Detailed information NTG1147231

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: CKW04_RS06635
• Genome accession: NZ_LT906442
• Coordinates: 1411335..1411748 (+)
• Length: 137 a.a.
• NCBI ID: WP_058479452.1
• Uniprot ID: A0A0W1AMK4
• Organism: Legionella waltersii strain NCTC13017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1406335..1416748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKW04_RS06605 (SAMEA4504053_01346)	-	1406558..1407166 (+)	609	WP_231951028.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
CKW04_RS06610 (SAMEA4504053_01347)	tsaB	1407233..1407904 (+)	672	WP_231951048.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
CKW04_RS06615 (SAMEA4504053_01348)	-	1407897..1408076 (+)	180	WP_058479448.1	Trm112 family protein	-
CKW04_RS06620 (SAMEA4504053_01349)	kdsB	1408073..1408810 (+)	738	WP_058479449.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
CKW04_RS06625 (SAMEA4504053_01350)	-	1409243..1410667 (-)	1425	WP_058479450.1	APC family permease	-
CKW04_RS06630 (SAMEA4504053_01351)	-	1410733..1411050 (-)	318	WP_058479451.1	BolA family transcriptional regulator	-
CKW04_RS06635 (SAMEA4504053_01352)	pilA2	1411335..1411748 (+)	414	WP_058479452.1	pilin	Machinery gene
CKW04_RS06640 (SAMEA4504053_01353)	pilA2	1412025..1412438 (+)	414	WP_058479453.1	pilin	Machinery gene
CKW04_RS06645 (SAMEA4504053_01354)	-	1412435..1413673 (-)	1239	WP_058479483.1	6-phosphofructokinase	-
CKW04_RS06650 (SAMEA4504053_01355)	letS	1413687..1416419 (-)	2733	WP_083500025.1	two-component system sensor histidine kinase LetS	Regulator

Sequence

Protein

• Length: 137 a.a.
• Molecular weight: 14045.08 Da
• Isoelectric Point: 4.9002

>NTDB_id=1147231 CKW04_RS06635 WP_058479452.1 1411335..1411748(+) (pilA2) [Legionella waltersii strain NCTC13017]
MKQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLQLADSAKLAVSETAITNNALPANQAATGYVSPAATANV
QSIAVDGTGNGQITITYTAAAGGGTIVMAPTLQANGDVTWDCTGGTLLDKYRPASCR

Nucleotide

• Length: 414 bp

>NTDB_id=1147231 CKW04_RS06635 WP_058479452.1 1411335..1411748(+) (pilA2) [Legionella waltersii strain NCTC13017]
ATGAAACAGAAAGGTTTTACATTAATTGAATTGATGATCGTGGTAGCTATTATCGGTATTTTAGCTGCAATAGCTATACC
AGCTTATCAAGACTATACCATTAGAGCTCGTGTAACAGAGGGTTTACAATTAGCTGACTCTGCGAAACTAGCTGTATCTG
AAACAGCCATCACCAACAACGCATTACCTGCAAACCAAGCTGCTACTGGATACGTGTCTCCAGCTGCTACAGCTAACGTG
CAAAGTATCGCTGTTGACGGAACTGGTAACGGTCAAATAACCATTACTTATACTGCTGCTGCAGGCGGGGGTACCATTGT
TATGGCCCCAACTCTCCAAGCTAACGGTGACGTCACTTGGGATTGTACTGGCGGTACTTTGTTGGATAAATACAGACCAG
CTAGTTGCAGATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-25)

• Pilin (bacterial filament) (Pilin) (35-137)

3D structure

• AlphaFold DB: A0A0W1AMK4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	89.781	100	0.898
pilA2	Legionella pneumophila strain ERS1305867	89.781	100	0.898
pilA	Ralstonia pseudosolanacearum GMI1000	50.932	100	0.599
comP	Acinetobacter baylyi ADP1	51.034	100	0.54
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	36.957	100	0.496
pilA/pilA1	Eikenella corrodens VA1	38.71	100	0.438
pilE	Neisseria gonorrhoeae strain FA1090	37.821	100	0.431
pilE	Neisseria gonorrhoeae MS11	37.179	100	0.423
pilA	Vibrio cholerae strain A1552	38.514	100	0.416
pilA	Vibrio cholerae C6706	38.514	100	0.416
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	38.514	100	0.416
pilA	Haemophilus influenzae Rd KW20	41.606	100	0.416
pilA/pilAII	Pseudomonas stutzeri DSM 10701	41.304	100	0.416
pilA	Haemophilus influenzae 86-028NP	43.077	94.891	0.409
pilA	Pseudomonas aeruginosa PAK	36.842	100	0.409
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.741	98.54	0.401
pilA	Vibrio parahaemolyticus RIMD 2210633	40.602	97.08	0.394
pilA	Acinetobacter baumannii strain A118	38.849	100	0.394
comF	Acinetobacter baylyi ADP1	37.879	96.35	0.365