Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilT   Type   Machinery gene
Locus tag   DXE37_RS13445 Genome accession   NZ_LT606951
Coordinates   850757..850942 (+) Length   61 a.a.
NCBI ID   WP_269460291.1    Uniprot ID   -
Organism   Polynucleobacter necessarius isolate PPGSP6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 845757..855942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE37_RS04660 - 846274..846690 (+) 417 WP_162786189.1 tetratricopeptide repeat protein -
  DXE37_RS04665 - 846642..847094 (+) 453 WP_162786190.1 hypothetical protein -
  DXE37_RS04670 - 847127..848254 (+) 1128 WP_114636745.1 FecR domain-containing protein -
  DXE37_RS04675 - 848664..848900 (+) 237 WP_231971088.1 hypothetical protein -
  DXE37_RS04680 - 848955..849401 (+) 447 WP_114636747.1 hypothetical protein -
  DXE37_RS04685 - 849422..850303 (+) 882 WP_114636748.1 prepilin peptidase -
  DXE37_RS11260 - 850332..850751 (+) 420 WP_197713079.1 hypothetical protein -
  DXE37_RS13445 pilT 850757..850942 (+) 186 WP_269460291.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DXE37_RS13450 pilT 850911..851123 (+) 213 WP_269460292.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DXE37_RS12455 pilT 851123..851335 (+) 213 WP_231971091.1 hypothetical protein Machinery gene
  DXE37_RS04695 - 851332..851892 (+) 561 WP_114636749.1 Tfp pilus assembly protein FimT/FimU -
  DXE37_RS04700 - 851892..852278 (+) 387 WP_114636750.1 hypothetical protein -
  DXE37_RS04705 - 852349..852792 (+) 444 WP_114636751.1 PilW family protein -
  DXE37_RS04710 - 853030..853638 (+) 609 WP_114636752.1 PilX N-terminal domain-containing pilus assembly protein -
  DXE37_RS04715 - 853642..855030 (+) 1389 WP_114636753.1 hypothetical protein -
  DXE37_RS13455 - 855055..855489 (+) 435 WP_114636754.1 PilC/PilY family type IV pilus protein -
  DXE37_RS13460 - 855495..855842 (+) 348 WP_162786191.1 PilC/PilY family type IV pilus protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 7131.14 Da        Isoelectric Point: 6.7066

>NTDB_id=1145230 DXE37_RS13445 WP_269460291.1 850757..850942(+) (pilT) [Polynucleobacter necessarius isolate PPGSP6]
MIDRINRTRHEHIITIEDPIEFIHQNQKCVVSQCEVKRDTLSFSSALRASLREDPDVILGR

Nucleotide


Download         Length: 186 bp        

>NTDB_id=1145230 DXE37_RS13445 WP_269460291.1 850757..850942(+) (pilT) [Polynucleobacter necessarius isolate PPGSP6]
ATGATTGATCGAATTAACCGTACTCGTCATGAACATATCATCACGATTGAAGATCCGATTGAGTTTATTCATCAAAACCA
AAAATGCGTTGTATCCCAATGTGAGGTGAAAAGAGATACCTTATCTTTTTCTTCCGCATTACGCGCCTCTTTACGTGAAG
ATCCAGACGTTATCTTGGGTAGGTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

66.102

96.721

0.639

  pilT Legionella pneumophila strain Lp02

66.102

96.721

0.639

  pilT Pseudomonas stutzeri DSM 10701

64.407

96.721

0.623

  pilT Pseudomonas aeruginosa PAK

64.407

96.721

0.623

  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.712

96.721

0.607

  pilT Vibrio cholerae O1 biovar El Tor strain E7946

61.017

96.721

0.59

  pilT Neisseria meningitidis 8013

61.017

96.721

0.59

  pilT Vibrio cholerae strain A1552

61.017

96.721

0.59

  pilT Acinetobacter baumannii D1279779

61.017

96.721

0.59

  pilT Acinetobacter baumannii strain A118

61.017

96.721

0.59

  pilT Acinetobacter nosocomialis M2

61.017

96.721

0.59

  pilT Acinetobacter baylyi ADP1

61.017

96.721

0.59

  pilT Neisseria gonorrhoeae MS11

59.322

96.721

0.574

  pilU Pseudomonas stutzeri DSM 10701

59.322

96.721

0.574

  pilU Acinetobacter baylyi ADP1

55.932

96.721

0.541

  pilU Vibrio cholerae strain A1552

53.704

88.525

0.475

  pilB Haemophilus influenzae 86-028NP

44.231

85.246

0.377

  pilB Acinetobacter baylyi ADP1

41.071

91.803

0.377

  pilB/pilB1 Synechocystis sp. PCC 6803

44.898

80.328

0.361


Multiple sequence alignment