Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AADV58_RS03085 Genome accession   NZ_CP151406
Coordinates   630668..631804 (+) Length   378 a.a.
NCBI ID   WP_028993620.1    Uniprot ID   -
Organism   Azonexus hydrophilus strain NCP973     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 625668..636804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADV58_RS03055 (AADV58_03055) comE 626914..627189 (+) 276 WP_028993626.1 helix-hairpin-helix domain-containing protein Machinery gene
  AADV58_RS03060 (AADV58_03060) - 627214..627504 (-) 291 WP_028993625.1 DUF167 domain-containing protein -
  AADV58_RS03065 (AADV58_03065) - 627504..628037 (-) 534 WP_298394755.1 YggT family protein -
  AADV58_RS03070 (AADV58_03070) proC 628037..628852 (-) 816 WP_028993623.1 pyrroline-5-carboxylate reductase -
  AADV58_RS03075 (AADV58_03075) - 628849..629541 (-) 693 WP_028993622.1 YggS family pyridoxal phosphate-dependent enzyme -
  AADV58_RS03080 (AADV58_03080) pilT 629597..630640 (+) 1044 WP_028993621.1 type IV pilus twitching motility protein PilT Machinery gene
  AADV58_RS03085 (AADV58_03085) pilU 630668..631804 (+) 1137 WP_028993620.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AADV58_RS03090 (AADV58_03090) - 631822..632511 (+) 690 WP_341744050.1 DUF4197 domain-containing protein -
  AADV58_RS03095 (AADV58_03095) - 632572..632889 (+) 318 WP_281984693.1 hypothetical protein -
  AADV58_RS03100 (AADV58_03100) - 632994..634982 (+) 1989 WP_341744051.1 UvrD-helicase domain-containing protein -
  AADV58_RS03105 (AADV58_03105) - 635032..635481 (-) 450 WP_341744052.1 c-type cytochrome -
  AADV58_RS03110 (AADV58_03110) - 635697..636197 (+) 501 WP_341744053.1 CinA family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42231.47 Da        Isoelectric Point: 6.4202

>NTDB_id=976440 AADV58_RS03085 WP_028993620.1 630668..631804(+) (pilU) [Azonexus hydrophilus strain NCP973]
MERDQAMKFMHDLLRLMAQKRGSDLFITSGFPPAIKIDGKITPVSNQVLTSQHTAELARSIMNDRQAAEFESTRECNFAI
SPTGIGRFRVNALVQQGRVAVVCRTINMTIPTLDDLGLPAVVKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENSYG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRDTMDYAIAFAETGHLALATLHANST
NQAIDRIINFFPEERRQQLLMDLSLNVRAMVSQRLLPKKDGKGRVAAVEVMLNSPLISDLIFRGEVQEIKEIMKKSRELG
MQTFDQALFDLYDAGKISYEDALRNADSLNDLRLQIKLHGKESKDRDLTQGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=976440 AADV58_RS03085 WP_028993620.1 630668..631804(+) (pilU) [Azonexus hydrophilus strain NCP973]
ATGGAACGCGATCAGGCAATGAAATTCATGCACGATCTTCTGCGCCTGATGGCGCAGAAGCGTGGTTCGGACTTGTTCAT
CACCTCCGGCTTTCCGCCGGCCATCAAGATCGACGGCAAGATCACGCCGGTCTCCAACCAGGTGCTGACATCGCAGCACA
CGGCCGAACTCGCCCGTTCGATCATGAACGACCGGCAGGCCGCCGAATTCGAGTCGACGCGCGAATGCAACTTCGCCATC
TCGCCGACCGGCATCGGCCGTTTCCGCGTCAATGCGCTGGTTCAGCAGGGGCGCGTCGCGGTTGTCTGCCGGACCATCAA
CATGACCATCCCGACCTTGGACGATCTTGGCCTGCCAGCGGTGGTCAAGGACCTGGCGATGACCAAGCGCGGCCTGATCA
TCTTTGTCGGCGGTACCGGTACCGGCAAGACGACCTCGTTGGCGGCGTTGGTCGATTACCGCAACGAGAACAGCTACGGC
CACATCATCACGATCGAGGACCCGATCGAATACGTGCATGAGCACAAGAACTGCATCGTCACCCAGCGCGAAGTCGGCGT
GGATACCGACGACTGGGGCCCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGACACCATGGACTACGCCATCGCCTTCGCCGAGACCGGCCATCTGGCGCTGGCGACACTGCACGCCAACAGCACC
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGTCAGCAACTGCTGATGGACCTGTCGCTGAATGT
GCGGGCAATGGTTTCGCAGCGCCTGCTGCCGAAGAAGGACGGCAAAGGCCGGGTTGCCGCGGTCGAGGTGATGCTCAACT
CGCCGCTGATTTCCGACCTGATCTTCCGCGGCGAGGTCCAGGAGATCAAGGAAATCATGAAGAAGTCGCGCGAACTCGGC
ATGCAGACCTTCGACCAGGCCTTGTTCGACCTCTACGACGCCGGCAAGATCAGCTACGAGGATGCCTTGCGCAACGCCGA
CTCGCTCAACGACCTGCGCCTCCAGATCAAGTTGCACGGCAAGGAATCCAAGGATCGCGACCTGACCCAGGGCATCGGGC
ATCTCGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.259

96.032

0.598
  pilU Acinetobacter baylyi ADP1

59.889

94.974

0.569
  pilU Vibrio cholerae strain A1552

53.666

90.212

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.624

0.376
  pilT Pseudomonas aeruginosa PAK

42.09

88.624

0.373
  pilT Acinetobacter baylyi ADP1

41.543

89.153

0.37
  pilT Legionella pneumophila strain Lp02

42.296

87.566

0.37
  pilT Legionella pneumophila strain ERS1305867

42.296

87.566

0.37
  pilT Acinetobacter nosocomialis M2

41.692

87.566

0.365
  pilT Acinetobacter baumannii D1279779

41.692

87.566

0.365
  pilT Acinetobacter baumannii strain A118

41.692

87.566

0.365

Multiple sequence alignment