Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACLB90_RS05225 Genome accession   NZ_CP177163
Coordinates   1243701..1244138 (-) Length   145 a.a.
NCBI ID   WP_411851930.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LGBM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1238701..1249138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB90_RS05210 (ACLB90_05210) pilB 1239711..1241441 (-) 1731 WP_411851927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLB90_RS05215 (ACLB90_05215) - 1241526..1242398 (-) 873 WP_411851928.1 serine hydrolase domain-containing protein -
  ACLB90_RS05220 (ACLB90_05220) - 1242425..1243414 (-) 990 WP_411851929.1 methyltransferase family protein -
  ACLB90_RS05225 (ACLB90_05225) pilA2 1243701..1244138 (-) 438 WP_411851930.1 pilin Machinery gene
  ACLB90_RS05230 (ACLB90_05230) pilC 1244500..1245759 (+) 1260 WP_411851931.1 type II secretion system F family protein Machinery gene
  ACLB90_RS05235 (ACLB90_05235) pilD 1245767..1246630 (+) 864 WP_017356176.1 prepilin peptidase Machinery gene
  ACLB90_RS05240 (ACLB90_05240) coaE 1246641..1247258 (+) 618 WP_411851932.1 dephospho-CoA kinase -
  ACLB90_RS05245 (ACLB90_05245) - 1247278..1248609 (-) 1332 WP_411851933.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14755.93 Da        Isoelectric Point: 9.0250

>NTDB_id=1085704 ACLB90_RS05225 WP_411851930.1 1243701..1244138(-) (pilA2) [Stenotrophomonas sp. LGBM10]
MKNQKGFTLIELMIVVAIIAILAAIALPAYQDYLVKSRVSEVMLAASSARTTVSEAAAADTANATAMPGTVDIESQSSKY
VSGVAYANGRITATGRALGGTTPTGTIILVGNKSANGQVEWTCTGTIAAKYRPATCQGALPTTGG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1085704 ACLB90_RS05225 WP_411851930.1 1243701..1244138(-) (pilA2) [Stenotrophomonas sp. LGBM10]
ATGAAGAATCAGAAGGGCTTCACCCTGATCGAACTGATGATCGTCGTCGCGATCATTGCCATCCTGGCTGCCATCGCGCT
GCCGGCCTACCAGGATTACCTGGTCAAGTCGCGCGTGTCCGAAGTGATGCTGGCCGCTTCTTCGGCCCGCACCACCGTGT
CGGAAGCCGCTGCTGCCGATACGGCGAATGCCACCGCGATGCCGGGCACTGTCGACATCGAATCCCAGTCCTCCAAGTAC
GTCTCCGGCGTTGCTTACGCCAACGGCCGTATCACTGCCACGGGCCGGGCTCTGGGCGGCACCACTCCGACCGGCACCAT
CATCCTGGTCGGCAACAAGAGTGCCAACGGTCAGGTTGAATGGACCTGCACCGGCACCATCGCTGCCAAGTACCGCCCGG
CCACCTGCCAGGGCGCGCTGCCGACCACCGGCGGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.206

93.793

0.49
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.587

100

0.49
  pilA2 Legionella pneumophila str. Paris

51.471

93.793

0.483
  comP Acinetobacter baylyi ADP1

45.395

100

0.476
  pilA Ralstonia pseudosolanacearum GMI1000

39.759

100

0.455
  pilA/pilA1 Eikenella corrodens VA1

38.854

100

0.421
  pilE Neisseria gonorrhoeae MS11

37.736

100

0.414
  pilA Acinetobacter baumannii strain A118

37.419

100

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.912

93.793

0.393
  pilE Neisseria gonorrhoeae strain FA1090

34.969

100

0.393
  pilA Vibrio cholerae strain A1552

35.669

100

0.386
  pilA Vibrio cholerae C6706

35.669

100

0.386
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.669

100

0.386
  pilA Haemophilus influenzae Rd KW20

40

96.552

0.386
  pilA Pseudomonas aeruginosa PAK

36.424

100

0.379
  pilA Haemophilus influenzae 86-028NP

39.855

95.172

0.379

Multiple sequence alignment