Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TQ33_RS10810 Genome accession   NZ_CP010975
Coordinates   2349667..2350806 (-) Length   379 a.a.
NCBI ID   WP_046562048.1    Uniprot ID   A0A0F6TSH4
Organism   Kangiella geojedonensis strain YCS-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2344667..2355806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQ33_RS10775 (TQ33_2126) - 2345041..2345589 (-) 549 WP_046562042.1 chemotaxis protein CheW -
  TQ33_RS10780 (TQ33_2127) - 2345596..2345961 (-) 366 WP_046562043.1 response regulator transcription factor -
  TQ33_RS10785 (TQ33_2128) pilG 2345999..2346400 (-) 402 WP_046562044.1 twitching motility response regulator PilG Regulator
  TQ33_RS10790 (TQ33_2129) gshB 2346641..2347597 (+) 957 WP_046562045.1 glutathione synthase -
  TQ33_RS10795 (TQ33_2130) - 2347625..2348527 (+) 903 WP_046562046.1 energy transducer TonB -
  TQ33_RS10800 (TQ33_2131) - 2348578..2349141 (+) 564 WP_052735291.1 YqgE/AlgH family protein -
  TQ33_RS10805 (TQ33_2132) ruvX 2349134..2349577 (+) 444 WP_046562047.1 Holliday junction resolvase RuvX -
  TQ33_RS10810 (TQ33_2133) pilU 2349667..2350806 (-) 1140 WP_046562048.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TQ33_RS10815 (TQ33_2134) pilT 2350815..2351849 (-) 1035 WP_046562049.1 type IV pilus twitching motility protein PilT Machinery gene
  TQ33_RS10820 (TQ33_2135) - 2351885..2352571 (+) 687 WP_046562050.1 YggS family pyridoxal phosphate-dependent enzyme -
  TQ33_RS10825 (TQ33_2136) proC 2352702..2353550 (+) 849 WP_046562051.1 pyrroline-5-carboxylate reductase -
  TQ33_RS10830 (TQ33_2137) - 2353591..2354178 (+) 588 WP_046562052.1 YggT family protein -
  TQ33_RS10835 (TQ33_2138) - 2354289..2354750 (+) 462 WP_046562053.1 DUF4426 domain-containing protein -
  TQ33_RS10840 (TQ33_2139) rdgB 2354840..2355439 (+) 600 WP_046562054.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42819.24 Da        Isoelectric Point: 6.6185

>NTDB_id=141009 TQ33_RS10810 WP_046562048.1 2349667..2350806(-) (pilU) [Kangiella geojedonensis strain YCS-5]
MDITPLLKALMENKGSDLFITVHRPPCIKMHGKIRPVTKSMLTPAQVEELIHSSMDDRQRKEFAENKECQYAISVENVGR
FRASAFYQRNQPGLVVRRIETKIPTTEELKIPSILNDLVMKKRGIIIFVGATGTGKSTSLAAMIGYRNRNSYGHIISIED
PIEYVHRHDKCIITQREVGIDTESFEVALKNTLRQAPDVILIGEVRSQETMSQAITFAETGHLCLCTLHANNANQALDRI
LHFFPKEEHTRVLMDLSMNLRAVIAQQLLPTVDGKGRRAAIEIMINSPLISDHIRKGEVHLIKELMARSTELGMQTFDQA
LFELYVKGEITYDDALVHADSANDLRLRIKLHEKDADSLTDGTEGLQVEMDDDRSSNMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=141009 TQ33_RS10810 WP_046562048.1 2349667..2350806(-) (pilU) [Kangiella geojedonensis strain YCS-5]
ATGGATATTACCCCATTATTAAAAGCGTTGATGGAAAACAAAGGCTCGGACTTGTTCATTACAGTTCATCGTCCGCCTTG
TATCAAAATGCATGGCAAAATTCGCCCTGTTACTAAATCGATGTTAACGCCGGCACAGGTTGAAGAACTGATTCATTCAT
CAATGGATGATCGTCAGAGAAAAGAGTTCGCTGAAAATAAAGAGTGTCAGTACGCAATTAGTGTTGAGAATGTCGGTCGT
TTCCGTGCCAGTGCTTTCTACCAGCGTAACCAACCTGGCCTGGTGGTGCGTCGTATTGAAACCAAGATCCCAACCACTGA
AGAACTTAAAATTCCGAGTATTTTGAATGACTTGGTGATGAAAAAACGCGGAATTATCATTTTCGTTGGTGCGACCGGTA
CTGGTAAGTCAACCTCGCTGGCAGCAATGATAGGTTATCGGAACCGTAATTCTTATGGCCATATCATCAGTATTGAGGAC
CCGATTGAGTATGTACACCGCCATGATAAATGTATTATTACCCAGCGTGAGGTGGGTATCGATACTGAGTCGTTTGAAGT
GGCGCTGAAAAACACTCTACGTCAGGCACCTGATGTTATTCTAATTGGTGAGGTTCGTTCTCAGGAAACCATGTCGCAGG
CGATTACTTTCGCTGAGACTGGTCACTTGTGTTTATGTACTTTGCACGCCAATAACGCCAACCAGGCACTGGATCGTATT
TTGCACTTCTTCCCGAAAGAAGAGCACACTCGAGTGTTAATGGATTTATCGATGAACCTTCGAGCCGTTATTGCACAGCA
GCTACTTCCGACCGTTGATGGTAAAGGGCGTCGAGCAGCGATTGAGATTATGATTAACTCACCATTGATTTCAGATCATA
TCCGCAAAGGTGAAGTGCATCTGATTAAAGAGTTGATGGCACGCTCTACTGAACTCGGTATGCAGACCTTTGACCAAGCC
TTATTTGAGCTTTACGTTAAAGGTGAGATTACGTATGACGATGCGTTAGTCCACGCTGATTCAGCGAACGACTTACGTCT
ACGTATTAAGTTGCATGAGAAAGATGCTGACTCACTTACGGATGGTACTGAAGGCTTGCAGGTTGAGATGGACGACGACC
GCTCAAGCAATATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F6TSH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.789

100

0.66
  pilU Acinetobacter baylyi ADP1

62.817

93.668

0.588
  pilU Vibrio cholerae strain A1552

55.526

97.889

0.544
  pilT Pseudomonas stutzeri DSM 10701

43.582

88.391

0.385
  pilT Acinetobacter baylyi ADP1

42.815

89.974

0.385
  pilT Acinetobacter baumannii D1279779

43.505

87.335

0.38
  pilT Acinetobacter nosocomialis M2

43.505

87.335

0.38
  pilT Acinetobacter baumannii strain A118

43.505

87.335

0.38
  pilT Pseudomonas aeruginosa PAK

42.985

88.391

0.38
  pilT Neisseria meningitidis 8013

41.449

91.029

0.377
  pilT Vibrio cholerae strain A1552

43.333

87.071

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

87.071

0.377
  pilT Neisseria gonorrhoeae MS11

42.388

88.391

0.375
  pilT Legionella pneumophila strain Lp02

42.9

87.335

0.375
  pilT Legionella pneumophila strain ERS1305867

42.9

87.335

0.375
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.003

89.446

0.367

Multiple sequence alignment