Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB1S55_RS05205 Genome accession   NZ_CP166840
Coordinates   1159856..1161004 (+) Length   382 a.a.
NCBI ID   WP_370980732.1    Uniprot ID   -
Organism   Agaribacterium sp. ZY112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1154856..1166004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1S55_RS05180 (AB1S55_05170) - 1155326..1156468 (-) 1143 WP_370980726.1 homoserine O-acetyltransferase -
  AB1S55_RS05185 (AB1S55_05175) - 1156472..1157059 (-) 588 WP_370980727.1 YggT family protein -
  AB1S55_RS05190 (AB1S55_05180) proC 1157056..1157889 (-) 834 WP_370980728.1 pyrroline-5-carboxylate reductase -
  AB1S55_RS05195 (AB1S55_05185) - 1157926..1158624 (-) 699 WP_370980729.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1S55_RS05200 (AB1S55_05190) pilT 1158792..1159826 (+) 1035 WP_370980730.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1S55_RS05205 (AB1S55_05195) pilU 1159856..1161004 (+) 1149 WP_370980732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1S55_RS05210 (AB1S55_05200) ruvX 1161013..1161456 (-) 444 WP_370980733.1 Holliday junction resolvase RuvX -
  AB1S55_RS05215 (AB1S55_05205) - 1161461..1162045 (-) 585 WP_370980734.1 YqgE/AlgH family protein -
  AB1S55_RS05220 (AB1S55_05210) - 1162126..1162995 (-) 870 WP_370980735.1 energy transducer TonB -
  AB1S55_RS05225 (AB1S55_05215) gshB 1163000..1163920 (-) 921 WP_370981573.1 glutathione synthase -
  AB1S55_RS05230 (AB1S55_05220) pilG 1164235..1164615 (+) 381 WP_370980736.1 twitching motility response regulator PilG Regulator
  AB1S55_RS05235 (AB1S55_05225) pilH 1164664..1165026 (+) 363 WP_370980737.1 twitching motility response regulator PilH -
  AB1S55_RS05240 (AB1S55_05230) - 1165032..1165574 (+) 543 WP_370980738.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42546.61 Da        Isoelectric Point: 5.9827

>NTDB_id=1035610 AB1S55_RS05205 WP_370980732.1 1159856..1161004(+) (pilU) [Agaribacterium sp. ZY112]
MELDKLLSLMVEKKASDLFITAGVPPSIKVHGRIVPVSSTPVSPEKARETVLSVMNERQRKEFVEKKELNFAVSARGIGR
FRTSAFFQRNLAGMVLRRIETEIPTVDGLGLPEVIKELAMTKRGLVMFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFMHQHNGCVITQREVGIDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHDQLWMDLSLNLKGIVAQQLVPTPDGSGRRACLEVFLNTPLAADLIRKGAVHELKDLMTRSTDLGMQTFDQA
LYELYDSGEITYEDALSYADSPNDLRLRIKLGSETDSEYLSGAADEFEVEEDERNAGIRRRF

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=1035610 AB1S55_RS05205 WP_370980732.1 1159856..1161004(+) (pilU) [Agaribacterium sp. ZY112]
ATGGAACTCGACAAGCTCTTGTCGCTCATGGTTGAAAAGAAGGCCTCGGACTTATTTATTACCGCAGGCGTACCGCCTTC
AATTAAGGTTCACGGCCGGATTGTTCCTGTGAGTTCGACTCCGGTGTCGCCAGAAAAGGCGCGTGAAACCGTCTTGAGCG
TTATGAACGAGCGTCAGCGCAAAGAGTTTGTTGAGAAAAAAGAGCTCAACTTTGCAGTGTCTGCACGTGGTATTGGTCGT
TTTCGAACCAGTGCGTTTTTCCAGCGTAATTTGGCTGGCATGGTCCTACGTCGTATTGAAACTGAAATTCCTACTGTTGA
TGGCTTGGGTTTACCTGAGGTCATTAAAGAGTTGGCGATGACTAAGCGTGGCTTGGTTATGTTTGTTGGAGCAACAGGCA
CTGGTAAGTCGACTTCTTTGGCGGCGATGATTGGCCATCGCAACCGTAATTCCAAGGGGCATATTATCTCTATTGAGGAC
CCGATCGAGTTTATGCATCAGCACAATGGCTGTGTTATTACTCAGCGAGAGGTCGGTATTGATACGGAGAGCTTTGAAGT
TGCACTTAAAAACACCTTGCGCCAAGCTCCCGATGTGATTTTGATCGGTGAGGTTCGCTCACGTGAAACGATGGATCATG
CCATTGCCTTTGCTGAGACGGGGCACTTATGTCTTTGTACTTTGCATGCCAATAATGCAAACCAAGCTTTGGATAGAATT
ATTCATTTTTTCCCTGCTGATAGGCACGATCAATTATGGATGGATTTAAGCTTGAATCTTAAAGGCATTGTGGCCCAGCA
ATTGGTTCCAACACCCGATGGTAGTGGCCGTCGTGCGTGTTTGGAGGTGTTTTTGAATACGCCTTTAGCAGCTGACCTTA
TTCGCAAGGGTGCCGTGCACGAGCTAAAAGACTTAATGACGCGCTCAACCGATTTGGGTATGCAAACCTTTGATCAGGCG
CTTTACGAGCTTTATGACTCTGGTGAAATTACCTATGAAGACGCATTAAGCTACGCCGACTCTCCTAATGACCTTCGCTT
GCGTATTAAGCTGGGTTCAGAAACCGATTCTGAGTATTTGTCTGGTGCTGCGGATGAGTTTGAGGTCGAAGAGGATGAGC
GTAATGCCGGAATACGCCGCCGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.719

98.691

0.767
  pilU Acinetobacter baylyi ADP1

64.426

93.455

0.602
  pilU Vibrio cholerae strain A1552

55.135

96.859

0.534
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

90.314

0.387
  pilT Legionella pneumophila strain Lp02

41.791

87.696

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.696

0.366

Multiple sequence alignment