Detailed information NTG1122509

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: EL201_RS09825
• Genome accession: NZ_LR134380
• Coordinates: 2160546..2160956 (-)
• Length: 136 a.a.
• NCBI ID: WP_011214168.1
• Uniprot ID: A0AAX2J0V3
• Organism: Legionella pneumophila subsp. pascullei strain NCTC12273
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2155546..2165956

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL201_RS09810 (NCTC12273_01970)	letS	2155866..2158598 (+)	2733	WP_027222091.1	two-component system sensor histidine kinase LetS	Regulator
EL201_RS09815 (NCTC12273_01971)	-	2158696..2159940 (+)	1245	WP_027222092.1	6-phosphofructokinase	-
EL201_RS09820 (NCTC12273_01972)	pilA2	2159937..2160350 (-)	414	WP_027222093.1	pilin	Machinery gene
EL201_RS09825 (NCTC12273_01973)	pilA2	2160546..2160956 (-)	411	WP_011214168.1	pilin	Machinery gene
EL201_RS09830 (NCTC12273_01974)	-	2161258..2161575 (+)	318	WP_027222094.1	BolA family protein	-
EL201_RS09835 (NCTC12273_01975)	-	2161650..2163071 (+)	1422	WP_027222095.1	APC family permease	-
EL201_RS09840 (NCTC12273_01976)	-	2163203..2164633 (-)	1431	WP_027222096.1	cellulase family glycosylhydrolase	-
EL201_RS09845 (NCTC12273_01977)	kdsB	2164881..2165633 (-)	753	WP_027222097.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
EL201_RS09850 (NCTC12273_01978)	-	2165630..2165809 (-)	180	WP_010947637.1	Trm112 family protein	-

Sequence

Protein

• Length: 136 a.a.
• Molecular weight: 13854.07 Da
• Isoelectric Point: 8.4715

>NTDB_id=1122509 EL201_RS09825 WP_011214168.1 2160546..2160956(-) (pilA2) [Legionella pneumophila subsp. pascullei strain NCTC12273]
MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTLADSAKLAVSETAITNNALPATQAATGYVSPAATPNV
ASIAIGANGVITITYTAAAGGGTIVMTPTLQANGDVTWTCTGGTLLAKYRPASCRP

Nucleotide

• Length: 411 bp

>NTDB_id=1122509 EL201_RS09825 WP_011214168.1 2160546..2160956(-) (pilA2) [Legionella pneumophila subsp. pascullei strain NCTC12273]
ATGAGACAAAAGGGTTTTACATTAATTGAATTGATGATAGTCGTAGCGATTATCGGCATTTTGGCAGCAATCGCTATACC
AGCCTATCAAGATTACACAATACGAGCTCGTGTTACTGAAGGTCTGACTTTAGCAGATTCTGCAAAACTTGCCGTATCTG
AAACGGCTATCACTAACAATGCCTTACCAGCTACTCAAGCGGCAACGGGGTACGTGTCTCCTGCTGCTACGCCCAATGTA
GCGTCAATTGCTATTGGTGCTAACGGTGTTATTACCATAACGTATACTGCCGCAGCTGGTGGTGGTACCATTGTTATGAC
CCCAACCTTACAAGCTAATGGTGATGTAACCTGGACTTGTACGGGTGGTACTTTATTGGCTAAATATCGACCTGCCAGTT
GCAGACCATAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-135)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-25)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	100	100	1
pilA2	Legionella pneumophila strain ERS1305867	98.529	100	0.985
pilA	Ralstonia pseudosolanacearum GMI1000	49.689	100	0.588
comP	Acinetobacter baylyi ADP1	53.793	100	0.574
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.566	100	0.522
pilA/pilA1	Eikenella corrodens VA1	39.873	100	0.463
pilA	Haemophilus influenzae 86-028NP	40.816	100	0.441
pilE	Neisseria gonorrhoeae strain FA1090	36.943	100	0.426
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.963	99.265	0.426
pilA	Acinetobacter baumannii strain A118	39.726	100	0.426
pilA	Pseudomonas aeruginosa PAK	39.189	100	0.426
pilE	Neisseria gonorrhoeae MS11	37.179	100	0.426
pilA	Haemophilus influenzae Rd KW20	44.094	93.382	0.412
pilA	Vibrio cholerae C6706	38.462	100	0.404
pilA	Vibrio cholerae strain A1552	38.462	100	0.404
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	38.462	100	0.404
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.855	100	0.404
pilA	Glaesserella parasuis strain SC1401	37.5	100	0.375
pilA	Vibrio parahaemolyticus RIMD 2210633	38.462	95.588	0.368