Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CKW04_RS06640 Genome accession   NZ_LT906442
Coordinates   1412025..1412438 (+) Length   137 a.a.
NCBI ID   WP_058479453.1    Uniprot ID   A0A0W1AMU8
Organism   Legionella waltersii strain NCTC13017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1407025..1417438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW04_RS06610 (SAMEA4504053_01347) tsaB 1407233..1407904 (+) 672 WP_231951048.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  CKW04_RS06615 (SAMEA4504053_01348) - 1407897..1408076 (+) 180 WP_058479448.1 Trm112 family protein -
  CKW04_RS06620 (SAMEA4504053_01349) kdsB 1408073..1408810 (+) 738 WP_058479449.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  CKW04_RS06625 (SAMEA4504053_01350) - 1409243..1410667 (-) 1425 WP_058479450.1 APC family permease -
  CKW04_RS06630 (SAMEA4504053_01351) - 1410733..1411050 (-) 318 WP_058479451.1 BolA family transcriptional regulator -
  CKW04_RS06635 (SAMEA4504053_01352) pilA2 1411335..1411748 (+) 414 WP_058479452.1 pilin Machinery gene
  CKW04_RS06640 (SAMEA4504053_01353) pilA2 1412025..1412438 (+) 414 WP_058479453.1 pilin Machinery gene
  CKW04_RS06645 (SAMEA4504053_01354) - 1412435..1413673 (-) 1239 WP_058479483.1 6-phosphofructokinase -
  CKW04_RS06650 (SAMEA4504053_01355) letS 1413687..1416419 (-) 2733 WP_083500025.1 two-component system sensor histidine kinase LetS Regulator

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14244.55 Da        Isoelectric Point: 6.1605

>NTDB_id=1147232 CKW04_RS06640 WP_058479453.1 1412025..1412438(+) (pilA2) [Legionella waltersii strain NCTC13017]
MQVRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTMAASAKLAVAETSLFEGALPANQAQTGYVTPAPTVNV
ASITIGNQGVITINYTLAAGNGSITLVPVIDAAGQIYTWDCTGGTLISKYRPASCRP

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1147232 CKW04_RS06640 WP_058479453.1 1412025..1412438(+) (pilA2) [Legionella waltersii strain NCTC13017]
ATGCAAGTCCGCGGATTTACTCTCATTGAGCTAATGATTGTAGTAGCAATCATTGGTATCTTGGCAGCGATAGCAATTCC
TGCTTATCAAGACTACACTATCCGGGCGAGAGTAACAGAGGGTCTTACAATGGCTGCTTCAGCTAAATTAGCAGTTGCAG
AAACCAGTTTATTCGAAGGGGCTCTTCCTGCTAATCAAGCTCAGACAGGCTATGTCACTCCTGCTCCAACTGTTAATGTT
GCTTCAATTACAATTGGAAACCAAGGAGTGATTACCATCAATTATACACTGGCCGCTGGAAATGGATCTATTACATTGGT
TCCTGTGATTGATGCTGCTGGGCAGATTTACACTTGGGACTGTACAGGAGGCACTTTGATAAGTAAGTACCGTCCTGCAA
GTTGTAGGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W1AMU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

73.723

100

0.737
  pilA2 Legionella pneumophila strain ERS1305867

72.993

100

0.73
  pilA Ralstonia pseudosolanacearum GMI1000

47.5

100

0.555
  comP Acinetobacter baylyi ADP1

50

100

0.533
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.69

100

0.46
  pilA/pilA1 Eikenella corrodens VA1

40.789

100

0.453
  pilA Vibrio cholerae strain A1552

38

100

0.416
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38

100

0.416
  pilA Vibrio cholerae C6706

38

100

0.416
  pilE Neisseria gonorrhoeae MS11

36.774

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.716

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.732

100

0.401
  comF Acinetobacter baylyi ADP1

44.628

88.321

0.394

Multiple sequence alignment