Detailed information NTG1147578

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: CKW05_RS10755
• Genome accession: NZ_LT906457
• Coordinates: 2389217..2389624 (-)
• Length: 135 a.a.
• NCBI ID: WP_058482309.1
• Uniprot ID: A0A0W0Z936
• Organism: Legionella spiritensis strain NCTC11990
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2384217..2394624

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKW05_RS10740 (SAMEA4412668_02145)	letS	2384565..2387321 (+)	2757	WP_058482311.1	two-component system sensor histidine kinase LetS	Regulator
CKW05_RS10745 (SAMEA4412668_02146)	-	2387351..2388586 (+)	1236	WP_058482310.1	6-phosphofructokinase	-
CKW05_RS10750 (SAMEA4412668_02147)	pilA2	2388702..2389109 (-)	408	WP_058482456.1	pilin	Machinery gene
CKW05_RS10755 (SAMEA4412668_02148)	pilA2	2389217..2389624 (-)	408	WP_058482309.1	pilin	Machinery gene
CKW05_RS10760 (SAMEA4412668_02149)	-	2389977..2390294 (+)	318	WP_058482308.1	BolA family transcriptional regulator	-
CKW05_RS10765 (SAMEA4412668_02150)	-	2390287..2390730 (+)	444	WP_058482307.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
CKW05_RS10770 (SAMEA4412668_02151)	-	2390804..2392201 (+)	1398	WP_058482455.1	APC family permease	-

Sequence

Protein

• Length: 135 a.a.
• Molecular weight: 13775.87 Da
• Isoelectric Point: 7.7787

>NTDB_id=1147578 CKW05_RS10755 WP_058482309.1 2389217..2389624(-) (pilA2) [Legionella spiritensis strain NCTC11990]
MKQRGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLNLASSAKLAVSETAITNNALPATQAATGYVSPAATANV
TSIAIAANGVITITYTAAAGGGTITMAPTLQANGDVTWDCTGGTLLDKYRPASCR

Nucleotide

• Length: 408 bp

>NTDB_id=1147578 CKW05_RS10755 WP_058482309.1 2389217..2389624(-) (pilA2) [Legionella spiritensis strain NCTC11990]
ATGAAACAGAGAGGTTTTACACTGATTGAGTTGATGATTGTGGTCGCCATTGTAGGTATTCTGGCCGCGATTGCAATACC
GGCCTATCAGGATTATACCATTCGCGCCCGGGTTACCGAGGGATTGAACCTGGCGTCATCAGCCAAACTGGCTGTTTCTG
AAACGGCAATCACTAATAATGCCTTACCGGCTACTCAGGCTGCAACTGGCTATGTTAGTCCTGCCGCAACCGCCAATGTG
ACCTCCATAGCAATCGCCGCGAACGGTGTCATTACTATCACCTATACCGCAGCCGCAGGCGGCGGTACCATCACCATGGC
CCCGACTTTGCAGGCAAACGGTGATGTGACCTGGGATTGCACCGGTGGTACGTTGCTCGATAAATATCGTCCGGCAAGTT
GCAGGTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-25)

• Pilin (bacterial filament) (Pilin) (35-135)

3D structure

• AlphaFold DB: A0A0W0Z936

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila strain ERS1305867	91.111	100	0.911
pilA2	Legionella pneumophila str. Paris	91.111	100	0.911
pilA	Ralstonia pseudosolanacearum GMI1000	49.689	100	0.593
comP	Acinetobacter baylyi ADP1	49.655	100	0.533
pilE	Neisseria gonorrhoeae strain FA1090	38.217	100	0.444
pilA/pilAII	Pseudomonas stutzeri DSM 10701	42.029	100	0.43
pilE	Neisseria gonorrhoeae MS11	37.179	100	0.43
pilA	Pseudomonas aeruginosa PAK	38.514	100	0.422
pilA/pilA1	Eikenella corrodens VA1	38.514	100	0.422
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.741	100	0.407
pilA	Vibrio cholerae C6706	36.986	100	0.4
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.986	100	0.4
pilA	Vibrio cholerae strain A1552	36.986	100	0.4
pilA	Haemophilus influenzae 86-028NP	40.152	97.778	0.393
pilA	Haemophilus influenzae Rd KW20	41.935	91.852	0.385
pilA	Vibrio parahaemolyticus RIMD 2210633	39.231	96.296	0.378
pilA	Acinetobacter baumannii strain A118	44.643	82.963	0.37
comF	Acinetobacter baylyi ADP1	34.965	100	0.37
pilA	Glaesserella parasuis strain SC1401	36.029	100	0.363