Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AADV58_RS03080 Genome accession   NZ_CP151406
Coordinates   629597..630640 (+) Length   347 a.a.
NCBI ID   WP_028993621.1    Uniprot ID   -
Organism   Azonexus hydrophilus strain NCP973     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624597..635640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADV58_RS03050 (AADV58_03050) - 625528..626772 (+) 1245 WP_341744049.1 dihydroorotase -
  AADV58_RS03055 (AADV58_03055) comE 626914..627189 (+) 276 WP_028993626.1 helix-hairpin-helix domain-containing protein Machinery gene
  AADV58_RS03060 (AADV58_03060) - 627214..627504 (-) 291 WP_028993625.1 DUF167 domain-containing protein -
  AADV58_RS03065 (AADV58_03065) - 627504..628037 (-) 534 WP_298394755.1 YggT family protein -
  AADV58_RS03070 (AADV58_03070) proC 628037..628852 (-) 816 WP_028993623.1 pyrroline-5-carboxylate reductase -
  AADV58_RS03075 (AADV58_03075) - 628849..629541 (-) 693 WP_028993622.1 YggS family pyridoxal phosphate-dependent enzyme -
  AADV58_RS03080 (AADV58_03080) pilT 629597..630640 (+) 1044 WP_028993621.1 type IV pilus twitching motility protein PilT Machinery gene
  AADV58_RS03085 (AADV58_03085) pilU 630668..631804 (+) 1137 WP_028993620.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AADV58_RS03090 (AADV58_03090) - 631822..632511 (+) 690 WP_341744050.1 DUF4197 domain-containing protein -
  AADV58_RS03095 (AADV58_03095) - 632572..632889 (+) 318 WP_281984693.1 hypothetical protein -
  AADV58_RS03100 (AADV58_03100) - 632994..634982 (+) 1989 WP_341744051.1 UvrD-helicase domain-containing protein -
  AADV58_RS03105 (AADV58_03105) - 635032..635481 (-) 450 WP_341744052.1 c-type cytochrome -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38466.20 Da        Isoelectric Point: 6.6607

>NTDB_id=976439 AADV58_RS03080 WP_028993621.1 629597..630640(+) (pilT) [Azonexus hydrophilus strain NCP973]
MDITELLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEHKDVHGMVYDIMNDSQRKIFEETLECDFSFEIPNLAR
FRVNAFNQQRGAAAVFRTIPTKVLTLEELNCPKIFKDISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENDYAHILTIED
PIEFVHESKKCLINQREVGPQTLSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQAQGMQTLDQC
LIDLVRRNVVASGEARLRAANKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=976439 AADV58_RS03080 WP_028993621.1 629597..630640(+) (pilT) [Azonexus hydrophilus strain NCP973]
ATGGACATCACCGAACTGCTGGCCTTCAGCGTCAAGAACAAGGCATCCGACCTTCACCTGTCGTCCGGCTTGCCGCCGAT
GATTCGCGTCCATGGTGACGTGCGCCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACGGCATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAGATTTTCGAGGAAACGCTGGAATGCGATTTCTCCTTCGAGATTCCCAACCTGGCCCGC
TTCCGGGTTAACGCCTTCAACCAGCAACGCGGTGCCGCCGCCGTCTTCCGGACGATTCCGACCAAGGTGCTGACGCTGGA
AGAGCTGAACTGCCCGAAGATATTCAAGGATATTTCCGAGTATCCGCGCGGCATCGTGCTGGTCACCGGCCCGACCGGTT
CCGGCAAGTCGACGACGCTGGCGGCGATGGTCAACCACGTCAATGAAAACGACTACGCGCACATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCATGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGTCCGCAGACCCTGTCTTTCTCGAA
CGCCTTGCGTTCGGCGCTGCGTGAAGACCCGGATGTGATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCTTGG
CGCTGACTGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCTTGCATACCTCGTCGGCCGCCAAGACCATCGACCGGGTG
GTCGACGTCTTCCCCGCTGACGAAAAGGAAATGGTCCGCGCCATGCTGTCCGAATCGCTGCGTGCCGTCATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGTCAAGGCCGCGTAGCAGCCCACGAGATCATGATCGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCACAGATGTATTCGGCGATCCAGACCGGCCAGGCCCAGGGTATGCAGACGCTCGACCAGTGT
CTGATCGATCTGGTCCGCCGCAACGTCGTGGCCAGCGGCGAAGCCCGCCTGCGCGCCGCCAACAAGGACGCCTTCCCGGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.261

99.424

0.778
  pilT Acinetobacter baumannii D1279779

78.261

99.424

0.778
  pilT Acinetobacter baumannii strain A118

78.261

99.424

0.778
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Neisseria meningitidis 8013

72.754

99.424

0.723
  pilT Neisseria gonorrhoeae MS11

72.464

99.424

0.72
  pilT Legionella pneumophila strain Lp02

71.884

99.424

0.715
  pilT Legionella pneumophila strain ERS1305867

71.884

99.424

0.715
  pilT Vibrio cholerae strain A1552

70.238

96.83

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.238

96.83

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.593

97.695

0.406
  pilU Acinetobacter baylyi ADP1

41.369

96.83

0.401
  pilU Vibrio cholerae strain A1552

40.299

96.542

0.389

Multiple sequence alignment