Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CDN75_RS10580 Genome accession   NZ_CP025520
Coordinates   2021914..2022573 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain DH5alpha     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2016914..2027573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDN75_RS10545 ygiS 2018197..2019804 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  CDN75_RS10555 mqsA 2019937..2020332 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  CDN75_RS10560 mqsR 2020334..2020630 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  CDN75_RS10565 ygiV 2020835..2021317 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  CDN75_RS10570 ygiW 2021370..2021762 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CDN75_RS10580 ciaR 2021914..2022573 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  CDN75_RS10585 qseC 2022570..2023919 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  CDN75_RS10590 ygiZ 2023965..2024297 (-) 333 WP_000917684.1 DUF2645 family protein -
  CDN75_RS10600 mdaB 2024616..2025197 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CDN75_RS10605 ygiN 2025228..2025542 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CDN75_RS10610 parE 2025590..2027482 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=219260 CDN75_RS10580 WP_001221495.1 2021914..2022573(+) (ciaR) [Escherichia coli strain DH5alpha]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=219260 CDN75_RS10580 WP_001221495.1 2021914..2022573(+) (ciaR) [Escherichia coli strain DH5alpha]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361

Multiple sequence alignment