Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TQ33_RS10815 Genome accession   NZ_CP010975
Coordinates   2350815..2351849 (-) Length   344 a.a.
NCBI ID   WP_046562049.1    Uniprot ID   A0A0F6RDL6
Organism   Kangiella geojedonensis strain YCS-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2345815..2356849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQ33_RS10785 (TQ33_2128) pilG 2345999..2346400 (-) 402 WP_046562044.1 twitching motility response regulator PilG Regulator
  TQ33_RS10790 (TQ33_2129) gshB 2346641..2347597 (+) 957 WP_046562045.1 glutathione synthase -
  TQ33_RS10795 (TQ33_2130) - 2347625..2348527 (+) 903 WP_046562046.1 energy transducer TonB -
  TQ33_RS10800 (TQ33_2131) - 2348578..2349141 (+) 564 WP_052735291.1 YqgE/AlgH family protein -
  TQ33_RS10805 (TQ33_2132) ruvX 2349134..2349577 (+) 444 WP_046562047.1 Holliday junction resolvase RuvX -
  TQ33_RS10810 (TQ33_2133) pilU 2349667..2350806 (-) 1140 WP_046562048.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TQ33_RS10815 (TQ33_2134) pilT 2350815..2351849 (-) 1035 WP_046562049.1 type IV pilus twitching motility protein PilT Machinery gene
  TQ33_RS10820 (TQ33_2135) - 2351885..2352571 (+) 687 WP_046562050.1 YggS family pyridoxal phosphate-dependent enzyme -
  TQ33_RS10825 (TQ33_2136) proC 2352702..2353550 (+) 849 WP_046562051.1 pyrroline-5-carboxylate reductase -
  TQ33_RS10830 (TQ33_2137) - 2353591..2354178 (+) 588 WP_046562052.1 YggT family protein -
  TQ33_RS10835 (TQ33_2138) - 2354289..2354750 (+) 462 WP_046562053.1 DUF4426 domain-containing protein -
  TQ33_RS10840 (TQ33_2139) rdgB 2354840..2355439 (+) 600 WP_046562054.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  TQ33_RS10845 (TQ33_2140) hemW 2355439..2356665 (+) 1227 WP_046562055.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38454.13 Da        Isoelectric Point: 6.7300

>NTDB_id=141010 TQ33_RS10815 WP_046562049.1 2350815..2351849(-) (pilT) [Kangiella geojedonensis strain YCS-5]
MDITELLAFSVKNNASDLHISAGMPPMIRVDGDIRRINVPPLEDKEVHSLIYDIMNDKQRKDYEEFLEADFSFELQDLAR
FRVNAFVQNRGRAAVFRTIPSRVLTLDDLKAPKIFQDVSLQPRGLVLVTGPTGSGKSTTLAGMIDYVNDNKHDHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPEGEKMMVRSMLSESLQAVIAQTLLKRNGGGRVAAHEILIGTPAIRNLIREAKVAQMYSAMQTGMSLGMQTLDHSL
KQLVSKGVVSRQDARMKANNKEEF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=141010 TQ33_RS10815 WP_046562049.1 2350815..2351849(-) (pilT) [Kangiella geojedonensis strain YCS-5]
ATGGATATTACAGAGCTTTTAGCGTTCAGTGTAAAGAATAACGCTTCGGATTTACATATCTCTGCTGGTATGCCACCTAT
GATTCGAGTTGATGGCGATATCCGCCGAATTAATGTGCCGCCGCTAGAAGACAAAGAAGTCCACTCGCTGATTTACGACA
TTATGAATGATAAGCAGCGAAAAGACTACGAAGAGTTTCTAGAAGCTGACTTCTCCTTTGAGCTACAGGATTTAGCGCGT
TTCCGTGTTAATGCGTTTGTTCAAAACCGTGGTCGCGCCGCGGTGTTTCGTACCATTCCTTCACGAGTCCTTACGCTAGA
TGACCTGAAGGCACCGAAAATTTTCCAAGATGTTTCGTTACAGCCACGTGGCTTGGTGTTAGTGACAGGGCCAACGGGTT
CCGGTAAGTCAACGACGCTAGCGGGCATGATTGATTATGTGAATGATAATAAACACGACCACATTCTTACTATTGAGGAT
CCGATCGAGTTTGTTCACGAAAGTAAAAAATGTCTGGTTAACCAGCGTGAAGTACACCGTGATACTCATAGCTTCTCAAA
TGCACTACGTTCTGCGTTGCGTGAAGACCCCGATATTATTCTGGTTGGTGAGTTACGTGACTTAGAAACAATCCGCTTAG
CTTTAACGGCAGCAGAAACAGGTCACTTAGTATTCGGTACGCTGCACACCACATCTGCAGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGAAGGCGAAAAGATGATGGTTCGTTCGATGCTATCGGAATCACTACAGGCGGTTATTGCACAGAC
ACTGTTAAAGCGTAATGGTGGTGGTCGTGTGGCGGCGCATGAGATTCTGATTGGTACGCCAGCGATTCGTAACCTGATTC
GTGAAGCCAAGGTTGCGCAAATGTATTCTGCGATGCAAACCGGTATGTCTTTAGGCATGCAGACTCTCGACCACAGTTTG
AAGCAGTTGGTCAGCAAGGGTGTGGTGTCGCGTCAAGATGCGCGAATGAAAGCTAACAATAAAGAAGAATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F6RDL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.942

100

0.799
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Pseudomonas stutzeri DSM 10701

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Legionella pneumophila strain Lp02

73.547

100

0.735
  pilT Legionella pneumophila strain ERS1305867

73.547

100

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.965

0.692
  pilT Vibrio cholerae strain A1552

70.623

97.965

0.692
  pilT Neisseria meningitidis 8013

67.638

99.709

0.674
  pilT Neisseria gonorrhoeae MS11

67.347

99.709

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Vibrio cholerae strain A1552

41.739

100

0.419
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.058

99.419

0.398
  pilB Acinetobacter baylyi ADP1

32.308

100

0.366

Multiple sequence alignment