Detailed information NTG1147443

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: CKV84_RS09385
• Genome accession: NZ_LT906452
• Coordinates: 2046540..2046950 (-)
• Length: 136 a.a.
• NCBI ID: WP_014844212.1
• Uniprot ID: A0A378K550
• Organism: Legionella pneumophila strain NCTC11985
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2041540..2051950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV84_RS09370 (SAMEA4040028_01860)	letS	2041859..2044591 (+)	2733	WP_027220386.1	two-component system sensor histidine kinase LetS	Regulator
CKV84_RS09375 (SAMEA4040028_01861)	-	2044689..2045933 (+)	1245	WP_011214166.1	6-phosphofructokinase	-
CKV84_RS09380 (SAMEA4040028_01862)	pilA2	2045930..2046343 (-)	414	WP_013101573.1	pilin	Machinery gene
CKV84_RS09385 (SAMEA4040028_01863)	pilA2	2046540..2046950 (-)	411	WP_014844212.1	pilin	Machinery gene
CKV84_RS09390 (SAMEA4040028_01864)	-	2047253..2047570 (+)	318	WP_010947633.1	BolA family protein	-
CKV84_RS09395 (SAMEA4040028_01865)	-	2047645..2049066 (+)	1422	WP_027220385.1	APC family permease	-
CKV84_RS09400 (SAMEA4040028_01866)	-	2049166..2050596 (-)	1431	WP_027220384.1	cellulase family glycosylhydrolase	-
CKV84_RS09405 (SAMEA4040028_01867)	kdsB	2050843..2051595 (-)	753	WP_027220383.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
CKV84_RS09410 (SAMEA4040028_01868)	-	2051592..2051771 (-)	180	WP_010947637.1	Trm112 family protein	-

Sequence

Protein

• Length: 136 a.a.
• Molecular weight: 13925.15 Da
• Isoelectric Point: 8.4715

>NTDB_id=1147443 CKV84_RS09385 WP_014844212.1 2046540..2046950(-) (pilA2) [Legionella pneumophila strain NCTC11985]
MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTLADSAKLAVSETAITNNALPATQAATGYVSPAATPNV
QSIAIGANGVITITYTAAAGGGTIIMTPTLQANGDVTWTCTGGTLLAKYRPASCRP

Nucleotide

• Length: 411 bp

>NTDB_id=1147443 CKV84_RS09385 WP_014844212.1 2046540..2046950(-) (pilA2) [Legionella pneumophila strain NCTC11985]
ATGAGACAAAAGGGTTTTACTTTAATTGAGTTGATGATAGTCGTAGCGATTATCGGTATTTTGGCAGCAATCGCTATACC
AGCCTATCAAGATTACACAATACGAGCTCGTGTTACTGAAGGTCTGACCTTGGCAGATTCTGCAAAACTTGCTGTATCTG
AAACGGCTATTACAAACAACGCGTTACCAGCCACTCAGGCTGCAACGGGCTATGTGTCTCCTGCTGCTACGCCCAATGTA
CAATCAATTGCTATTGGTGCTAACGGTGTGATTACCATAACGTATACTGCTGCAGCAGGTGGTGGTACCATTATTATGAC
CCCAACTTTACAAGCAAACGGTGACGTAACCTGGACTTGTACTGGTGGTACATTATTGGCCAAATACCGACCTGCCAGTT
GCAGACCATAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-135)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-25)

3D structure

• AlphaFold DB: A0A378K550

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila strain ERS1305867	100	100	1
pilA2	Legionella pneumophila str. Paris	98.529	100	0.985
pilA	Ralstonia pseudosolanacearum GMI1000	49.068	100	0.581
comP	Acinetobacter baylyi ADP1	53.103	100	0.566
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.566	100	0.522
pilA/pilA1	Eikenella corrodens VA1	39.873	100	0.463
pilE	Neisseria gonorrhoeae strain FA1090	38.462	100	0.441
pilE	Neisseria gonorrhoeae MS11	38.217	100	0.441
pilA	Haemophilus influenzae 86-028NP	40.816	100	0.441
pilA	Pseudomonas aeruginosa PAK	39.865	100	0.434
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.963	99.265	0.426
pilA	Acinetobacter baumannii strain A118	39.041	100	0.419
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.855	100	0.404
pilA	Haemophilus influenzae Rd KW20	43.307	93.382	0.404
pilA	Vibrio cholerae C6706	37.762	100	0.397
pilA	Vibrio cholerae strain A1552	37.762	100	0.397
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.762	100	0.397
pilA	Glaesserella parasuis strain SC1401	38.235	100	0.382
pilA	Vibrio parahaemolyticus RIMD 2210633	38.462	95.588	0.368