Detailed information NTG0396429

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: GH742_RS04845
• Genome accession: NZ_CP045732
• Coordinates: 1022800..1023210 (+)
• Length: 136 a.a.
• NCBI ID: WP_058525546.1
• Uniprot ID: A0A0W0TVG7
• Organism: Legionella sp. MW5194
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1017800..1028210

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GH742_RS04825 (GH742_04805)	-	1019747..1021153 (-)	1407	WP_203456334.1	amino acid permease	-
GH742_RS04830 (GH742_04810)	-	1021317..1021724 (+)	408	WP_203456335.1	hypothetical protein	-
GH742_RS04835 (GH742_04815)	-	1021726..1022169 (-)	444	WP_203456336.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
GH742_RS04840 (GH742_04820)	-	1022162..1022479 (-)	318	WP_203456337.1	BolA family transcriptional regulator	-
GH742_RS04845 (GH742_04825)	pilA2	1022800..1023210 (+)	411	WP_058525546.1	pilin	Machinery gene
GH742_RS04850 (GH742_04830)	pilA2	1023475..1023885 (+)	411	WP_203456338.1	pilin	Machinery gene
GH742_RS04855 (GH742_04835)	pilA2	1023997..1024407 (+)	411	WP_203456339.1	pilin	Machinery gene
GH742_RS04860 (GH742_04840)	-	1024436..1025671 (-)	1236	WP_203456340.1	6-phosphofructokinase	-

Sequence

Protein

• Length: 136 a.a.
• Molecular weight: 13782.05 Da
• Isoelectric Point: 9.0429

>NTDB_id=396429 GH742_RS04845 WP_058525546.1 1022800..1023210(+) (pilA2) [Legionella sp. MW5194]
MKQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLNLAASAKLAVSETAITNNALPATQAATGYVSPAATPNV
TSITIGAGGVVTITYTAAAGGGTIIMTPTLQANGDVTWVCTGGTLLAKYRPASCRP

Nucleotide

• Length: 411 bp

>NTDB_id=396429 GH742_RS04845 WP_058525546.1 1022800..1023210(+) (pilA2) [Legionella sp. MW5194]
ATGAAACAGAAGGGTTTTACACTGATTGAATTGATGATTGTGGTCGCGATTGTTGGTATTTTGGCAGCGATTGCAATTCC
CGCTTACCAGGACTATACGATTCGGGCGAGAGTAACGGAAGGTTTAAACCTTGCGGCTTCAGCCAAATTGGCAGTCTCTG
AAACAGCCATTACCAATAACGCCTTACCCGCTACGCAGGCCGCAACAGGTTATGTCAGCCCTGCTGCAACACCGAACGTT
ACGTCCATTACGATTGGCGCTGGCGGCGTGGTGACCATTACCTACACGGCGGCAGCCGGCGGTGGTACGATTATCATGAC
CCCGACCCTGCAGGCGAACGGCGATGTCACCTGGGTATGCACCGGCGGTACATTATTGGCTAAATACCGTCCAGCCAGTT
GCCGACCTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-135)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-25)

3D structure

• AlphaFold DB: A0A0W0TVG7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila strain ERS1305867	93.382	100	0.934
pilA2	Legionella pneumophila str. Paris	92.647	100	0.926
pilA	Ralstonia pseudosolanacearum GMI1000	50.932	100	0.603
comP	Acinetobacter baylyi ADP1	51.034	100	0.544
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	36.413	100	0.493
pilE	Neisseria gonorrhoeae strain FA1090	38.854	100	0.449
pilE	Neisseria gonorrhoeae MS11	38.854	100	0.449
pilA	Haemophilus influenzae 86-028NP	39.456	100	0.426
pilA/pilA1	Eikenella corrodens VA1	38.926	100	0.426
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.481	99.265	0.412
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.855	100	0.404
pilA	Haemophilus influenzae Rd KW20	39.568	100	0.404
pilA	Vibrio parahaemolyticus RIMD 2210633	40	95.588	0.382
pilA	Acinetobacter baumannii strain A118	37.956	100	0.382
pilA	Glaesserella parasuis strain SC1401	37.5	100	0.375
pilA	Vibrio cholerae C6706	34.459	100	0.375
pilA	Vibrio cholerae strain A1552	34.459	100	0.375
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	34.459	100	0.375