Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   GH742_RS04855 Genome accession   NZ_CP045732
Coordinates   1023997..1024407 (+) Length   136 a.a.
NCBI ID   WP_203456339.1    Uniprot ID   -
Organism   Legionella sp. MW5194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1018997..1029407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH742_RS04825 (GH742_04805) - 1019747..1021153 (-) 1407 WP_203456334.1 amino acid permease -
  GH742_RS04830 (GH742_04810) - 1021317..1021724 (+) 408 WP_203456335.1 hypothetical protein -
  GH742_RS04835 (GH742_04815) - 1021726..1022169 (-) 444 WP_203456336.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  GH742_RS04840 (GH742_04820) - 1022162..1022479 (-) 318 WP_203456337.1 BolA family transcriptional regulator -
  GH742_RS04845 (GH742_04825) pilA2 1022800..1023210 (+) 411 WP_058525546.1 pilin Machinery gene
  GH742_RS04850 (GH742_04830) pilA2 1023475..1023885 (+) 411 WP_203456338.1 pilin Machinery gene
  GH742_RS04855 (GH742_04835) pilA2 1023997..1024407 (+) 411 WP_203456339.1 pilin Machinery gene
  GH742_RS04860 (GH742_04840) - 1024436..1025671 (-) 1236 WP_203456340.1 6-phosphofructokinase -
  GH742_RS04865 (GH742_04845) letS 1025695..1028445 (-) 2751 WP_203456341.1 two-component system sensor histidine kinase LetS Regulator

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13776.04 Da        Isoelectric Point: 6.1474

>NTDB_id=396431 GH742_RS04855 WP_203456339.1 1023997..1024407(+) (pilA2) [Legionella sp. MW5194]
MNGKGFTLIELMIVVAIVGILAAVAIPVYQDYSIRARVAEGLTMAAPAKLAVSETTIDTSALPANQAATGYISPAASVNV
SSIVIAAAGVITITYTPVAGGGTIVLTPTLQANNDVAWDCRGGTLAAKYRPVSCVP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=396431 GH742_RS04855 WP_203456339.1 1023997..1024407(+) (pilA2) [Legionella sp. MW5194]
ATGAATGGAAAAGGCTTTACATTGATTGAACTTATGATTGTTGTGGCTATTGTGGGAATTCTCGCAGCAGTCGCCATTCC
CGTTTATCAGGATTATTCTATCCGGGCCCGCGTGGCCGAAGGCCTTACCATGGCGGCTCCCGCCAAATTGGCGGTCAGTG
AAACAACCATTGACACCAGTGCTTTACCCGCTAACCAGGCAGCCACGGGTTATATTAGTCCCGCCGCCTCGGTTAACGTG
TCTTCGATTGTTATTGCCGCGGCCGGTGTAATCACCATTACGTATACCCCAGTGGCTGGGGGAGGGACCATTGTGTTAAC
ACCAACTCTGCAAGCGAATAATGACGTGGCCTGGGACTGTCGCGGCGGCACGCTGGCGGCTAAATACCGCCCGGTAAGTT
GCGTGCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-25)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

76.471

100

0.765
  pilA2 Legionella pneumophila strain ERS1305867

75.735

100

0.757
  pilA Ralstonia pseudosolanacearum GMI1000

43.902

100

0.529
  comP Acinetobacter baylyi ADP1

46.853

100

0.493
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

32.967

100

0.441
  pilA Vibrio campbellii strain DS40M4

41.727

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.791

98.529

0.412
  pilE Neisseria gonorrhoeae MS11

35.948

100

0.404
  pilA/pilA1 Eikenella corrodens VA1

34.437

100

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.957

100

0.375
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.17

100

0.375
  pilA Vibrio cholerae strain A1552

36.17

100

0.375
  pilA Vibrio cholerae C6706

36.17

100

0.375
  pilA Haemophilus influenzae Rd KW20

38.462

95.588

0.368

Multiple sequence alignment