Detailed information NTG0347518

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: EXU30_RS20790
• Genome accession: NZ_CP036200
• Coordinates: 2673691..2674128 (-)
• Length: 145 a.a.
• NCBI ID: WP_130600176.1
• Uniprot ID: A0A411PI16
• Organism: Shewanella maritima strain D4-2
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2668691..2679128

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXU30_RS11475 (EXU30_11475)	pilB	2669836..2671545 (-)	1710	WP_130600172.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EXU30_RS11480 (EXU30_11480)	-	2671655..2672992 (-)	1338	WP_242620407.1	sulfatase-like hydrolase/transferase	-
EXU30_RS20610	-	2672893..2673621 (-)	729	WP_242620197.1	hypothetical protein	-
EXU30_RS20790 (EXU30_11485)	pilA	2673691..2674128 (-)	438	WP_130600176.1	pilin	Machinery gene
EXU30_RS11490 (EXU30_11490)	nadC	2675205..2676083 (-)	879	WP_130600178.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EXU30_RS11495 (EXU30_11495)	ampD	2676378..2676944 (+)	567	WP_130600180.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
EXU30_RS11500 (EXU30_11500)	ampE	2677098..2677949 (+)	852	WP_130600182.1	beta-lactamase regulator AmpE	-
EXU30_RS11505 (EXU30_11505)	pdhR	2678259..2679011 (+)	753	WP_130600184.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 14659.72 Da
• Isoelectric Point: 9.0026

>NTDB_id=347518 EXU30_RS20790 WP_130600176.1 2673691..2674128(-) (pilA) [Shewanella maritima strain D4-2]
MKGINQVKNAKGFTLIELMIVVAIIGILAAIALPAYQDFTKRGHVSEGLSLAAGAKAAVTEYYSSQGSAPTTNALAGLAA
ASSITGNSVKSVAVGTGGGITVTYNAKVTDDATLILAPTFTSSGNVTWACTGGSLDDKYKPSNCR

Nucleotide

• Length: 438 bp

>NTDB_id=347518 EXU30_RS20790 WP_130600176.1 2673691..2674128(-) (pilA) [Shewanella maritima strain D4-2]
ATGAAAGGTATCAACCAAGTTAAAAACGCTAAGGGTTTTACTCTTATTGAATTAATGATCGTAGTTGCGATCATCGGTAT
TCTTGCTGCAATCGCACTGCCTGCGTATCAAGACTTCACTAAACGTGGTCATGTTTCTGAAGGTCTTAGTCTTGCTGCAG
GAGCAAAAGCTGCAGTGACTGAATATTACTCTTCACAAGGCTCTGCACCAACAACCAATGCTCTTGCTGGGCTAGCAGCA
GCATCTTCAATTACTGGTAACTCAGTTAAGTCTGTAGCGGTTGGTACAGGCGGTGGCATTACAGTAACATACAACGCCAA
AGTAACAGATGACGCAACTCTTATTCTGGCTCCAACTTTTACCTCTTCAGGTAACGTTACATGGGCATGTACTGGTGGTT
CTCTTGACGACAAATATAAACCATCTAACTGCCGTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (42-145)

• Prokaryotic N-terminal methylation motif (N_methyl) (8-33)

3D structure

• AlphaFold DB: A0A411PI16

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Ralstonia pseudosolanacearum GMI1000	42.857	100	0.517
pilA2	Legionella pneumophila strain ERS1305867	53.333	93.103	0.497
pilA2	Legionella pneumophila str. Paris	52.593	93.103	0.49
pilE	Neisseria gonorrhoeae strain FA1090	44.516	100	0.476
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.158	100	0.469
comP	Acinetobacter baylyi ADP1	45.833	99.31	0.455
pilE	Neisseria gonorrhoeae MS11	42.208	100	0.448
pilA/pilA1	Eikenella corrodens VA1	40	100	0.414
pilA	Haemophilus influenzae 86-028NP	40.972	99.31	0.407
pilA/pilAII	Pseudomonas stutzeri DSM 10701	40	96.552	0.386
pilA	Haemophilus influenzae Rd KW20	41.791	92.414	0.386
pilA	Vibrio campbellii strain DS40M4	40.441	93.793	0.379
pilA	Vibrio parahaemolyticus RIMD 2210633	40.299	92.414	0.372
pilA	Acinetobacter baumannii strain A118	38.849	95.862	0.372
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.13	95.172	0.372
pilA	Vibrio cholerae C6706	35.811	100	0.366
pilA	Vibrio cholerae strain A1552	35.811	100	0.366
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	35.811	100	0.366
pilA	Glaesserella parasuis strain SC1401	35.57	100	0.366