Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EXU30_RS11475 Genome accession   NZ_CP036200
Coordinates   2669836..2671545 (-) Length   569 a.a.
NCBI ID   WP_130600172.1    Uniprot ID   A0A411PI25
Organism   Shewanella maritima strain D4-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2664836..2676545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXU30_RS11445 (EXU30_11445) mutT 2665436..2665843 (-) 408 WP_130600160.1 8-oxo-dGTP diphosphatase MutT -
  EXU30_RS11450 (EXU30_11450) yacG 2665850..2666077 (-) 228 WP_130600162.1 DNA gyrase inhibitor YacG -
  EXU30_RS11455 (EXU30_11455) zapD 2666119..2666853 (-) 735 WP_130600164.1 cell division protein ZapD -
  EXU30_RS11460 (EXU30_11460) coaE 2666883..2667507 (-) 625 Protein_2253 dephospho-CoA kinase -
  EXU30_RS11465 (EXU30_11465) pilD 2667527..2668468 (-) 942 WP_130600168.1 A24 family peptidase Machinery gene
  EXU30_RS11470 (EXU30_11470) pilC 2668515..2669768 (-) 1254 WP_130600170.1 type II secretion system F family protein Machinery gene
  EXU30_RS11475 (EXU30_11475) pilB 2669836..2671545 (-) 1710 WP_130600172.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EXU30_RS11480 (EXU30_11480) - 2671655..2672992 (-) 1338 WP_242620407.1 sulfatase-like hydrolase/transferase -
  EXU30_RS20610 - 2672893..2673621 (-) 729 WP_242620197.1 hypothetical protein -
  EXU30_RS20790 (EXU30_11485) pilA 2673691..2674128 (-) 438 WP_130600176.1 pilin Machinery gene
  EXU30_RS11490 (EXU30_11490) nadC 2675205..2676083 (-) 879 WP_130600178.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62831.87 Da        Isoelectric Point: 4.8949

>NTDB_id=347510 EXU30_RS11475 WP_130600172.1 2669836..2671545(-) (pilB) [Shewanella maritima strain D4-2]
MPSTAINLGLSNLFLRKGLLTEETLSSAISESRKTKRTLVSTLVATKKLSAREIAELCYEEYGTPLLDLSEYDVKNIPED
LLNKKLIDKHKCLPLFKRGNRLYIASSDPTNISALEDFQFSLGLHAEAILVEDDKLSQAVEKVLEDDLSSLDLSGVDEEA
LAGMEVAETERNDEPEGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLAGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDEQRLLYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLVNMGVPGYNIASSVNLIIAQRLARRLCPECKEPEDVPQAELVKLGFT
EQDIATGFTVYKPVGCEFCSGGYKGRVGVYEVMKMSDEIARTIMEGGNSLQIANQAKEQGMNDLRQSGLLKVVQGVTSIA
EINRVTSFN

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=347510 EXU30_RS11475 WP_130600172.1 2669836..2671545(-) (pilB) [Shewanella maritima strain D4-2]
ATGCCATCGACAGCTATAAACTTAGGCTTATCAAATCTTTTCCTGAGAAAAGGGCTTTTGACTGAAGAAACGCTTTCAAG
CGCAATATCAGAATCAAGAAAAACAAAGCGTACTCTTGTCTCGACTTTAGTTGCGACAAAGAAGCTGTCTGCTAGAGAAA
TTGCTGAGCTTTGTTACGAAGAGTATGGTACTCCATTACTAGACTTAAGCGAGTATGATGTTAAAAACATCCCTGAAGAC
TTACTCAACAAAAAGCTAATCGATAAGCATAAGTGTCTTCCATTGTTCAAAAGGGGAAATCGCTTATACATCGCAAGCTC
AGATCCAACCAATATTTCCGCATTAGAAGACTTTCAATTTAGCTTGGGTCTTCACGCTGAAGCGATTCTAGTTGAAGATG
ACAAACTGTCCCAAGCAGTAGAAAAAGTGCTTGAAGATGATCTATCTAGCCTAGATCTCTCTGGAGTCGACGAGGAAGCC
CTTGCTGGGATGGAGGTGGCAGAAACCGAACGTAATGATGAGCCCGAAGGCGACAGCAGTGACGATGCCCCAATTGTTAT
TTACATCAACAAGATTCTAACCGACGCCATCCGTAAAGGCGCTTCTGACTTGCACTTCGAGCCATATGAAAAACGCTACC
GCATTCGTTTTCGCATCGATGGGATTTTACACGAGGTTTCAGAACCACCTGTAAACCTGGCAGGTCGAATCTCAGCACGA
TTAAAGGTGATGTCAAAGCTGGATATTGCTGAGCGCCGCGTGCCTCAAGATGGCCGCATTAAGATGAAGCTGTCACGTAC
TAAATCAATTGATTTTCGTGTCAGTACTCTACCTACAATCTGGGGCGAGAAGATTGTAATGCGTATTCTGGATTCATCTT
CCGCTCAGCTTGGTATCGAAAAACTCGGTTATGAAGATGAGCAGCGCCTGCTTTATGAAGAAATGCTAGCCAAACCCCAA
GGCATGATATTAGTGACCGGCCCAACTGGCTCCGGTAAAACTGTATCCCTTTATACTGGCCTTAATATCCTTAATACGGA
AGAGCGTAATATTTCAACTGCTGAAGATCCGGTTGAGATTAACTTAGAAGGTGTTAACCAAGTTCATATTAACTTGAAAG
CAGGTTTAACCTTCGCTTCAGCTCTTCGCTCGTTCTTACGTCAAGATCCAGACGTGGTAATGGTCGGTGAGATCCGTGAC
TTAGAAACCGCTGAGATAGCCATTAAAGCGGCACAAACCGGTCACTTGGTTTTATCAACTCTCCATACAAACTCAGCAGC
AGAGACCTTAACCCGTTTGGTTAACATGGGCGTACCTGGCTACAACATTGCCAGCTCAGTCAACCTAATTATTGCACAGC
GTCTTGCAAGGCGTTTATGCCCTGAGTGTAAAGAGCCTGAAGACGTGCCTCAAGCAGAGCTAGTAAAGCTTGGTTTTACT
GAACAAGATATCGCAACAGGGTTCACCGTATACAAGCCTGTTGGTTGTGAATTCTGTTCTGGAGGTTACAAAGGCCGTGT
AGGTGTCTATGAGGTAATGAAAATGAGTGATGAAATCGCCCGAACTATTATGGAAGGCGGTAATTCACTGCAAATAGCTA
ATCAAGCAAAAGAACAAGGCATGAATGACTTACGTCAATCAGGGCTGCTAAAGGTCGTTCAAGGTGTGACGAGTATTGCT
GAGATTAACCGCGTAACTAGTTTCAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411PI25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.363

98.77

0.576
  pilB Acinetobacter baumannii D1279779

60.073

95.958

0.576
  pilB Legionella pneumophila strain ERS1305867

58.769

94.2

0.554
  pilB Vibrio cholerae strain A1552

52.028

99.649

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

52.043

98.946

0.515
  pilB Vibrio campbellii strain DS40M4

50.266

98.946

0.497
  pilF Neisseria gonorrhoeae MS11

48.319

99.297

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

37.013

100

0.401
  pilF Thermus thermophilus HB27

38.79

98.77

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.138

97.891

0.383

Multiple sequence alignment