Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLB90_RS05210 Genome accession   NZ_CP177163
Coordinates   1239711..1241441 (-) Length   576 a.a.
NCBI ID   WP_411851927.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LGBM10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1234711..1246441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB90_RS05195 (ACLB90_05195) sucC 1235117..1236286 (-) 1170 WP_046273840.1 ADP-forming succinate--CoA ligase subunit beta -
  ACLB90_RS05200 (ACLB90_05200) - 1236606..1238219 (+) 1614 WP_411851925.1 sensor histidine kinase -
  ACLB90_RS05205 (ACLB90_05205) pilR 1238298..1239686 (+) 1389 WP_411851926.1 sigma-54-dependent transcriptional regulator Regulator
  ACLB90_RS05210 (ACLB90_05210) pilB 1239711..1241441 (-) 1731 WP_411851927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLB90_RS05215 (ACLB90_05215) - 1241526..1242398 (-) 873 WP_411851928.1 serine hydrolase domain-containing protein -
  ACLB90_RS05220 (ACLB90_05220) - 1242425..1243414 (-) 990 WP_411851929.1 methyltransferase family protein -
  ACLB90_RS05225 (ACLB90_05225) pilA2 1243701..1244138 (-) 438 WP_411851930.1 pilin Machinery gene
  ACLB90_RS05230 (ACLB90_05230) pilC 1244500..1245759 (+) 1260 WP_411851931.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62301.35 Da        Isoelectric Point: 6.0484

>NTDB_id=1085703 ACLB90_RS05210 WP_411851927.1 1239711..1241441(-) (pilB) [Stenotrophomonas sp. LGBM10]
MSVVSTANLVGITGIARRLVQDGALDEAAARDAMAKAAAAKQPLPQWFAEHKLVTAGQLAAANALEFGMPLLDVSVFDGN
QNAISLVSEELLRKHRVLPLFKRGGKLFVGTSDPTHSLDEIKFHTNLVVEPILVDEEQIKRTLEQWQSSHDTLGSALGGG
DDEGMGDLDVSSGDEEGGSPDAGVDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRFRIDGLLKNVAKAPVK
LNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPNQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPFNITSSVTLVIAQRLARRLCGNCKRPIELPAH
ALLAEGFTQDQIDAGIRLYEPVGCEECTEGYKGRTGIYQVMPMSEEISVIVLAGGNAIQIAEAAQRAGVNDLRQSALVKA
AAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1085703 ACLB90_RS05210 WP_411851927.1 1239711..1241441(-) (pilB) [Stenotrophomonas sp. LGBM10]
ATGTCCGTAGTCAGCACCGCCAACCTCGTCGGCATCACCGGCATCGCACGTCGCCTGGTCCAGGACGGCGCGCTCGACGA
GGCGGCCGCGCGCGACGCGATGGCCAAGGCCGCGGCCGCCAAGCAGCCGCTGCCGCAGTGGTTTGCCGAGCACAAGCTGG
TCACTGCCGGGCAGCTGGCGGCGGCCAACGCCCTGGAGTTCGGCATGCCGCTGCTGGACGTGTCGGTGTTCGATGGCAAC
CAGAACGCGATCAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACCGCGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGCACCAGCGACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TCGATGAGGAGCAGATCAAGCGCACCCTGGAGCAGTGGCAGTCCAGTCATGACACCCTCGGCTCGGCGCTCGGTGGCGGC
GACGACGAGGGCATGGGCGATCTGGATGTCTCCAGCGGCGACGAGGAGGGCGGCAGCCCGGATGCCGGCGTGGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGTGCCTCGGACATCCATT
TCGAGCCCTACGAAGACGATTACCGGGTGCGCTTCCGCATCGACGGCCTGCTCAAGAACGTGGCCAAGGCGCCGGTCAAG
CTCAACCAGCGCATCGCTGCACGTCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGGCG
CATCAAGCTCAACCTGTCCAAGACCCGGCAGATCGACTTCCGCGTCAGCACGCTGCCAACGCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTGGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGAACCAGCAGAAGCTGTTC
CTGGAAGCCATCCACAAGCCCTACGGGATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCATACCAACGACGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCTCCGTTCAACATCACCAGTT
CGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCGGCAACTGCAAGCGGCCGATCGAGCTGCCCGCGCAT
GCGCTGCTGGCCGAGGGCTTCACCCAGGACCAGATCGATGCCGGGATCCGGCTGTACGAGCCGGTGGGCTGCGAGGAATG
CACCGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGAGCGAAGAGATTTCGGTGATCGTGCTGGCCG
GCGGCAACGCCATCCAGATCGCCGAAGCGGCGCAGCGCGCCGGGGTCAATGACCTGCGCCAGTCGGCGCTGGTGAAGGCC
GCGGCTGGCGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-151)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.316

98.958

0.557
  pilB Acinetobacter baumannii D1279779

56.514

98.611

0.557
  pilB Legionella pneumophila strain ERS1305867

51.761

98.611

0.51
  pilF Neisseria gonorrhoeae MS11

49.296

98.611

0.486
  pilB Vibrio cholerae strain A1552

49.548

96.007

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

47.857

97.222

0.465
  pilB Vibrio campbellii strain DS40M4

45.709

99.132

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

36.125

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.122

94.965

0.372
  pilF Thermus thermophilus HB27

39.888

92.708

0.37

Multiple sequence alignment