NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	OXA89_RS09540	Genome accession	NZ_CP113434
Coordinates	2118978..2119388 (-)	Length	136 a.a.
NCBI ID	WP_014844212.1	Uniprot ID	A0A378K550
Organism	Legionella pneumophila strain PATHC027
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2113978..2124388

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OXA89_RS09525 (OXA89_09525)	letS	2114296..2117028 (+)	2733	WP_014844211.1	two-component system sensor histidine kinase LetS	Regulator
OXA89_RS09530 (OXA89_09530)	-	2117126..2118370 (+)	1245	WP_011214166.1	6-phosphofructokinase	-
OXA89_RS09535 (OXA89_09535)	pilA2	2118367..2118780 (-)	414	WP_013101573.1	pilin	Machinery gene
OXA89_RS09540 (OXA89_09540)	pilA2	2118978..2119388 (-)	411	WP_014844212.1	pilin	Machinery gene
OXA89_RS09545 (OXA89_09545)	-	2119691..2120008 (+)	318	WP_010947633.1	BolA family protein	-
OXA89_RS09550 (OXA89_09550)	-	2120083..2121504 (+)	1422	WP_014844213.1	APC family permease	-
OXA89_RS09555 (OXA89_09555)	-	2121602..2123032 (-)	1431	WP_027219766.1	cellulase family glycosylhydrolase	-
OXA89_RS09560 (OXA89_09560)	kdsB	2123278..2124030 (-)	753	WP_027219765.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
OXA89_RS09565 (OXA89_09565)	-	2124027..2124206 (-)	180	WP_010947637.1	Trm112 family protein	-

Sequence

Protein

Download Length: 136 a.a. Molecular weight: 13925.15 Da Isoelectric Point: 8.4715

                                >NTDB_id=762037 OXA89_RS09540 WP_014844212.1 2118978..2119388(-) (pilA2) [Legionella pneumophila strain PATHC027]

MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTLADSAKLAVSETAITNNALPATQAATGYVSPAATPNV

QSIAIGANGVITITYTAAAGGGTIIMTPTLQANGDVTWTCTGGTLLAKYRPASCRP

Nucleotide

Download Length: 411 bp

                                >NTDB_id=762037 OXA89_RS09540 WP_014844212.1 2118978..2119388(-) (pilA2) [Legionella pneumophila strain PATHC027]

ATGAGACAAAAGGGTTTTACTTTAATTGAGTTGATGATAGTCGTAGCGATTATCGGTATTTTGGCAGCAATCGCTATACC

AGCTTATCAAGATTACACAATACGGGCTCGTGTTACGGAAGGTCTGACCTTGGCAGATTCTGCAAAACTTGCCGTATCTG

AAACGGCTATTACCAACAACGCGTTACCAGCTACTCAGGCTGCAACGGGCTATGTGTCTCCCGCTGCTACGCCCAATGTA

CAATCAATTGCTATTGGTGCCAACGGTGTGATTACCATAACGTATACCGCTGCAGCAGGTGGTGGTACCATTATTATGAC

CCCAACTTTACAAGCAAACGGTGACGTAACCTGGACTTGTACGGGCGGTACATTATTGGCCAAATACCGACCTGCCAGTT

GCAGACCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A378K550

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila strain ERS1305867	100	100	1
pilA2	Legionella pneumophila str. Paris	98.529	100	0.985
pilA	Ralstonia pseudosolanacearum GMI1000	49.068	100	0.581
comP	Acinetobacter baylyi ADP1	53.103	100	0.566
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.566	100	0.522
pilA/pilA1	Eikenella corrodens VA1	39.873	100	0.463
pilE	Neisseria gonorrhoeae strain FA1090	38.462	100	0.441
pilE	Neisseria gonorrhoeae MS11	38.217	100	0.441
pilA	Haemophilus influenzae 86-028NP	40.816	100	0.441
pilA	Pseudomonas aeruginosa PAK	39.865	100	0.434
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.963	99.265	0.426
pilA	Acinetobacter baumannii strain A118	39.041	100	0.419
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.855	100	0.404
pilA	Haemophilus influenzae Rd KW20	43.307	93.382	0.404
pilA	Vibrio cholerae C6706	37.762	100	0.397
pilA	Vibrio cholerae strain A1552	37.762	100	0.397
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.762	100	0.397
pilA	Glaesserella parasuis strain SC1401	38.235	100	0.382
pilA	Vibrio parahaemolyticus RIMD 2210633	38.462	95.588	0.368

Detailed information NTG0762037