAGGCCAAGACCAGCGCCAGAATCGTGGCGGTG
GCCGTGGCAGGTTCCGCGGTCATCACCAAGACCATCGAGATGGATGCCGGCCTGTCGGACGACGACATGGAGAACCAGCT
CAAGCTCGAAGCCGACCAATACATTCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAGGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGTGTCGAGGTGCTGCTGGCGGCCTGTCGCAAGGAGAACGTCGAAGTTCGCGAGGCCGCGCTGGCATTG
GCCGGGCTGACCGCGCGCGTCGTCGACGTGGAGGCTTATGCGCTGGAACGTTCGTTCGGCCTGTTGGCGACGCAATTGGG
CAATGGCCATGACGAGCTGACCGTCGCTGTCGTCGACATCGGCGCGACCATGACCACCCTGAGCGTACTGCACCACGGCC
GCATCATTTATACCCGCGAGCAACTGTTTGGCGGGCGACAGCTGACGGATGAGATCCAGCGGCGCTATGGCCTCTCGATG
GAAGAAGCCGGCCTTGCGAAAAAGCAGGGCGGCCTGCCTGACGACTACGTCAGCGAGGTGCTGGAACCTTTCAAGGATGC
GTTGGTGCAACAGGTCTCGCGTTCCCTGCAGTTCTTCTTCGCGGCAGGTCAGTACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTTGAACATTTGATTCAGCGTCGTCTTGGCACGCCGACCCAGGTAGCCAACCCG
TTTGCTGACATGGCACTGAGTTCCAAGGTCAACGCCGGTGCGCTGGCCAGCGATGCACCAGCCTTGATGATCGCGTGCGG
TCTGGCTTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472

Multiple sequence alignment