Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB1S55_RS05200 Genome accession   NZ_CP166840
Coordinates   1158792..1159826 (+) Length   344 a.a.
NCBI ID   WP_370980730.1    Uniprot ID   -
Organism   Agaribacterium sp. ZY112     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1153792..1164826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1S55_RS05170 (AB1S55_05160) - 1154291..1154731 (-) 441 WP_370980724.1 DUF4426 domain-containing protein -
  AB1S55_RS05175 (AB1S55_05165) metW 1154733..1155326 (-) 594 WP_370980725.1 methionine biosynthesis protein MetW -
  AB1S55_RS05180 (AB1S55_05170) - 1155326..1156468 (-) 1143 WP_370980726.1 homoserine O-acetyltransferase -
  AB1S55_RS05185 (AB1S55_05175) - 1156472..1157059 (-) 588 WP_370980727.1 YggT family protein -
  AB1S55_RS05190 (AB1S55_05180) proC 1157056..1157889 (-) 834 WP_370980728.1 pyrroline-5-carboxylate reductase -
  AB1S55_RS05195 (AB1S55_05185) - 1157926..1158624 (-) 699 WP_370980729.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1S55_RS05200 (AB1S55_05190) pilT 1158792..1159826 (+) 1035 WP_370980730.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1S55_RS05205 (AB1S55_05195) pilU 1159856..1161004 (+) 1149 WP_370980732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1S55_RS05210 (AB1S55_05200) ruvX 1161013..1161456 (-) 444 WP_370980733.1 Holliday junction resolvase RuvX -
  AB1S55_RS05215 (AB1S55_05205) - 1161461..1162045 (-) 585 WP_370980734.1 YqgE/AlgH family protein -
  AB1S55_RS05220 (AB1S55_05210) - 1162126..1162995 (-) 870 WP_370980735.1 energy transducer TonB -
  AB1S55_RS05225 (AB1S55_05215) gshB 1163000..1163920 (-) 921 WP_370981573.1 glutathione synthase -
  AB1S55_RS05230 (AB1S55_05220) pilG 1164235..1164615 (+) 381 WP_370980736.1 twitching motility response regulator PilG Regulator

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38210.99 Da        Isoelectric Point: 6.8365

>NTDB_id=1035609 AB1S55_RS05200 WP_370980730.1 1158792..1159826(+) (pilT) [Agaribacterium sp. ZY112]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINVPAMKHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGQVFRDISAVPRGLVLVTGPTGSGKSTTLAAMMDYINESKYEHILTIED
PIEFVHESKKCLVNQREVHKDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAAEKAMVRSMLSESLQAVISQTLMKRQGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTMDQCL
SDLVERRIITRDVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1035609 AB1S55_RS05200 WP_370980730.1 1158792..1159826(+) (pilT) [Agaribacterium sp. ZY112]
ATGGATATTACGGAGTTGCTGGCTTTTTCCGCCAAGCAGGGGGCATCAGATTTACACCTAAGTGCGGGTTTGCCGCCGAT
GATCCGTGTGGATGGCGACGTGCGCCGTATCAATGTTCCCGCTATGAAACACAAGCAGGTACACGGCCTAATTTATGAAA
TCATGAACGATAAGCAGCGCAAAGATTATGAAGAGTTTCTTGAAACGGACTTTTCATTTGAAGTGCCTGGAGTGGCTCGT
TTCCGAGTCAATGCATTTAACCAAAACCGCGGTGCAGGGGCGGTTTTTCGTACTATTCCCTCCAAGGTTTTAACTATGGA
AGAGCTTGGTATGGGTCAAGTTTTTCGCGATATTTCGGCGGTTCCACGGGGGTTGGTCTTAGTAACAGGGCCGACGGGGT
CGGGTAAATCGACCACGCTTGCGGCAATGATGGATTACATCAATGAAAGTAAGTATGAGCACATACTAACCATTGAGGAT
CCAATTGAATTTGTGCACGAGAGTAAGAAGTGCTTGGTCAACCAGCGTGAGGTTCACAAAGATACCCACGGCTTCGCAGA
GGCTTTACGTTCTGCCTTACGTGAAGATCCCGACATTATTCTTGTAGGTGAGATGCGAGACCTTGAAACGATTCGTTTGG
CCTTAACCGCTGCAGAAACAGGACACTTAGTATTTGGCACCTTGCATACTACGTCTGCAGCTAAAACCATCGACCGTGTT
GTTGATGTATTTCCTGCTGCAGAAAAAGCAATGGTGCGTTCTATGCTGTCTGAGTCATTACAGGCTGTGATCTCGCAGAC
ATTAATGAAGCGCCAAGGTGGCGGCCGTGTAGCTGCGCATGAGATTATGCGTGGTACACCAGCAATTCGTAATTTGATAC
GTGAAGATAAGGTCGCGCAGATGTATTCTGCGATCCAAACTGGCGGCTCACTTGGCATGCAAACCATGGATCAATGTTTG
TCCGATCTTGTCGAGCGCCGCATCATTACCCGTGATGTGGCACGAGAAAAAGCGAAAATGCCAGAGAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.663

100

0.887
  pilT Acinetobacter nosocomialis M2

82.558

100

0.826
  pilT Acinetobacter baumannii D1279779

82.558

100

0.826
  pilT Acinetobacter baumannii strain A118

82.558

100

0.826
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.64

0.674
  pilT Vibrio cholerae strain A1552

70.517

95.64

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.547

0.404
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Vibrio cholerae strain A1552

40.615

94.477

0.384

Multiple sequence alignment